The Exercise and Physical Activity Collaborative Team (ExPACT): a Proposed MoTrPAC Clinical Center

锻炼和身体活动协作团队 (ExPACT)：拟建 MoTrPAC 临床中心

• 批准号: 10893749
• 负责人: Thomas W Buford
• 金额: $ 9.78万
• 依托单位: BALL STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-01 至 2023-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10893749
• 关键词: Acute; Aerobic; Alabama; Ancillary Study; Biological; Clinical; Clinical Trials; Collaborations; Collection; Coupled; Data; Deposition; Diabetes Mellitus; Disease; Enrollment; Ethnic Origin; Exercise; Florida; Future; Geography; Goals; Health; Health Benefit; Human; Information Dissemination; Knowledge; Laboratories; Leadership; Link; Manuscripts; Maps; Medicine; Metabolism; Methodology; Molecular; Outcome; Performance; Phase; Phenotype; Physical activity; Population; Procedures; Public Health; Quality Control; Race; Research Institute; Resistance; Rural; Seasons; Structure; Testing; Tissues; Training; Transducers; Translational Research; United States National Institutes of Health; Universities; Woman; Work; Writing; circadian; clinical center; clinical training; design; exercise training; experience; fitness; human tissue; improved; innovation; men; preservation; randomized trial; recruit; resistance exercise; response; sedentary; suburb

Project Summary The Molecular Transducers of Physical Activity Consortium (MoTrPAC) is designed to discover and characterize the range of molecular transducers (the ‘molecular map’) that underlie the effects of physical activity (PA) in humans. Our Exercise and Physical Activity Collaborative Team (ExPACT) –an alliance of the University of Alabama at Birmingham Center for Exercise Medicine (UAB), Translational Research Institute for Metabolism and Diabetes in Orlando, Florida (TRI-MD), and Ball State University Human Performance Laboratory (BSU) –has extensive experience performing highly-controlled aerobic (AT) and resistance (RT) exercise training clinical trials in healthy and diseased populations. The overarching goal of ExPACT is to contribute substantively to successful MoTrPAC planning, execution, and innovation. Phase 1 has already been completed and consisted of: Aim 1) Effectively collaborate with MoTrPAC leaders and NIH leadership to help guide and finalize the structure and standard operating procedures (SOPs). In the remaining years of the project, the ExPACT will implement and complete Phase II and Phase III, as follows: Phase II (Recruitment and Testing Years) Aim 1. We will enroll 420 sedentary but healthy men and women 18+ y into a rigorously controlled, 12-wk, randomized trial of AT (n=140), RT (n=140), or no-exercise control (CON, n=50). ExPACT geography will enrich MoTrPAC racial, ethnic, urban, suburban, and rural diversity. The design incorporates comprehensive phenotyping to assess fitness and clinical outcomes, and human tissue phenotyping to link the molecular transducers with key biological responses to exercise. Aim 2. Pre-and posttraining, we will obtain strategically timed biospecimens before and after acute exercise. To better understand the biological extremes, we will conduct the same acute response studies in highly-trained athletes (AT (n=25) or RT (n=25)). Serial biospecimen collection in CON will allow estimation of biological, seasonal, circadian and methodological variability. Aim 3. To enhance the overall scientific impact of MoTrPAC, we will continue to work with the consortium to conduct ancillary studies, which will markedly augment subject and tissue phenotyping and interpretation of the molecular maps. Phase III (Close-out) Aim 1. We will complete all ExPACT data entry, quality control, and central deposit procedures in collaboration with the MoTrPAC Coordinating Center, and we will actively contribute to the completion of biospecimen analyses, manuscript writing, and dissemination of results. We would be honored to collaborate with MoTrPAC to contribute future knowledge on the molecular mechanisms through which physical activity can improve or preserve health.

项目摘要 体育活动联盟（MOTRPAC）的分子换能器旨在发现 并表征了分子传感器（“分子图”）的范围 体育锻炼（PA）在人类中的影响。我们的运动和体育锻炼合作 团队（Expact） - 伯明翰锻炼中心阿拉巴马大学的联盟 医学（UAB），奥兰多代谢和糖尿病转化研究所， 佛罗里达州（TRI-MD）和Ball State University人类绩效实验室（BSU） - 进行高度控制的有氧（AT）和电阻（RT）练习的丰富经验 培训健康和疾病人群中的临床试验。外部的总体目标 是为成功的Motrpac计划，执行和创新做出实质性贡献。 第1阶段已经完成，包括：目标1）有效合作 MOTRPAC领导者和NIH领导层帮助指导和最终确定结构和标准 操作程序（SOP）。在该项目的剩余几年中，外籍将实施 以及II阶段和第三阶段的完成，如下：II期（招聘和测试年）目标 1。我们将注册420个久坐但健康的男人和女性18岁以上的健康， 12-WK，AT（n = 140），RT（n = 140）或无运动控制（con，n = 50）的随机试验。 外界地理将丰富Motrpac种族，种族，城市，郊区和粗糙的多样性。 该设计结合了全面的表型，以评估适应性和临床结果， 和人体组织表型将分子传感器与关键生物学反应联系起来 锻炼。 AIM 2。训练前和训练，我们将在战略上获得定时的生物测量 并在急性运动之后。为了更好地理解生物学的极端，我们将进行 在训练有素的运动员（n = 25）或RT（n = 25）中相同的急性反应研究。系列 CON中的生物循环收集将允许估计生物学，季节性，昼夜节律和 方法上的变异性。目标3。为了增强Motrpac的整体科学影响，我们将 继续与财团合作进行辅助研究，这将明显 增强受试者和组织表型和分子图的解释。第三阶段 （结束）目标1。我们将完成所有Excact数据输入，质量控制和中央存款 与MOTRPAC协调中心合作的程序，我们将积极地 有助于完成生物测量分析，手稿写作和传播 结果。我们很荣幸与Motrpac合作，以贡献有关未来的知识 体育活动可以改善或保留健康的分子机制。

项目成果

Human adipose and skeletal muscle tissue DNA, RNA, and protein content.

人体脂肪和骨骼肌组织 DNA、RNA 和蛋白质含量。

• DOI: 10.1152/japplphysiol.00343.2021
• 发表时间: 2021
• 期刊: Journal of applied physiology (Bethesda, Md. : 1985)
• 作者: Stroh,AndrewM;Lynch,ColleenE;Lester,BridgetE;Minchev,Kiril;Chambers,TobyL;Montenegro,CristhianF;ChavezMartinez,Clarisa;Fountain,WilliamA;Trappe,ToddA;Trappe,ScottW
• 通讯作者: Trappe,ScottW

Isolation of nuclei from frozen human subcutaneous adipose tissue for full-length single-nuclei transcriptional profiling.

• DOI: 10.1016/j.xpro.2023.102054
• 发表时间: 2023-03-17
• 期刊: STAR PROTOCOLS
• 作者: Whytock, Katie L.;Divoux, Adeline;Sun, Yifei;Hopf, Meghan;Yeo, Reichelle X.;Pino, Maria F.;Yu, Gongxin;Smith, Steven R.;Walsh, Martin J.;Sparks, Lauren M.
• 通讯作者: Sparks, Lauren M.