Virtual Reality Augmented Hands-on Cryo-EM Training

虚拟现实增强冷冻电镜实践培训

• 批准号: 10223529
• 负责人: Yingjie Chen
• 金额: $ 12.25万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-05-01 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10223529
• 关键词: 3-Dimensional; Address; Adopted; Area; Biological; Biomedical Research; Biophysics; COVID-19 pandemic; Categories; Cell physiology; Chemistry; Color; Communities; Companions; Complement; Complex; Computer Graphics; Computer software; Cryo-electron tomography; Cryoelectron Microscopy; Custom; Data Collection; Development Plans; Devices; Disease Outbreaks; Education; Educational Curriculum; Electron Microscope; Electron Microscopy; Electron Microscopy Facility; Environment; Equipment; Evaluation; Event; Exercise; Feedback; Freezing; GP2 gene; General Population; Gestures; Goals; Hand; Health; Human; Immersion; Impairment; In Situ; International; Interview; Knowledge; Learning; Macromolecular Complexes; Methods; Modeling; Modernization; Nobel Prize; Online Systems; Outcome; Preparation; Procedures; Process; Protocols documentation; Research; Research Institute; Research Personnel; Resolution; Risk; SARS-CoV-2 spike protein; Safety; Sampling; Science; Scientist; Services; Site; Specimen; Speed; Structure; Study Subject; Supervision; Surveys; Sustainable Development; System; Technology; Thinness; Time; Tissue Sample; Training; Training Activity; Universities; Vendor; Virtual and Augmented reality; Virus; Virus Diseases; Zika Virus; base; cost; design; experience; eye hand coordination; fluorescence microscope; hazard; human subject; improved; in vivo; innovation; insight; instrument; instrumentation; laboratory equipment; learning materials; lectures; light microscopy; multimodality; operation; outreach; particle; physical process; receptor; reconstruction; simulation; structural biology; theories; three dimensional structure; tool; virtual; virtual reality; web site

Project Summary Cryo-electron microscopy (cryoEM) revolutionized structural biology, providing a powerful method to study biological specimens in their native environment. However, a major bottleneck hindering the wider use of cryoEM is the lack of trained professionals capable of proficiently operating the instruments. CryoEM is complex and involves, in addition to an extensive biophysical theoretical background, operation of multiple devices that requires strict procedures, as well as accurate hand-eye coordination as mistakes in operation can damage expensive instrumentation. Current best-practices for user training involve repeated on-site hands-on practice and training with one-on-one supervision by experts. The high demand for instrument usage and staff assistance for cryoEM data collection often limits the opportunities for hands-on training needed by new cryoEM users. The goal of this project is to develop a virtual reality (VR) augmented interactive training system, CryoVR, to provide virtual hands-on, engaging, and self-paced cryoEM training to familiarize novice users with operational procedures, thereby reducing the need for one-on-one training and on-site practice on costly, in-demand cryoEM devices. Additionally, we see the benefit of this training platform as a review exercise for users who may have gone through long periods between instrument usage. We pursue this goal with three aims: Aim 1) Expanding the current CryoVR project to cover a wider range of instruments in single particle cryoEM, cryo-electron tomography, and cryo-correlative light and electron microscopy. This will address the need for orienting new users to cryoEM instruments, developing virtual mastery over a sequence of operations to perform specific key tasks from plunge-freezing of sample grids to TEM operation, and providing sufficient review for returning non- expert users. It is designed to be an integrated part of the cryoEM education curriculum as a pre-training before onsite hands-on training on the physical instruments; Aim 2) Improving users’ learning experience through new interactive features, such as guided, multimodal learning embedded in VR, enabling lab layout customization, personalized customization to enhance inclusion and accessibility, hazard simulation for safety training, and refining hand-gestures and reinforcing constraints for more faithful simulation of the physical actions; and Aim 3) Dissemination, evaluation, and sustainable development. We will evaluate CryoVR’s instructional materials, their impact, and outcomes through voluntary human subject studies and expert interviews. We will establish distribution channels and sustainable development plans to maximize the long-term impact of CryoVR on the community and public. The proposed approach is innovative in cryoEM training and will be effective, efficient, and engaging due to the applicability of modern VR technologies and education theories. This project is significant as it will greatly reduce the time, cost, and risk of cryoEM training, which in turn will lead to increased capacity for facilities to train new users. The low-cost VR hardware and easily accessible CryoVR software will benefit all cryoEM facilities and their users on a national and international scale.

项目摘要 冷冻电子显微镜（Cryoem）彻底改变了结构生物学，提供了一种强大的研究方法 生物标本在其本地环境中。但是，一种主要的瓶颈阻碍了更广泛的冷冻使用 是缺乏能够正确操作工具的训练有素的专业人员。冷冻物很复杂， 除了广泛的生物物理理论背景外，还涉及多个设备的操作 需要严格的程序以及准确的手眼协调，因为操作中的错误可能会损害 昂贵的仪器。当前用于用户培训的最佳实践涉及重复现场动手实践 并通过专家一对一的监督进行培训。对仪器使用和员工协助的高需求 对于冷冻数据，收集通常会限制新冷冻用户所需的动手培训的机会。 该项目的目标是开发虚拟现实（VR）增强互动培训系统Cryovr，以提供 虚拟动手，引人入胜和自节奏的冷冻培训，以使新颖的用户使用运营 程序，从而减少了一对一培训和现场练习的需求 设备。此外，我们认为该培训平台的好处是对可能拥有的用户的审查练习 在仪器使用之间经历了长时间的经历。我们以三个目标追求这个目标：目标1）扩展 当前的Cryovr项目涵盖单个粒子冷冻，冷冻电子的更广泛的仪器 断层扫描以及冷冻的光和电子显微镜。这将满足定向新的需求 用户进行冷冻仪器，通过一系列操作进行虚拟掌握以执行特定的密钥 从样品网格冻结到TEM操作的任务，并提供足够的审查以返回非 - 专家用户。它旨在成为冷冻教育课程的一部分，作为预培训 现场动手训练物理仪器；目标2）通过新的 交互式功能，例如在VR中嵌入的有指导性的多模式学习，启用实验室布局自定义， 个性化定制以增强包含和可访问性，安全培训的危害模拟以及 提炼手及以来，并加强对身体行为的忠实模拟的约束；和目标3） 传播，评估和可持续发展。我们将评估Cryovr的教学材料，他们 通过自愿人类学科研究和专家访谈的影响以及结果。我们将建立 分销渠道和可持续发展计划，以最大化Cryovr对 社区和公众。拟议的方法在冷冻培训中具有创新性，将有效，有效， 并由于现代VR技术和教育理论的适用性而参与。这个项目是 意义重大，因为它将大大降低冷冻训练的时间，成本和风险，从而导致增加 设施培训新用户的能力。低成本VR硬件和易于访问的Cryovr软件将 在国家和国际规模上使所有冷冻设施及其用户受益。