Southeast Resource Center for Integrated Metabolomics (SECIM)

东南综合代谢组学资源中心 (SECIM)

基本信息

批准号：
8732635
负责人：
ARTHUR S EDISON
金额：
$ 199.16万
依托单位：
UNIVERSITY OF FLORIDA
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-09-11 至 2018-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8732635
关键词：
Advisory Committees Animals Basic Science Bioinformatics Biological Markers Blood Banks Businesses Chemicals Chemistry Clinical Clinical Research Clinical Sciences Collaborations Complement Data Data Analyses Data Quality Development Educational workshop Feasibility Studies Florida Funding Future Galaxy Goals Grant Growth Ice Image Institutes Isotopes Jews Joints Laboratories Lead Life London Mass Spectrum Analysis Measurement Measures Medical Medical Research Metabolism Methodology Methods Modeling Monitor Mutation NMR Spectroscopy Nuclear Nuclear Magnetic Resonance Pilot Projects Preparation Principal Investigator Protocols documentation Recovery Reference Standards Research Institute Resources Running Sampling Scheme Science Scientist Services Structure Techniques Technology Testing Tissue Banking Tissue Banks Tissues Training Training and Education Translational Research Universities Work analytical tool base biobank college cost improved in vivo instrumentation magnetic field metabolomics operation outreach phenome research study response tool user-friendly web site

项目摘要

DESCRIPTION: The Southeast Resource Center for Integrated Metabolomics (SECIM) integrates existing strengths to create a comprehensive resource for basic and clinical scientists to obtain state-of-the-art metabolomics data and analyses. SECIM offers services in MS- and NMR-based metabolomics with partner Sanford-Burnham (Orlando, Fla.), who also contributes to Promotion & Outreach. We will offer a fully integrated platform for statistical analysis created by our Bioinformatics Core. Biomarkers will be identified by NMR and MS, with new methods for de novo structure prediction with the Bruschweiler lab at Florida State University and joint NMR/MS analysis with the Nicholson group at Imperial College. A service will be provided to SECIM users by the Prestegard lab at the University of Georgia for dynamic nuclear polarization (DNP) substrates that will be used by our partner, the AMRIS Facility, for monitoring of metabolism in living animals. SECIM users will also be able to conduct isotopic ratio outlier analysis (IROA) experiments to measure global metabolomic changes in response to external perturbations or mutations using LC/MS through our partnership with NextGen Metabolomics and Thermo Fisher. SECIM will provide ex vivo tissue analysis using MALDl-imaging (MS) and HRMAS (NMR) techniques, and users can also access an extensive tissue and blood bank through the UF Clinical and Translational Science Institute Biorepository. SECIM technical cores: 1) Mass Spectrometry Services for global and targeted metabolomics (Garrett and Gardell, Co-PIs) 2) NMR spectroscopy for global metabolomics and biomarker identification (Edison, PI) 3) Advanced MS for biomarker identification, imaging mass spectrometry, and IROA (Yost, PI) 4) Bioinformatics for SECIM pipeline development and analysis (Mclntyre, PI) The Promotion & Outreach Core (Conlon and Smith, Co-PIs) brings everything together by expanding the user base and providing education and training in SECIM capabilities. The Administrative Core coordinates all activities and will develop and implement a sustainable business model that will be self-supporting in years 6 and beyond.

描述：东南综合代谢组学资源中心 (SECIM) 整合现有优势，为基础和临床科学家创建综合资源，以获得最先进的代谢组学数据和分析。 SECIM 与合作伙伴 Sanford-Burnham（佛罗里达州奥兰多）一起提供基于 MS 和 NMR 的代谢组学服务，Sanford-Burnham 还为推广和外展做出了贡献。我们将提供一个完全集成的统计分析平台由我们的生物信息学核心。生物标志物将通过 NMR 和 MS 进行鉴定，并与佛罗里达州立大学的 Bruschweiler 实验室合作进行从头结构预测的新方法，并与帝国理工学院的 Nicholson 小组进行联合 NMR/MS 分析。佐治亚大学 Prestegard 实验室将向 SECIM 用户提供动态核极化 (DNP) 底物服务，我们的合作伙伴 AMRIS 设施将使用该服务来监测活体动物的新陈代谢。通过我们与 NextGen Metabolomics 和 Thermo Fisher 的合作，SECIM 用户还能够使用 LC/MS 进行同位素比异常值分析 (IROA) 实验，以测量响应外部扰动或突变的全球代谢组变化。 SECIM 将使用 MALD1 成像 (MS) 和 HRMAS (NMR) 技术提供离体组织分析，用户还可以通过佛罗里达大学临床和转化科学研究所生物存储库访问广泛的组织和血库。 SECIM 技术核心： 1) 用于全球和靶向代谢组学的质谱服务（Garrett 和 Gardell，Co-PI） 2) 用于全球代谢组学和生物标志物鉴定的 NMR 波谱（Edison，PI） 3) 用于生物标志物鉴定、成像质谱、和 IROA（Yost，PI）4）用于 SECIM 管道开发和分析的生物信息学（Mclntyre，PI） Outreach Core（Conlon 和 Smith，联合 PI）通过扩大用户群并提供 SECIM 功能的教育和培训将一切整合在一起。行政核心协调所有活动，并将开发和实施可持续的商业模式，该模式将在第 6 年及以后实现自给自足。