Novel Mechanisms of Gene Regulation in Meiosis: Antisense RNAs and RNA Processing

减数分裂中基因调控的新机制：反义RNA和RNA加工

基本信息

批准号：
7917081
负责人：
JANET K LEATHERWOOD
金额：
$ 34.35万
依托单位：
STATE UNIVERSITY NEW YORK STONY BROOK
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-04-01 至 2015-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7917081
关键词：
Accounting Affect Antisense RNA Binding Biological Models Cell Cycle Cells Chromatin Collaborations Complement Congenital Abnormality Coupled Event Fission Yeast Functional RNA Gene Expression Gene Expression Profile Gene Expression Regulation Gene Targeting Genes Genetic Screening Genetic Transcription Growth Human Infertility Investigation Mediating Meiosis Messenger RNA Mutation Open Reading Frames Pathway interactions Pattern Phase Polyadenylation Polyadenylation Pathway Process Proteins RNA RNA 3&apos End Processing RNA Interference RNA Polymerase II RNA Processing RNA Splicing RNA-Binding Proteins Regulation Reporter Genes Repression Role Saccharomyces cerevisiae Saccharomycetales Screening procedure Testing Transcript Variant base carcinogenesis density forkhead protein human disease mutant novel programs research study transcription factor

项目摘要

The fission yeast S. pombe, like S. cerevisiae, has a highly regulated gene expression program for meiosis. However, it appears that a significant portion of the meiotic regulation is not at the transcriptional level, but rather post-transcriptional, at the level of RNA processing. S. pombe has a sophisticated apparatus for RNA processing, including regulation of splicing, and including RNAi. Here, we will focus on these RNA-mediated mechanisms of meiotic regulation, to complement investigations of transcription-factor based regulation in S. cerevisiae. We will provide a detailed characterization of the meiotic transcriptome. We will investigate the inter-related effects of 3' end processing (e.g., cleavage and polyadenylation), RNA turnover, and RNA splicing, and how these processes and the Mmil protein combine to express the early meiotic genes. We will investigate the roles of the anti-sense transcripts found over many of the middle meiotic genes. Finally, in collaboration with Drs. Futcher, Neiman, and Sternglanz we will identify the RNA targets of a number of important meiotic RNA binding proteins in both S. pombe and S. cerevisiae.

裂殖酵母粟酒裂殖酵母与酿酒酵母一样，具有高度调控的减数分裂基因表达程序。然而，减数分裂调节的很大一部分似乎不是在转录水平，而是在转录后的RNA加工水平。粟酒裂殖酵母具有复杂的 RNA 加工装置，包括剪接调节和 RNAi。在这里，我们将重点关注这些 RNA 介导的减数分裂调节机制，以补充酿酒酵母中基于转录因子的调节的研究。我们将提供减数分裂转录组的详细表征。我们将研究 3' 末端加工（例如切割和聚腺苷酸化）、RNA 周转和 RNA 剪接的相互关联的影响，以及这些过程和 Mmil 蛋白如何结合来表达早期减数分裂基因。我们将研究在许多减数分裂中期基因上发现的反义转录本的作用。最后，与博士合作。 Futcher、Neiman 和 Sternglanz 我们将鉴定许多 RNA 靶点粟酒裂殖酵母和酿酒酵母中重要的减数分裂 RNA 结合蛋白。