Properties of concentrated macromolecular solutions

高分子浓溶液的性质

• 批准号: 8741358
• 负责人: Allen P Minton
• 金额: $ 20.29万
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8741358
• 关键词: Accounting; Algorithms; Behavior; Cereals; Complex; Data Analyses; Dependence; Electrostatics; Equilibrium; Fibrinogen; Individual; Ionic Strengths; Liquid substance; Measurement; Measures; Methods; Modeling; Ovomucin; Property; Proteins; Radial; Reporting; Solutions; Techniques; Testing; Viscosity; macromolecule; novel; protein protein interaction; research study; simulation; theories

1. The viscometer/rheometer described in last year's report has been used to measure the concentration-dependent viscosity of three proteins - BSA, ovomucoid, and fibrinogen - individually, in binary mixtures, and in ternary mixtures. A semi-empirical relation requiring the specification of only two parameters per protein has been found to account quantitatively for the viscosity of individual proteins and mixtures at all concentrations up to 300 g/l to within experimental precision. (A. Grupi & A. Minton) 2. A compact yet extremely precise representation of the radial distribution function of hard sphere fluids and square well fluids has been discovered. This representation will be used to test semiempirical models of the colligative properties of such fluids (T. Hoppe) 3. A compact yet accurate method for coarse-graining electrostatic interactions between proteins has been developed. The novel coarse-graining algorithms will enable Monte Carlo and Brownian Dynamic simulations of the properties of concentrated proteins to be undertaken over a broad range of pH and ionic strength. (T. Hoppe)

1. 去年报告中描述的粘度计/流变仪已用于单独、二元混合物和三元混合物中测量三种蛋白质（BSA、卵类粘蛋白和纤维蛋白原）的浓度依赖性粘度。 已发现一种半经验关系，只需指定每种蛋白质的两个参数即可定量解释单个蛋白质和混合物在高达 300 g/l 的所有浓度下的粘度，并达到实验精度。 （A.格鲁皮和A.明顿） 2. 已经发现了硬球流体和方井流体的径向分布函数的紧凑而极其精确的表示。 该表示将用于测试此类流体的依数性质的半经验模型（T. Hoppe） 3. 已经开发出一种紧凑而准确的蛋白质间粗粒度静电相互作用方法。 新颖的粗粒度算法将使浓缩蛋白质的特性能够在广泛的 pH 和离子强度范围内进行蒙特卡罗和布朗动态模拟。 （T·霍普）