Clinical Research and Assay Support

临床研究和检测支持

基本信息

批准号：
10697890
负责人：
Mary Walter
金额：
$ 125.15万
依托单位：
NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10697890
关键词：
Accounting Aliquot Bar Codes Biological Biological Assay Biological Markers Biological Specimen database Blood Calorimetry Clinical Clinical Research Collection Data Databases Diabetes Mellitus Endocrine Ensure Enteroglucagon Food Genomic DNA Hormones Hour Human Instruction International Label Leptin Liquid substance Measurement Measures Metabolic Methods National Institute of Diabetes and Digestive and Kidney Diseases Online Systems Oxidative Stress Plasma Preparation Principal Investigator Process Protocols documentation Reproducibility Research Research Personnel SARS-CoV-2 antibody Sampling Serology Services Ships Site Solid Specimen Statutes and Laws System Technology Testing Tube Urine Whole Blood adipokines adiponectin biobank cytokine des-n-octanoyl ghrelin genetic testing ghrelin glucagon-like peptide 1 preservation programs service utilization testing services

项目摘要

The Clinical Research Core is responsible for processing human samples from numerous on going clinical studies as well as studies that have been completed. Once the samples are acquired we process it according to the instructions on the protocol, aliquot, label and store the samples for the various principal investigators as per their instructions. We are currently processing an average of 1200 samples/week. All samples processed in the lab are set up with a tracking number and entered into a web based database which is maintained by the Core lab. Studies that have started since January 2008 are labeled and tracked using a 2D-barcode system. The Core lab currently stores 250K human biospecimen. The Core also ships and tracks human biospecimen for the PIs within the US as well as internationally. In addition to the biorepository services, the Clinical Research Core also provides assay support for analyses which are currently not available at DLM. We have tested and validated numerous methods to measure total and active Glucagon-like Peptide-1, Acyl Ghrelin, Desacyl Ghrelin, Leptin, Adiponectin, Oxyntomodulin and several other markers in human plasma. Recently we have added serology and neutralization assays against antibodies to SARS-CoV-2. The Clinical core also isolates genomic DNA for several studies where gDNA is required for gene testing. The lab also provides a testing service utilizing a Parr Bomb Calorimeter. The bomb calorimetry service provides a method for obtaining heats of combustion for liquid and solid samples. We are currently developing a method for calorimetric analysis of fecal, urine and food samples.

临床研究核心负责处理许多正在进行的临床研究以及已完成的研究中的人类样本。获取样品后，我们根据协议的说明，等分试样，标记和存储各种主要研究人员的样品，按照其说明进行处理。我们目前平均每周处理1200个样本。实验室中处理的所有样本均使用跟踪号进行设置，并输入基于Web的数据库，该数据库由Core Lab维护。自2008年1月以来开始的研究使用2D-Barcode系统标记和跟踪。核心实验室目前存储250k人类生物测量。该核心还在美国境内以及国际上的PIS发货并追踪人类的生物测量。除了生物验证服务外，临床研究核心还为DLM目前尚不可用的分析提供了测定支持。我们已经测试并验证了众多方法，用于测量人类血浆中总血浆中总和活跃的胰高血糖素样肽-1，酰基生长素蛋白，乙酰生长素蛋白，瘦素，脂联素，氧胞毒素和其他几种标记。最近，我们增加了针对SARS-COV-2抗体的血清学和中和测定。临床核还分离出基因测试需要GDNA的几项研究基因组DNA。该实验室还提供了利用Parr炸弹量量的测试服务。炸弹量热法提供了一种获取液体和固体样品燃烧热的方法。我们目前正在开发一种用于粪便，尿液和食物样品的量热分析的方法。