The Southeastern Center for Microscopy of Macromolecular Machines (SECM4)

东南高分子机器显微镜中心 (SECM4)

• 批准号: 10675030
• 负责人: SCOTT M STAGG
• 金额: $ 120.38万
• 依托单位: FLORIDA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2027-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10675030
• 关键词: 3-Dimensional; Address; Air; Buffers; Centrifugation; Charge; Client; Collaborations; Cryo-electron tomography; Cryoelectron Microscopy; Data; Data Analyses; Data Collection; Devices; Educational workshop; Explosion; Fees; Florida; Generations; Grant; Internet; Investments; Microscope; Microscopy; Monitor; Negative Staining; Persons; Preparation; Research Personnel; Resolution; Resources; Sampling; Scanning; Services; Site; Specimen; Structure; Time; Titan; Training; Travel; United States National Institutes of Health; Universities; Water; air sampling; computerized data processing; cost; high throughput screening; improved; instrumentation; interest; member; microscopic imaging; particle; preservation; reconstruction; sample fixation; screening; structural biology; theories; tomography

Abstract The Southeastern Center for Microscopy of MacroMolecular Machines (SECM4), at Florida State University (FSU) will be a service center that will enable sample preparation and cryogenic electron microscopy (cryo-EM) imaging of specimens for high-resolution biomolecular structure determination. The center will be led by Dr. Scott Stagg and Dr. Kenneth Taylor and will feature two staff members with complimentary expertise. This will be the second generation of the SECM4. The first generation provided high-resolution cryo-EM data collection for cryo-EM experts. In the new generation of the SECM4, we are expanding the scope of the resource by offsetting the cost of collecting high-resolution data on the microscope for all users with a very modest usage fee and also by offering a large number of new services including cryo-EM specimen optimization, specimen preparation, specimen screening, high-resolution data collection, routine single particle data analysis, and training for all aspects of cryo- EM from specimen preparation to processing. We will target users in the greater Southeast to be clients for the center, specifically focusing expanding into the underserved IDeA states that have not benefitted from the current explosion in cryo-EM due to the extraordinary costs that serve as an entry barrier. Our approach will enable exceptional economies of scale because we will: 1) enable users at universities across the Southeast to gain entry the cryo-EM field without making multi-million dollar investments in instrumentation, 2) by offering screening and specimen optimization services, we will address the biggest bottleneck in cryo-EM right now, which is preparation of cryo-EM samples that will reconstruct to high-resolution, 3) by offering in person training and offsetting the travel costs, we will enable interested users to gain entry into the field of cryo-EM without having to seek out a collaboration with an already saturated cryo-EM expert.

抽象的 佛罗里达州东南大分子机器显微镜中心 (SECM4) 大学（FSU）将成为一个服务中心，实现样品制备和低温电子 用于高分辨率生物分子结构测定的标本显微镜（冷冻电镜）成像。 该中心将由 Scott Stagg 博士和 Kenneth Taylor 博士领导，并有两名工作人员 具有免费的专业知识。这将是 SECM4 的第二代。第一代 为冷冻电镜专家提供高分辨率冷冻电镜数据采集。在新一代的 SECM4，我们正在通过抵消收集高分辨率的成本来扩大资源范围 显微镜上的数据为所有用户提供非常低廉的使用费，并且还提供大量 新服务包括冷冻电镜标本优化、标本制备、标本筛选、 高分辨率数据收集、常规单粒子数据分析以及冷冻各个方面的培训 EM 从样品制备到加工。我们将把大东南地区的用户作为目标客户 对于该中心来说，特别注重扩展到尚未受益的服务不足的 IDeA 州 由于高昂的成本成为进入壁垒，目前冷冻电镜的爆炸式增长。我们的 该方法将实现卓越的规模经济，因为我们将：1）为大学用户提供支持 跨越东南部进入冷冻电镜领域，而无需进行数百万美元的投资 仪器，2) 通过提供筛选和样本优化服务，我们将解决 目前冷冻电镜的最大瓶颈是准备用于重建的冷冻电镜样本 到高分辨率，3）通过提供亲自培训和抵消差旅费用，我们将能够 有兴趣的用户无需寻求与其他公司的合作即可进入冷冻电镜领域 冷冻电镜专家已经饱和。