DGRC Resource Core

DGRC 资源核心

• 批准号: 10839522
• 负责人: Andrew Zelhof
• 金额: $ 23.5万
• 依托单位: TRUSTEES OF INDIANA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10839522
• 关键词: Anterior; Applied Research; Archives; Behavior; Biological Models; Biological Process; Biomedical Research; CRISPR/Cas technology; Cell Cycle; Cell Line; Cells; Cessation of life; Chromatin Modeling; Collaborations; Communities; Complementary DNA; Coupled; Data Management Resources; Development; Disease; Drosophila genome; Drosophila genus; Drosophila melanogaster; Epigenetic Process; Erinaceidae; Evolution; FlyBase; Funding Agency; Gene Expression Regulation; Genes; Genetic; Genome; Goals; Grant; Homeostasis; Homologous Gene; Human; Individual; International; Malignant Neoplasms; Meiosis; Metabolic; Mission; Mitosis; Modeling; Nerve Degeneration; Neurobiology; Pathway interactions; Pattern; Physiological Processes; Plasmid Cloning Vector; Population; Process; Protocols documentation; RNA Interference; Receptor Protein-Tyrosine Kinases; Recovery; Reproducibility; Research; Resources; Scientist; Signal Pathway; Signal Transduction; Specific qualifier value; Surveys; Synapses; Tissues; Transfection; United States National Institutes of Health; Update; Work; aerobic glycolysis; biomedical resource; cost; fundamental research; gene conservation; genome resource; human disease; improved; neural; neuroblast; notch protein; preservation; programs; repository; sensor; stem cell biology; stem cell model; stem cells; success; technology development; vector; web interface

Project Summary: The Drosophila Genomics Resource Center (DGRC) supports the international community of scientists utilizing Drosophila melanogaster for biomedical research. The mission of the DGRC is to 1) provide broad access to genomics resources by acquiring, archiving, curating, and distributing genomics resources including clones, vectors, and cell lines; 2) facilitate effective use of these genomics resources by providing guidance and support; and 3) improve the genomics resources and protocols available for Drosophila research. By preserving vital research materials and distributing them efficiently, the DGRC assures economical access and enhances scientific rigor and reproducibility. The first aim of this proposal is to continue and strengthen the successful DGRC programs for acquiring, distributing genomics resources and facilitating their effective use. This will include augmenting and updating data management systems, the web interface and user support, as well as continuing the effective cost recovery program. The goal is to maximize the long-term viability of the DGRC and its benefits to users and the NIH. The second aim is to increase the utility of Drosophila as a model system by generating new resources through four projects: 1) surveying the transfection and CRISPR/Cas9 efficiency of gene tagging across modENCODE cell lines, 2) generating a universal CRISPR/Cas9 based transfection toolkit for the insertion of constructs and the subsequent ability to generate stable transformants in any Drosophila cell line, 3) establishing a neuroblast cell line as a model for stem cell biology, and 4) creating new metabolic sensors for characterizing physiological processes in cell lines and Drosophila tissues.

项目摘要： 果蝇基因组资源中心（DGRC）支持国际社会 科学家利用果蝇Melanogaster进行生物医学研究。任务 DGRC是1）通过获取，归档，策展，可提供对基因组学资源的广泛访问权限 并分发基因组学资源，包括克隆，向量和细胞系； 2）便利 通过提供指导和支持来有效利用这些基因组学资源； 3） 改善可用于果蝇研究的基因组资源和方案。经过 DGRC保留重要的研究材料并有效地分配它们 经济访问并增强了科学严格和可重复性。第一个目的 建议将继续并加强成功获取的DGRC计划， 分发基因组学资源并促进其有效使用。这将包括 增强和更新数据管理系统，Web界面和用户支持， 以及继续有效的成本恢复计划。目标是最大化长期 DGRC的生存能力及其对用户和NIH的好处。第二个目标是增加 果蝇作为模型系统的实用程序，通过四个项目生成新资源：1） 测量基因标记的转染和CRISPR/CAS9跨度 Modencode Cell系列，2）生成基于通用CRISPR/CAS9的转染工具包 为了插入构建体以及随后产生稳定转化体的能力 任何果蝇细胞系，3）建立神经细胞细胞系作为干细胞的模型 生物学，以及4）创建新的代谢传感器来表征生理过程 细胞系和果蝇组织。