DGRC Resource Core

DGRC 资源核心

基本信息

批准号：
10385738
负责人：
Andrew Zelhof
金额：
$ 48.33万
依托单位：
TRUSTEES OF INDIANA UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
起止时间：
2002-04-01 至 2024-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10385738
关键词：
Anterior Applied Research Archives Behavior Biological Models Biological Process Biomedical Research CRISPR/Cas technology Cell Cycle Cell Line Cells Cessation of life Chromatin Modeling Cloning Vectors Communities Complementary DNA Coupled Data Management Resources Development Disease Drosophila genome Drosophila genus Drosophila melanogaster Epigenetic Process Equilibrium Erinaceidae Evolution FlyBase Funding Agency Gene Expression Regulation Genes Genetic Genome Genomics Goals Grant Homeostasis Homologous Gene Human Individual International Malignant Neoplasms Meiosis Metabolic Mission Mitosis Modeling Nerve Degeneration Neurobiology Pathway interactions Pattern Physiological Processes Plasmid Cloning Vector Population Process Protocols documentation RNA Interference Receptor Protein-Tyrosine Kinases Recovery Reproducibility Research Resources Scientist Ships Signal Pathway Signal Transduction Surveys Synapses Tissues Transfection Transgenic Organisms United States National Institutes of Health Update Work aerobic glycolysis base biomedical resource bone morphogenetic protein receptors cost fundamental research human disease improved neuroblast notch protein preservation programs relating to nervous system repository sensor stem cell biology stem cell model stem cells success vector web interface

项目摘要

Project Summary: The Drosophila Genomics Resource Center (DGRC) supports the international community of scientists utilizing Drosophila melanogaster for biomedical research. The mission of the DGRC is to 1) provide broad access to genomics resources by acquiring, archiving, curating, and distributing genomics resources including clones, vectors, and cell lines; 2) facilitate effective use of these genomics resources by providing guidance and support; and 3) improve the genomics resources and protocols available for Drosophila research. By preserving vital research materials and distributing them efficiently, the DGRC assures economical access and enhances scientific rigor and reproducibility. The first aim of this proposal is to continue and strengthen the successful DGRC programs for acquiring, distributing genomics resources and facilitating their effective use. This will include augmenting and updating data management systems, the web interface and user support, as well as continuing the effective cost recovery program. The goal is to maximize the long-term viability of the DGRC and its benefits to users and the NIH. The second aim is to increase the utility of Drosophila as a model system by generating new resources through four projects: 1) surveying the transfection and CRISPR/Cas9 efficiency of gene tagging across modENCODE cell lines, 2) generating a universal CRISPR/Cas9 based transfection toolkit for the insertion of constructs and the subsequent ability to generate stable transformants in any Drosophila cell line, 3) establishing a neuroblast cell line as a model for stem cell biology, and 4) creating new metabolic sensors for characterizing physiological processes in cell lines and Drosophila tissues.

项目概要：果蝇基因组资源中心 (DGRC) 支持国际社会科学家利用黑腹果蝇进行生物医学研究。的使命 DGRC 的目标是 1) 通过获取、存档、整理、提供对基因组学资源的广泛访问分发基因组学资源，包括克隆、载体和细胞系； 2）促进通过提供指导和支持有效利用这些基因组学资源；和 3) 改善可用于果蝇研究的基因组学资源和方案。经过 DGRC 保证保存重要的研究材料并有效地分发它们经济地获取并提高科学严谨性和可重复性。本次活动的第一个目的建议继续并加强 DGRC 成功的计划，以获取、分配基因组学资源并促进其有效利用。这将包括增强和更新数据管理系统、网络界面和用户支持，以及继续有效的成本回收计划。目标是最大化长期利益 DGRC 的可行性及其对用户和 NIH 的好处。第二个目标是增加通过四个项目产生新资源，利用果蝇作为模型系统：1) 调查基因标签的转染和 CRISPR/Cas9 效率 modENCODE 细胞系，2) 生成基于 CRISPR/Cas9 的通用转染工具包用于构建体的插入和随后产生稳定转化体的能力任何果蝇细胞系，3) 建立神经母细胞系作为干细胞模型生物学，4）创建新的代谢传感器来表征生理过程细胞系和果蝇组织。