Measurement of biomolecular interactions and association via light scattering

通过光散射测量生物分子相互作用和缔合

• 批准号: 9148733
• 负责人: Allen P Minton
• 金额: $ 18万
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9148733
• 关键词: Charge; Dependence; Individual; Laboratories; Measurement; Measures; Modeling; Ovalbumin; Ovomucin; Pancreatic ribonuclease; Proteins; Ribonucleases; Thermodynamics; Ursidae Family; light scattering; particle

The concentration dependence of light scattering of three proteins, ovalbumin, ribonuclease A, and ovomucoid, and binary mixtures of the three proteins were measured at various compositions and pH values at total protein concentrations of up to 35 g/l, under conditions such that ribonuclease bears either a net positive or negative charge and the other proteins bear a net negative charge. The concentration dependence was interpreted in the context of three models: a statistical thermodynamic model, an extension of the effective hard particle allowing for the presence of non-additive interactions between species in a binary mixture, and, most recently, introduction of a new model in which interactions between protein molecules are described in terms of a square well potential.

在各种组成和 pH 值、总蛋白浓度高达 35 g/l、核糖核酸酶带有净正电荷或负电荷，其他蛋白质带有净负电荷。 浓度依赖性在三个模型的背景下进行解释：统计热力学模型，有效硬颗粒的扩展，允许二元混合物中物种之间存在非加和相互作用，以及最近引入的新模型其中蛋白质分子之间的相互作用用方阱电势来描述。

项目成果

Beyond the second virial coefficient: Sedimentation equilibrium in highly non-ideal solutions.

超越第二维里系数：高度非理想溶液中的沉降平衡。

• 发表时间: 2011-05
• 作者: Rivas, Germán;Minton, Allen P
• 通讯作者: Minton, Allen P

Quantitative characterization of nonspecific self- and hetero-interactions of proteins in nonideal solutions via static light scattering.

通过静态光散射定量表征非理想溶液中蛋白质的非特异性自相互作用和异相互作用。

• 发表时间: 2015-02-05
• 作者: Wu, Di;Minton, Allen P
• 通讯作者: Minton, Allen P

Quantitative characterization of heparin binding to Tau protein: implication for inducer-mediated Tau filament formation.

肝素与 Tau 蛋白结合的定量表征：对诱导剂介导的 Tau 丝形成的影响。

• 发表时间: 2010-02-05
• 作者: Zhu, Hai;Fernández, Cristina;Fan, Jun;Shewmaker, Frank;Chen, Jie;Minton, Allen P;Liang, Yi
• 通讯作者: Liang, Yi

Quantitative characterization of the compensating effects of trimethylamine-N-oxide and guanidine hydrochloride on the dissociation of human cyanmethmoglobin.

三甲胺-N-氧化物和盐酸胍对人氰化高铁血红蛋白解离的补偿作用的定量表征。

• 发表时间: 2013-08-15
• 作者: Wu, Di;Minton, Allen P
• 通讯作者: Minton, Allen P

pH-dependent self-association of zinc-free insulin characterized by concentration-gradient static light scattering.

以浓度梯度静态光散射为特征的无锌胰岛素的 pH 依赖性自缔合。

• 发表时间: 2010-05
• 影响因子: 3.8
• 作者: Attri, Arun K;Fernández, Cristina;Minton, Allen P
• 通讯作者: Minton, Allen P