Virtual Reality Augmented Hands-on Cryo-EM Training

虚拟现实增强冷冻电镜实践培训

基本信息

批准号：
10633101
负责人：
Yingjie Chen
金额：
$ 12.25万
依托单位：
PURDUE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-05-01 至 2025-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10633101
关键词：
3-Dimensional Address Adopted Area Biological Biomedical Research Biophysics COVID-19 pandemic Categories Cell physiology Chemistry Clip Collaborations Color Communities Companions Complement Complex Computer Graphics Computer software Cryo-electron tomography Cryoelectron Microscopy Data Collection Development Plans Devices Disease Outbreaks Education Educational Curriculum Electron Microscope Electron Microscopy Electron Microscopy Facility Environment Equipment Evaluation Event Exercise Feedback Freezing GP2 gene General Population Gestures Goals Hand Health Human Impairment In Situ International Interview Knowledge Learning Macromolecular Complexes Methods Modeling Modernization Nobel Prize Online Systems Outcome Preparation Procedures Process Protocols documentation Research Research Institute Research Personnel Resolution Risk SARS-CoV-2 spike protein Safety Sampling Science Scientist Services Site Specimen Speed Structure Study Subject Supervision Surveys Sustainable Development System Technology Thinness Time Tissue Sample Training Training Activity Universities Vendor Virtual and Augmented reality Virus Virus Diseases Writing Zika Virus cost design experience eye hand coordination fluorescence microscope hazard human subject improved in vivo innovation insight instrument instrumentation learning materials lectures light microscopy multimodality operation outreach particle physical process receptor reconstruction simulation structural biology theories three dimensional structure tool virtual virtual reality web site

项目摘要

Project Summary Cryo-electron microscopy (cryoEM) revolutionized structural biology, providing a powerful method to study biological specimens in their native environment. However, a major bottleneck hindering the wider use of cryoEM is the lack of trained professionals capable of proficiently operating the instruments. CryoEM is complex and involves, in addition to an extensive biophysical theoretical background, operation of multiple devices that requires strict procedures, as well as accurate hand-eye coordination as mistakes in operation can damage expensive instrumentation. Current best-practices for user training involve repeated on-site hands-on practice and training with one-on-one supervision by experts. The high demand for instrument usage and staff assistance for cryoEM data collection often limits the opportunities for hands-on training needed by new cryoEM users. The goal of this project is to develop a virtual reality (VR) augmented interactive training system, CryoVR, to provide virtual hands-on, engaging, and self-paced cryoEM training to familiarize novice users with operational procedures, thereby reducing the need for one-on-one training and on-site practice on costly, in-demand cryoEM devices. Additionally, we see the benefit of this training platform as a review exercise for users who may have gone through long periods between instrument usage. We pursue this goal with three aims: Aim 1) Expanding the current CryoVR project to cover a wider range of instruments in single particle cryoEM, cryo-electron tomography, and cryo-correlative light and electron microscopy. This will address the need for orienting new users to cryoEM instruments, developing virtual mastery over a sequence of operations to perform specific key tasks from plunge-freezing of sample grids to TEM operation, and providing sufficient review for returning non- expert users. It is designed to be an integrated part of the cryoEM education curriculum as a pre-training before onsite hands-on training on the physical instruments; Aim 2) Improving users’ learning experience through new interactive features, such as guided, multimodal learning embedded in VR, enabling lab layout customization, personalized customization to enhance inclusion and accessibility, hazard simulation for safety training, and refining hand-gestures and reinforcing constraints for more faithful simulation of the physical actions; and Aim 3) Dissemination, evaluation, and sustainable development. We will evaluate CryoVR’s instructional materials, their impact, and outcomes through voluntary human subject studies and expert interviews. We will establish distribution channels and sustainable development plans to maximize the long-term impact of CryoVR on the community and public. The proposed approach is innovative in cryoEM training and will be effective, efficient, and engaging due to the applicability of modern VR technologies and education theories. This project is significant as it will greatly reduce the time, cost, and risk of cryoEM training, which in turn will lead to increased capacity for facilities to train new users. The low-cost VR hardware and easily accessible CryoVR software will benefit all cryoEM facilities and their users on a national and international scale.

项目概要冷冻电子显微镜 (cryoEM) 彻底改变了结构生物学，提供了强大的研究方法然而，阻碍冷冻电镜更广泛使用的一个主要瓶颈。缺乏能够熟练操作仪器的训练有素的专业人员是复杂且困难的。除了广泛的生物物理理论背景外，还涉及多种设备的操作需要严格的程序，以及准确的手眼协调，操作错误可能会损坏当前昂贵的仪器当前的用户培训最佳实践包括重复的现场实践练习。专家一对一监督的培训对仪器使用和工作人员协助的要求很高。 CryoEM 数据收集的局限性通常会限制新的 CryoEM 用户所需的实践培训机会。该项目的目标是开发虚拟现实（VR）增强交互式培训系统CryoVR，以提供虚拟实践、引人入胜且自定进度的冷冻电镜培训，让新手用户熟悉操作程序，从而减少对昂贵的、按需冷冻电镜的一对一培训和现场实践的需要此外，我们还认为该培训平台作为对可能拥有设备的用户进行审查练习的好处。我们通过仪器使用经历了很长一段时间来实现这一目标：目标 1) 扩展。当前的 CryoVR 项目涵盖单粒子冷冻电镜、冷冻电子领域更广泛的仪器断层扫描、冷冻相关光学和电子显微镜这将满足新方向的需求。用户可以使用冷冻电镜仪器，虚拟掌握一系列操作以执行特定的关键操作从样品网格的骤冷到 TEM 操作，并为返回的非样品提供充分的审查它被设计为冷冻电镜教育课程的一个组成部分，作为之前的预培训。物理仪器现场实践培训；目标2）通过新的方式改善用户的学习体验；交互功能，例如嵌入 VR 的引导式多模式学习，可实现实验室布局定制，个性化定制以增强包容性和可及性，安全培训的危险模拟，以及改进手势并加强约束，以更忠实地模拟身体动作和目标 3) 我们将评估 CryoVR 的教学材料及其可持续发展。我们将通过自愿人类受试者研究和专家访谈来确定影响和结果。分销渠道和可持续发展计划，以最大限度地发挥 CryoVR 对市场的长期影响所提出的方法在冷冻电镜培训方面具有创新性，并且将是有效、高效、由于现代 VR 技术和教育理论的适用性，该项目具有吸引力。意义重大，因为它将大大减少冷冻电镜培训的时间、成本和风险，进而导致增加低成本 VR 硬件和易于使用的 CryoVR 软件将提高设施培训新用户的能力。使国内和国际范围内的所有冷冻电镜设施及其用户受益。