A FACILITY FOR S. POMBE MICROARRAYS

粟酒裂殖酵母微阵列设备

• 批准号: 6540707
• 负责人: JANET K LEATHERWOOD
• 金额: $ 19.65万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-16 至 2005-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6540707
• 关键词: Schizosaccharomyces pombe; biomedical facility; complementary DNA; microarray technology

DESCRIPTION (provided by applicant): The genomic sequence of the fission yeast S. pombe will soon be published. This yeast provides a powerful genetic and molecular system, which is currently the major focus of study for about 40 laboratories in the U.S., and an even larger number in Europe and Japan. Many of the laboratories in the U.S., including those of Drs. Janet Leatherwood and A. Bruce Futcher, work on the cell cycle, for which S. pombe is an excellent model organism. Spotted cDNA microarrays are a cheap, rapid, efficient method for collecting data and investigating many subjects from cell physiology and transcription circuits to chromosome structure, mRNA splicing, translational regulation, DNA replication control, and meiotic recombination. Here, it is proposed to create a facility for the production and use of S. pombe cDNA microarrays. These microarrays would be available to the community of S. pombe workers in the U.S., and, if resources permit, to workers in other countries. Microarrays would be made at a facility at the SUNY, Stony Brook. Outside users of the facility would do experiments designed for microarray analysis, isolate RNA (or DNA), validate the samples, and convert the RNA to labeled cDNA or label the isolated DNA. This labeled DNA would be sent to the facility, where it would be hybridized to the microarrays, scanned, and subjected to standard forms of analysis. Results would be posted to a Web site for further analysis by the user. Resources for more extensive data analysis would be available on the facility?s Web site.

描述（由申请人提供）：裂变的基因组序列 粟酒裂殖酵母酵母即将出版。这种酵母提供了强大的遗传 和分子系统，这是目前约 40 个研究的主要焦点 美国的实验室，欧洲和日本的实验室数量甚至更多。许多 美国的实验室，包括博士的实验室。珍妮特·莱瑟伍德 和 A. Bruce Futcher，研究细胞周期，粟酒裂殖酵母是其中的一个 优秀的模式生物。斑点 cDNA 微阵列是一种廉价、快速、 从细胞中收集数据和研究许多受试者的有效方法 染色体结构的生理学和转录回路、mRNA 剪接、 翻译调控、DNA 复制控制和减数分裂重组。 在这里，建议建立一个生产和使用 S 的设施。 粟酒 cDNA 微阵列。这些微阵列将提供给社区 美国的粟酒裂殖酵母工人，如果资源允许，其他国家的工人 国家。微阵列将在纽约州立大学石溪分校的一个设施中制造。 该设施的外部用户将进行为微阵列设计的实验 分析、分离 RNA（或 DNA）、验证样品并将 RNA 转化为 标记 cDNA 或标记分离的 DNA。该标记的 DNA 将被发送至 设施，在那里它将与微阵列杂交、扫描和 接受标准形式的分析。结果将发布到网络上 供用户进一步分析的网站。更广泛数据的资源 分析结果可在该机构的网站上获取。