Abberior Infinity Line Upright 3D STED/Confocal Microscope

Abberior Infinity Line 正置 3D STED/共焦显微镜

• 批准号: 10632948
• 负责人: Anthony J Ricci
• 金额: $ 145.47万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-04-15 至 2024-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10632948
• 关键词: 3-Dimensional; Aging; Basic Science; Biological; Clinical; Collaborations; Core Facility; Data Analyses; Detection; Development; Devices; Diagnostic; Equipment; Experimental Designs; Fostering; Health; Image; Lasers; Light; Lighting; Maintenance; Malignant Neoplasms; Microscope; Mission; Noise; Optics; Otolaryngology; Pharmaceutical Preparations; Positioning Attribute; Postdoctoral Fellow; Research; Research Personnel; Resolution; Resources; Rotation; Running; Schedule; Scientist; Services; Signal Transduction; System; Technical Expertise; Technology; Training; United States National Institutes of Health; Work; adaptive optics; detector; experience; fluorophore; imaging system; improved; live cell imaging; nervous system disorder; next generation; sensory integration; student participation; superresolution imaging; technology development; ultra high resolution

Abstract: The Department of Otolaryngology has active research across all of its divisions. The department has run a core facility for over 12 years that is accessible to more than 100 researchers within our department. The core facility is a training facility that fosters collaboration across technical expertise and biological questions. We are requesting the purchase of a super- resolution imaging system that will be placed into this core facility where training, scheduling and maintenance can be managed by experienced staff. The Abberior STED system incorporates the most up-to-date technology to provide the most versatile super- resolution system available. The use of adaptive optics and adaptive illumination allows for high-resolution live cell imaging. The incorporation of avalanche diodes provides the most sensitive detectors available. Quad scanners maintain beam positioning and alignment while providing image rotation. Matrix array spectral detection offers the next generation of improved signal to noise by removing out focus light. STED FLIM allows for lower power laser to improve live-cell imaging. It further provides optical colocalization by using a single light path for multiple emissions. Multiple emissions are possible by separating fluorophores by lifetime rather than wavelength. There are no existing systems at Stanford so this new device will be critical for many scientists both within and outside of our department. Work within our department spans from basic science to translational to clinical. It ranges from cancer to aging, to neurological disorders, sensory integration and drug and diagnostic development. It is directly in line with the mission of NIH in terms of a primary focus on health-related research and training of the next-generation of scientists. Our core facility also serves as a training facility where we engage students, postdocs, residents, etc. and train them on the first principles of the technology development, experimental design and data analysis. Finally, the Department of Otolaryngology supports the core facility and will support this new addition by providing the space and the resources needed for staff as well as maintenance, service and oversight of the equipment.

抽象的： 耳鼻喉科部门在其所有部门进行了积极的研究。这 部门运营了一个超过12年的核心设施，可容纳100多个 我们部门的研究人员。核心设施是培养机构的培训设施 跨技术专长和生物学问题的协作。我们要求 购买将放入此核心设施的超分辨率成像系统 经验丰富的员工可以管理培训，日程安排和维护的地方。这 Abberior Sted系统结合了最新技术，以提供最新的技术 多功能超级分辨率系统可用。适应性光学和自适应的使用 照明允许进行高分辨率的活细胞成像。雪崩的合并 二极管提供了最敏感的探测器。四边形扫描仪保持光束 在提供图像旋转时定位和对齐。矩阵阵列光谱检测 通过删除聚焦光线来提供下一代改进的信号到噪声。 st FLIM允许低功率激光器改善活电池成像。它进一步提供光学 通过使用单个光路路径进行多个排放。多次排放是 通过寿命而不是波长将荧光团分开。没有 斯坦福大学的现有系统，因此该新设备对许多科学家都至关重要 我们部门内外。我们部门内的工作跨越基本 科学转化为临床。它的范围从癌症到衰老到神经系统 疾病，感觉整合，药物以及诊断发展。它直接排队 在NIH的任务中，主要关注与健康有关的研究和培训 科学家的下一代。我们的核心设施也是培训设施 我们与学生，博士后，居民等接触，并培训他们的第一原则 技术开发，实验设计和数据分析。最后，部门 耳鼻喉科支持核心设施，并将通过提供来支持这一新成员 员工所需的空间和资源以及维护，服务和 对设备的监督。