NCCAT: National Center for CryoEM Access and Training--Screening Supplement

NCCAT：国家冷冻电镜访问和培训中心 - 筛选补充

• 批准号: 10830733
• 负责人: Jeffrey S Kieft
• 金额: $ 27.93万
• 依托单位: NEW YORK STRUCTURAL BIOLOGY CENTER
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-05-01 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10830733
• 关键词: Cryoelectron Microscopy; Dedications; Ensure; Equipment; Funding; Goals; Image; Macromolecular Complexes; Methods; Microscope; Modeling; New York; Preparation; Procedures; Production; Qualifying; Resolution; Resources; Robot; Sampling; Services; Specimen; Standardization; Structure; Support Groups; Training; Training Programs; Transmission Electron Microscopy; biological systems; career; detector; experience; particle; screening; screening services; skills; structural biology; supplemental screening

Project Summary/Abstract The National Center for CryoEM Access and Training (NCCAT), based at the New York Structural Biology Center, provides access to state-of-the-art cryo-electron microscopy (cryoEM) equipment. The equipment and services include specimen preparation robots, high-end microscopes with direct detectors, screening microscopes, and other ancillary equipment required to solve structures to the highest possible resolution using cryoEM methods. A highly qualified technical team provides direct support, guidance, and assistance, ensuring the highest quality structures are resolved quickly and efficiently. A cross-training program offers training to users across the full spectrum of skill levels and career goals. This is a request for supplemental funding to support a grid preparation and screening (GPS) service for cryo-transmission electron microscopy (cryoTEM) at NCCAT. The objective is to provide urgently needed support to groups with limited experience in cryoEM methods and limited access to local facilities. The goals will be to support these groups and get them started, while also training them in all aspects of the methods so they can make the best use of every resource available to them as soon as possible.

项目摘要/摘要 总部位于纽约结构的国家冷冻访问与培训中心（NCCAT） 生物学中心，提供了最先进的冷冻电子显微镜（冷冻）设备的访问权限。这 设备和服务包括标本准备机器人，直接的高端显微镜 探测器，筛选显微镜和其他辅助设备解决结构 使用冷冻方法的最高分辨率。一个高素质的技术团队提供直接 支持，指导和协助，确保迅速解决最高质量的结构 有效。一个交叉培训计划为各种技能水平的用户提供培训， 职业目标。 这是提供补充资金以支持电网准备和筛选（GPS）服务的请求 用于NCCAT的冷冻传输电子显微镜（冰冻）。目的是紧急提供 需要在冷冻方法经验有限且访问本地经验有限的团体中需要支持 设施。目标是支持这些小组并使他们开始，同时也培训他们 方法的各个方面，以便它们可以充分利用每种资源，以便尽快使用 可能的。

项目成果

Conformational switching and flexibility in cobalamin-dependent methionine synthase studied by small-angle X-ray scattering and cryoelectron microscopy.

• DOI: 10.1073/pnas.2302531120
• 发表时间: 2023-06-27
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Watkins, Maxwell B.;Wang, Haoyue;Burnim, Audrey;Ando, Nozomi
• 通讯作者: Ando, Nozomi

Structure of the human cation-chloride cotransport KCC1 in an outward-open state.

• DOI: 10.1073/pnas.2109083119
• 发表时间: 2022-07-05
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1

Absolute structure determination of Berkecoumarin by X-ray and electron diffraction.

通过 X 射线和电子衍射测定 Berkecoumarin 的绝对结构。

• DOI: 10.1107/s2053229624003061
• 发表时间: 2024
• 期刊: Acta crystallographica. Section C, Structural chemistry
• 作者: Decato,Daniel;Palatinus,Lukáš;Stierle,Andrea;Stierle,Donald
• 通讯作者: Stierle,Donald

Structural diversity of the SARS-CoV-2 Omicron spike.

• DOI: 10.1016/j.molcel.2022.03.028
• 发表时间: 2022-06-02
• 期刊: Molecular cell
• 影响因子: 16
• 作者: Gobeil SM;Henderson R;Stalls V;Janowska K;Huang X;May A;Speakman M;Beaudoin E;Manne K;Li D;Parks R;Barr M;Deyton M;Martin M;Mansouri K;Edwards RJ;Eaton A;Montefiori DC;Sempowski GD;Saunders KO;Wiehe K;Williams W;Korber B;Haynes BF;Acharya P
• 通讯作者: Acharya P

Structural polymorphism of amyloid fibrils in ATTR amyloidosis revealed by cryo-electron microscopy.

• DOI: 10.1038/s41467-024-44820-3
• 发表时间: 2024-01-17
• 期刊: Nature communications
• 影响因子: 16.6
• 作者: Nguyen BA;Singh V;Afrin S;Yakubovska A;Wang L;Ahmed Y;Pedretti R;Fernandez-Ramirez MDC;Singh P;Pękała M;Cabrera Hernandez LO;Kumar S;Lemoff A;Gonzalez-Prieto R;Sawaya MR;Eisenberg DS;Benson MD;Saelices L
• 通讯作者: Saelices L