Cellular Imaging Core

细胞成像核心

• 批准号: 10704277
• 负责人: David W Piston
• 金额: $ 11.66万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-01 至 2028-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10704277
• 关键词: 3-Dimensional; Acceleration; Animals; Area; Basic Science; Biological Assay; Biological Models; Cell physiology; Cells; Charge; Complement; Consultations; Contrast Media; Cryoelectron Microscopy; Custom; Data Analyses; Data Set; Dedications; Development; Dimensions; Disease; Education; Educational workshop; Electron Microscopy; Electrons; Ensure; Equipment; Experimental Designs; Faculty; Feedback; Fluorescence; Funding; Generations; Goals; Grant; Human Resources; Image; Image Analysis; Imaging Techniques; Imaging technology; Individual; Informatics; Institution; Investments; Ions; Joints; Laboratories; Laboratory Research; Learning; Light; Macromolecular Complexes; Methodology; Microscope; Microscopic; Microscopy; Optics; Organ; Pathogenesis; Pathway interactions; Play; Preparation; Process; Proteins; Research; Research Personnel; Resolution; Resources; Rheumatism; Roentgen Rays; Role; Sampling; Scholarship; Services; Specialist; Specimen; Speed; Structure; System; Technical Expertise; Techniques; Technology; Time; Tissues; Training; Translating; Translational Research; Universities; Visualization; Washington; Work; X ray microscopy; biological research; bone; cell behavior; cell type; cellular imaging; cost; data acquisition; imaging facilities; imaging study; improved; innovation; instrumentation; light microscopy; live cell imaging; member; microscopic imaging; model organism; molecular imaging; novel; operation; programs; quantitative imaging; research study; response; single molecule; superresolution imaging; superresolution microscopy; technology platform; tissue fixing; tool; translational study; two-dimensional; two-photon

PROJECT SUMMARY: Cellular Imaging Core Advanced cellular microscopy is a powerful tool for biological research and has an important role to play in the study of disease pathogenesis, which may translate eventually to novel treatment approaches for rheumatic diseases. Imaging technology has evolved rapidly over the last decade leading to improvements in resolution, sensitivity and speed which have created fundamentally new opportunities for studying processes across many orders of magnitude and in real-time in living cells and animals. At the same time, the costs of increasingly sophisticated equipment are substantial and the expertise to efficiently use, maintain, and develop this equipment is not common in most labs. There is therefore a significant gap between the availability of these powerful tools and the ability of investigators to access and use them efficiently. The Cellular Imaging Core (CIC) was originally created in response to feedback from Washington University Rheumatic Diseases Research Resource-based Center (WU-RDRRC) members to leverage the significant institutional investment in the newly created Washington University Center for Cellular Imaging (WUCCI) and provide an integrated approach to investigate the structure and dynamic behavior of cells and tissues in rheumatic disease-related model systems. The overall objective of the core is to provide access to and technical support in using advanced cellular microscopy tools to accelerate the pace, expand the scope, and improve efficiency of rheumatic diseases research. The core services meet the unique requirements of numerous investigators over a wide range of basic and translational research, attracting new investigators into rheumatic diseases research areas. Importantly the users benefit from the in-depth technical expertise of the Core Director and technical staff in the time spent in consultation for experimental design and interpretation of data. During the past funding cycle, the core served 28 WU-RDRRC investigator research laboratories, who expensed $520,699 in core recharges, which represented approximately 12% of all WUCCI activity. Fourteen WU-RDRRC investigator laboratories received micro-grant funding through the WU- RDRRC Imaging Core Scholarship program, 5 of which were junior faculty members. Given the broad usage of core over the past funding cycle, and the establishment of new imaging technologies (e.g. cryoEM and lattice lightsheet), sample preparation services (e.g. tissue clearing), and analysis approaches (e.g. AI-based IHC and IF image analysis), it is anticipated that requests for CIC services will continue to be robust given the overwhelming need for advanced microscopic imaging and image analysis approaches, thus accelerating the research endeavors of WU-RDRRC members throughout the next funding cycle.

项目摘要：蜂窝成像核心 高级蜂窝显微镜是生物学研究的强大工具，在 疾病发病机理的研究可能最终转化为新的治疗方法 风湿病。在过去的十年中，成像技术已经迅速发展，从而改善了 在解决方案，敏感性和速度方面为研究创造了新的新机会 在活细胞和动物中，许多数量级和实时的过程中的过程。同时， 越来越复杂的设备的成本是巨大的，并且有效地使用，维护， 在大多数实验室中，开发此设备并不常见。因此， 这些强大的工具的可用性以及调查人员有效访问和使用它们的能力。这 蜂窝成像核心（CIC）最初是为了响应华盛顿大学的反馈而创建的 风湿病基于研究资源的中心（WU-RDRRC）成员利用重要的 在新成立的华盛顿大学蜂窝成像中心（WUCCI）和 提供一种集成的方法来研究细胞和组织中细胞和组织的结构和动态行为 风湿性疾病相关模型系统。核心的总体目的是提供对和 使用先进的蜂窝显微镜工具来加速步伐，扩展范围，并 提高风湿病研究的效率。核心服务符合独特的要求 许多研究人员在广泛的基础和转化研究中吸引了新的研究者 进入风湿病研究领域。重要的是，用户从深入的技术专长中受益 在咨询实验设计和 数据解释。在过去的融资周期中，核心服务28 WU-RDRRC研究者研究 实验室，支出了520,699美元的核心充值，约占所有Wucci的12％ 活动。 14个WU-RDRRC研究者实验室通过Wu-获得了微赠品资金 RDRRC成像核心奖学金计划，其中5个是初级教师。鉴于广泛的用法 核心在过去的融资周期中，以及建立新成像技术（例如冷冻和 晶格灯表），样品制备服务（例如组织清除）和分析方法（例如基于AI的 IHC和如果图像分析），则预计CIC服务的请求将继续很强 对高级显微成像和图像分析方法的压倒性需求，从而加速了 在下一个融资周期中，WU-RDRRC成员的研究努力。