FACSAria Fusion Cell Sorter

FACSAria 融合细胞分选仪

基本信息

批准号：
9075287
负责人：
XUEDONG LIU
金额：
$ 59.95万
依托单位：
UNIVERSITY OF COLORADO
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-04-01 至 2017-03-31
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): We request funds to purchase a high-performance cell sorter coupled with an integrated biological safety cabinet (BSC) to support innovative research by investigators at the University of Colorado-Boulder (UCB). The Becton, Dickinson and Company (BD) FACSAria Fusion is a state-of-the-art high-performance cell sorter capable of multicolor, analyzer-like sensitivity in a custom-fitted biological safety cabinet, designed by The Baker Company, for optimized performance and safety. The proposed instrument will replace a MoFlo Legacy sorter that has been in continuous operation for 16 years in the core facility. Due to the unavailability of parts, and termination of service, the unit can no longer be reliably operated and will soon need to be retired. The past several years CU-Boulder has seen significant growth in the field of biomedical research. Innovations in fluorescence technologies and cell biology during the past decade have dramatically increased the demand of cell sorting capabilities. Our core facility has fallen far behind in meeting the needs of our users due to the age and severe hardware limitations of the MoFlo Legacy sorter. These deficiencies hamper the growth of the core facility to serve the growing requirements of the numerous NIH funded researchers located within JSCBB and throughout the Boulder scientific community. The proposed equipment will allow us to: 1) continue to provide quality services to our existing core user base; 2) address unmet research need to sort and analyze pathogenic and infectious specimens. A number of laboratories in our building and on campus conduct research in this area and there is no cell sorter in the greater Boulder area that has the ability or proper enclosure to sort at biosafety level (BSL) 2+ or 3. This provision is critical for the safe operatin of research goals contained in this proposal; 3) expand our capabilities to serve investigators working on bacteria and yeast. Currently we are the only core facility on campus that serves this group of users; 4) increase our capabilities for multicolor cell sorting and fluorescence modalities such as FRET. Our Legacy sorter can only sort in two wavelengths and there is no cell sorter on campus or nearby with 445 nm and 561 nm laser excitation capabilities, which are ideal for constructs that utilize Cyan and/or Red fluorescent proteins. The replacement of our existing technology with the BD FACSAria Fusion is sorely needed to continue supporting the broad range of NIH funded cutting-edge biomedical research at CU- Boulder. With the new instrument and capabilities, it will empower the users to push new boundaries in their research and spur innovation.

描述（由申请人提供）：我们请求资金购买配备集成生物安全柜 (BSC) 的高性能细胞分选机，以支持科罗拉多大学博尔德分校 (UCB)、The Becton 和 Dickinson 的研究人员进行创新研究。公司 (BD) FACSAria Fusion 是一款最先进的高性能细胞分选仪，由 The Baker Company 设计，在定制的生物安全柜中具有多色、分析仪般的灵敏度，可优化性能拟议的仪器将取代在核心设施中连续运行 16 年的 MoFlo Legacy 分拣机，由于零件不可用且服务终止，该装置无法再运行。在过去的几年里，科罗拉多大学博尔德分校的生物医学研究领域取得了显着的发展，这极大地增加了我们对细胞分选能力的需求。由于 MoFlo Legacy 分类器的老化和严格的硬件限制，核心设施远远落后于满足用户的需求。这些缺陷阻碍了核心设施的发展，无法满足 JSCBB 资助的众多 NIH 研究人员不断增长的需求。以及自始至终拟议的设备将使我们能够：1) 继续为我们现有的核心用户群提供优质服务；2) 解决我们大楼和实验室中未满足的研究需求，以分类和分析病原性和传染性标本。在校园内进行该领域的研究，并且大博尔德地区没有细胞分选机有能力或适当的外壳在生物安全级别 (BSL) 2+ 或 3 下进行分选。这项规定对于安全运行所包含的研究目标至关重要在该提案中；3）扩大我们为从事细菌和酵母研究的研究人员提供服务的能力；4）我们增强了多色细胞分选和荧光模式（例如 FRET）的能力。我们的传统分选机只能以两种波长进行分选，并且校园内或附近没有具有 445 nm 和 561 nm 激光激发功能的细胞分选机，这是利用青色和/或红色荧光蛋白的构建体的理想替代品。迫切需要 BD FACSAria Fusion 的现有技术来继续支持 NIH 资助的科罗拉多大学博尔德分校的广泛的尖端生物医学研究，借助新的仪器和功能，它将帮助用户在研究中突破新的界限并刺激。创新。