DROSOPHILA GENOMICS RESOURCE CENTER

果蝇基因组资源中心

基本信息

批准号：
10816787
负责人：
Andrew Zelhof
金额：
$ 23.5万
依托单位：
TRUSTEES OF INDIANA UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-04-01 至 2024-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10816787
关键词：
Administrative Supplement Applied Research Archives Biocompatible Materials Biological Models Biological Process Biomedical Research CRISPR/Cas technology Cell Line Cells Collaborations Collection Communities DNA Data Management Resources Databases Drosophila genus Drosophila melanogaster FlyBase Genes Goals International Metabolic Mission Molecular Online Systems Pathway interactions Physiological Processes Protocols documentation Public Health Recovery Reproducibility Research Resources Scientist Surveys Tissues Transfection United States National Institutes of Health Update aerobic glycolysis biomedical resource cost fundamental research genome resource human disease improved model organism neuroblast parent grant preservation programs repository sensor stem cell biology stem cell model stem cells vector web interface

项目摘要

Project Summary: The Drosophila Genomics Resource Center (DGRC) supports the international community of scientists utilizing Drosophila melanogaster for biomedical research. The mission of the DGRC is to 1) provide broad access to genomics resources by acquiring, archiving, curating, and distributing genomics resources including clones, vectors, and cell lines; 2) facilitate effective use of these genomics resources by providing guidance and support; and 3) improve the genomics resources and protocols available for Drosophila research. By preserving vital research materials and distributing them efficiently, the DGRC assures economical access and enhances scientific rigor and reproducibility. The first aim of this proposal is to continue and strengthen the successful DGRC programs for acquiring, distributing genomics resources and facilitating their effective use. This will include augmenting and updating data management systems, the web interface and user support, as well as continuing the effective cost recovery program. The goal is to maximize the long-term viability of the DGRC and its benefits to users and the NIH. The second aim is to increase the utility of Drosophila as a model system by generating new resources through four projects: 1) surveying the transfection and CRISPR/Cas9 efficiency of gene tagging across modENCODE cell lines, 2) generating a universal CRISPR/Cas9 based transfection toolkit for the insertion of constructs and the subsequent ability to generate stable transformants in any Drosophila cell line, 3) establishing a neuroblast cell line as a model for stem cell biology, and 4) creating new metabolic sensors for characterizing physiological processes in cell lines and Drosophila tissues.

项目概要：果蝇基因组学资源中心 (DGRC) 支持国际利用黑腹果蝇进行生物医学研究的科学家群体。这 DGRC 的使命是 1) 通过获取、归档、管理和分发基因组学资源，包括克隆、载体和细胞系； 2）通过提供指导促进这些基因组学资源的有效利用和支持； 3）改进可用的基因组学资源和协议果蝇研究。通过保存重要的研究材料并分发它们 DGRC 有效地确保了经济的获取并增强了科学严谨性和再现性。该提案的首要目标是继续并加强成功的 DGRC 旨在获取、分发基因组学资源并促进其发展的计划有效利用。这将包括增强和更新数据管理系统、网络界面和用户支持，以及继续有效的成本回收程序。目标是最大限度地提高 DGRC 的长期生存能力及其对用户和 NIH。第二个目标是提高果蝇作为模型的实用性通过四个项目生成新资源来构建系统：1）调查转染以及 modENCODE 细胞系中基因标记的 CRISPR/Cas9 效率，2) 生成基于 CRISPR/Cas9 的通用转染工具包，用于插入构建体以及随后在任何果蝇中产生稳定转化体的能力细胞系，3) 建立神经母细胞系作为干细胞生物学模型，以及 4) 创建新的代谢传感器来表征细胞系的生理过程和果蝇组织。