Johns Hopkins Human Temporal Bone Resource

约翰霍普金斯大学人类颞骨资源

• 批准号: 10705769
• 负责人: John Patrick Carey
• 金额: $ 79.24万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-16 至 2027-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10705769
• 关键词: 2019-nCoV; 3-Dimensional; Academic Medical Centers; Acceleration; Address; Adopted; Affect; African American population; Age; Aging; Animal Disease Models; Arachnoid mater; Auditory; Autopsy; Baltimore; Biological; Brain; Brain Stem; Catalogs; Childhood; Clinical; Cochlea; Collaborations; Collection; Communication; Communities; Consent; Data; Databases; Decalcification; Democracy; Disease; Ear; Ear Diseases; Education; Education and Outreach; Educational Materials; Enrollment; Ensure; Equilibrium; Equipment; Facial nerve structure; Faculty; Funding; Goals; Harvest; Health; Hearing; Hearing problem; Hematoxylin and Eosin Staining Method; Histology; Human; Image; Improve Access; Information Dissemination; Infrastructure; Intention; Knowledge; Laboratories; Laboratory Finding; Laboratory Research; Labyrinth; Longitudinal Studies; Measures; Medical; Medical Illustration; Melanins; Meniere' s Disease; Mentors; Modernization; Nature; Neurosciences; Online Systems; Otology; Participant; Pathology; Pattern; Persons; Population Heterogeneity; Population Study; Postdoctoral Fellow; Presbycusis; Prevalence; Process; Proliferating; Protocols documentation; Recording of previous events; Research; Research Personnel; Resources; Role; Scientist; Semicircular canal structure; Sickle Cell Anemia; Slide; Specimen; Stains; Students; Techniques; Temporal bone structure; Testing; Time; Tissues; Training; Uncertainty; Underrepresented Populations; Underrepresented Students; Visualization; Work; analysis pipeline; bilingualism; cost; crowdsourcing; demographics; digital; education resources; epidemiology study; equilibration disorder; imaging platform; inner ear diseases; macula; meetings; microCT; nerve supply; nonhuman primate; novel; otoconia; outreach; pre-doctoral; preservation; programs; rapid technique; reconstruction; recruit; response; sex; timeline; tool; training opportunity; transcriptomics; working group

Project Summary Temporal bone research is essential for refining our understanding of the mechanisms of otologic disease; however, the difficulty in accessing this tissue, the typically long processing times, and the expense have contributed to a dwindling number of labs engaged in this work. In the 1920s, the first human temporal bone laboratory in the U.S. was established at Johns Hopkins. The collection contains approximately ~1400 processed specimens from donors with a range of otologic diseases that were obtained primarily in the 1920s through 1970s, but only a handful of studies have made use of this collection in the past 20 years. We propose to make the collection more accessible to the scientific community and to re-establish the infrastructure for harvesting, imaging, and processing new specimens with the intention of recruiting new donors from population-based studies of aging in which participants have already consented to autopsies and brain donations being performed at Johns Hopkins, as well as other clinical otological studies ongoing in the Baltimore Metro Region. We will partner with faculty specializing in biological visualization, bilingual scientific communication, and mentoring diverse scientists to develop inclusive outreach tools. We will also partner with Baltimore-based transcriptomics resources to establish protocols for performing spatial transcriptomics analysis in nonhuman primate and human temporal bones. We will approach these objectives with three Specific Aims: 1) Catalog, prioritize, and digitize existing specimens from the Johns Hopkins Human Temporal Bone Collection; 2) Develop efficient harvesting, rapid histology, and spatial transcriptomics workflows for new specimens and establish partnerships for collecting specimens during autopsies performed on participants in epidemiological studies; 3) Education/outreach activities to encourage other investigators to engage in temporal bone research. The large temporal bone collection and the inner ear illustrations of Max Brödel at Johns Hopkins contributed greatly to our early understanding of many diseases of the ear, such as the discovery of glomus bodies, the histopathologic correlates of presbycusis, and innervation of the saccular maculae. The collection has also shown enduring value, contributing to our understanding of the prevalence of a dehiscent superior semicircular canal and arachnoid granulations as well as the role of melanin in cochlear and vestibular protection. Sharing this valuable collection with other researchers while applying novel techniques in digital processing and education will accelerate advances in and knowledge of otopathology. We look forward to reinvigorating this tradition of excellence by developing a new collection, applying modern techniques, and integrating otopathology with population-based studies that include comprehensive otologic testing and functional measures.

项目摘要 颞骨研究对于完善我们对耳科疾病机制的理解至关重要。 但是，很难进入该组织，通常的处理时间和费用很长 助长了从事这项工作的大量实验室。在1920年代，第一个人类临时骨头 美国的实验室是在约翰·霍普金斯（Johns Hopkins）成立的。该集合包含大约〜1400个过程 来自具有一系列耳科疾病的捐助者的标本，主要是在1920年代通过 1970年代，但在过去20年中，只有少数研究利用了该系列。我们建议做 该集合更容易被科学界访问，并重新建立收获基础设施， 成像和处理新规格，目的是招募基于人群的新捐助者 参与者已经同意进行尸检和大脑捐赠的衰老研究 在约翰·霍普金斯（Johns Hopkins）以及巴尔的摩都会区正在进行的其他临床耳效研究。我们将 与专门从事生物可视化，双语科学交流和心理化的教师合作 潜水员科学家开发包容性外展工具。我们还将与基于巴尔的摩的转录组学合作 建立在非人类灵长类动物和人类中执行空间转录组学分析协议的资源 临时骨头。我们将以三个特定的目的实现这些目标：1）目录，优先和数字化 来自约翰·霍普金斯人类颞骨收集的现有标本； 2）产生有效的收获， 快速的组织学和新标本的空间转录组学工作流，并建立合作伙伴关系 在流行病学研究参与者进行尸检期间的标本； 3）教育/外展 鼓励其他研究人员进行临时骨研究的活动。 约翰·霍普金斯（Johns Hopkins）的大型临时骨头收集和MaxBrödel的内耳插图贡献了 大大了解了我们对许多耳朵疾病的早期理解，例如发现Glomus身体， 长期扫描的组织病理学相关性和糖果斑疹神经的神经。该系列也有 显示了最终值，有助于我们理解去感染超级圆的流行率 运河和蛛网膜颗粒以及黑色素在人工耳蜗和前庭保护中的作用。分享 在数字处理中运用新颖技术的同时，与其他研究人员的这一有价值的收藏 教育将加速前进和知识。我们期待重新激发这个 通过开发新系列，应用现代技术和整合耳病学来卓越的传统 通过基于人群的研究，包括全面的耳诺测试和功能测量。