Nikon Confocal Microscope for Shared Biomedical Research

用于共享生物医学研究的尼康共焦显微镜

• 批准号: 10413403
• 负责人: David W Piston
• 金额: $ 53.76万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-05-01 至 2023-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10413403
• 关键词: 3-Dimensional; Applications Grants; Basic Science; Biological Models; Biomedical Research; Cell physiology; Cells; Cellular biology; Diabetes Mellitus; Ensure; Functional Imaging; Funding; Head; Homeostasis; Image; Immersion; Institution; Lasers; Leadership; Metabolic; Microscope; Microscopy; Minor; Monitor; Neurodegenerative Disorders; Pathogenesis; Physiology; Research; Research Personnel; Resources; S10 grant; Scanning; Specimen; System; Technology; Time; Tissues; Training Support; Translational Research; United States National Institutes of Health; Universities; Viral; Visualization; Washington; Work; base; cell motility; cellular imaging; circadian; cost efficient; detector; experience; imaging platform; imaging system; instrument; lens; medical schools; mitochondrial dysfunction; programs; research study; success; 3-Dimensional; Applications Grants; Basic Science; Biological Models; Biomedical Research; Cell physiology; Cells; Cellular biology; Diabetes Mellitus; Ensure; Functional Imaging; Funding; Head; Homeostasis; Image; Immersion; Institution; Lasers; Leadership; Metabolic; Microscope; Microscopy; Minor; Monitor; Neurodegenerative Disorders; Pathogenesis; Physiology; Research; Research Personnel; Resources; S10 grant; Scanning; Specimen; System; Technology; Time; Tissues; Training Support; Translational Research; United States National Institutes of Health; Universities; Viral; Visualization; Washington; Work; base; cell motility; cellular imaging; circadian; cost efficient; detector; experience; imaging platform; imaging system; instrument; lens; medical schools; mitochondrial dysfunction; programs; research study; success

Project Summary/Abstract This proposal represents a request from a group of 15 NIH-funded investigators with overlapping imaging needs for funding to acquire a Nikon AX Laser Scanning Confocal Microscope (LSM) to facilitate the visualization and quantification of the three-dimensional spatial organization of fixed cells and tissues as well as living specimens, with the latter including the need to monitor time-dependent changes. The system will be housed in the Washington University Center for Cellular Imaging (WUCCI), an institution-wide shared technology resource based at the School of Medicine. The current laser scanning confocal microscopes in the WUCCI are already heavily subscribed by different user groups with different needs (upright configuration for model system imaging and Airyscan capabilities for sub-diffraction limited imaging) and additional workhorse confocal capacity is needed to facilitate the work of the Major and Minor user projects described herein. The Nikon AX confocal microscope platform is configured with (i) a Nikon Ti2 inverted motorized microscope frame, (ii) 405 nm, 488 nm, 561 nm, and 640 nm excitation lasers, (iii) high-sensitivity filter-based and spectral detectors, (iv) a variety of dry and immersion-based objective lenses and (v) a stage-top incubation system to facilitate the physiological imaging of living specimens. Fifteen investigators from nine different units at the School of Medicine will make use of this imaging platform to enable a wide-range of basic and translational research studies aimed at understanding the mechanisms of viral and bacterial pathogenesis, the dynamics of cell migration, mitochondrial dysfunction, metabolic homeostasis, diabetes, circadian physiology and neurodegenerative disease. While the instrument has been configured to meet the specific needs of the major user group, we thoroughly expect it to impact many other research programs. The expertise and institutional support for this instrument are exceptional. Dr. James Fitzpatrick, the Scientific Director of WUCCI, and Dr. David Piston, the Chair of Cell Biology & Physiology and head of the WUCCI Advisory Board, are both world-renowned experts in cellular microscopy and their combined leadership brings over 40 years of experience in providing cost-efficient training and support for high quality quantitative cellular imaging to a wide range of NIH-funded users. In support of this S10 grant application, the institution will also commit $100,000 ($20,000 per year for five years) to ensure the long-term success of this instrument.

项目摘要/摘要 该提案代表了一组15个由NIH资助的调查员的请求，这些调查员有重叠的成像需求 为了获得尼康斧激光扫描共聚焦显微镜（LSM）的资金，以促进可视化和 定量固定细胞和组织的三维空间组织以及生物标本 后者包括需要监视时间相关的变化。该系统将安置在 华盛顿大学蜂窝成像中心（WUCCI），一个机构范围的共享技术资源 位于医学院。 WUCCI中的当前激光扫描共聚焦显微镜已经是 由不同需求的不同用户组大量订阅（模型系统成像的直立配置 以及针对子分量有限成像的发电机功能）和其他主力共聚焦容量是 需要促进此处描述的主要和次要用户项目的工作。尼康斧头共焦 显微镜平台配置了（i）尼康Ti2倒电动显微镜框架，（ii）405 nm，488 nm， 561 nm和640 nm激发激光器，（iii）高敏化过滤器和光谱探测器，（iv）多种干燥 以及基于沉浸式的目标镜头和（v）阶段顶孵育系统，以促进生理 生物标本的成像。来自医学院的九个不同单位的15名调查员将使 使用此成像平台来实现旨在的大量基本和转化研究 了解病毒和细菌发病机理的机制，细胞迁移的动力学，线粒体的动力学 功能障碍，代谢稳态，糖尿病，昼夜节律生理学和神经退行性疾病。而 仪器已被配置为满足主要用户组的特定需求，我们完全期望它 影响许多其他研究计划。对该工具的专业知识和机构支持非常出色。 WUCCI的科学总监James Fitzpatrick博士和Cell Biology＆David Piston博士 生理学和WUCCI顾问委员会负责人都是世界知名的细胞显微镜专家 他们的联合领导才带来超过40年的经验，以提供成本效益的培训和支持 高质量的定量蜂窝成像，可用于广泛的NIH资助用户。支持这份S10赠款 申请，该机构还将承诺100,000美元（五年$ 20,000），以确保长期 该乐器的成功。