Whole slide scanner for translational neuroscience research

用于转化神经科学研究的全玻片扫描仪

• 批准号: 10629586
• 负责人: MICHAEL WONG
• 金额: $ 43.02万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-05-01 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10629586
• 关键词: Advisory Committees; Alzheimer' s Disease; Animal Model; Applications Grants; Brain; Brain Neoplasms; Charge; Clinical; Contract Services; Core Facility; Data; Data Analyses; Data Storage and Retrieval; Discipline; Disease; Epilepsy; Equipment; Faculty; Financial Support; Functional disorder; Funding; Funding Agency; Glass; Grant; Histologic; Hour; Human Resources; Image; Information Technology; Laboratories; Minor; Molecular Analysis; Motor Neuron Disease; Multiple Sclerosis; Neurologic; Neurology; Neurosciences; Neurosciences Research; Pain; Pathology; Principal Investigator; Research; Research Personnel; Resources; Sampling; Scanning; Services; Slide; Specimen; Spinal cord injury; Stroke; System; Time; Tissues; Training; United States National Institutes of Health; Universities; Washington; data management; digital pathology; experience; imaging system; instrumentation; medical schools; neonatal hypoxic-ischemic brain injury; nervous system disorder; neurogenetics; neuroimaging; new therapeutic target; operation; square foot; translational neuroscience; whole slide imaging

PROJECT SUMMARY/ABSTRACT Digital pathology has revolutionized clinical and research applications in the field of pathology and related disciplines. In particular, the advent of whole slide imaging (WSI) has allowed rapid acquisition, high-level comprehensive unbiased analysis, and convenient storage and transfer of histological data from entire glass slides of tissue specimens. WSI is especially conducive to neuroscience research, promoting conventional histological and advanced immunohistochemical and molecular analysis of brain samples. Since 2010, a heavily- utilized WSI system in the Alafi Neuroimaging Laboratory core facility at Washington University School of Medicine (WUSM) has helped advance translational neuroscience research in a variety of neurological and non- neurological disorders. With increasing demands for Alafi WSI services among NIH-funded users exceeding our current capacity and in conjunction with the planned opening of a new 600,000 square foot WUSM Neuroscience building with over 125 neuroscience laboratories and approximately 1000 neuroscientists, this shared instrumentation grant application proposes to acquire a new WSI system that will further facilitate and expand cutting-edge translational neuroscience at WUSM. The proposed WSI system (Epredia Pannoramic 250 Flash III) will be utilized by ten Major Users and eleven Minor Users performing translational neuroscience research in animal models of multiple neurological disorders, including Alzheimer's disease, stroke, epilepsy, neurogenetic disorders, neonatal hypoxia-ischemia, motor neuron disease, multiple sclerosis, spinal cord injury, pain, and brain tumors. Funded by over 30 NIH grants, Major and Minor Users will account for at least 75% of accessible user time, with the remaining time available for new users and other researchers within the Neuroscience building and elsewhere on campus. As the WSI system can perform automated, unattended scanning acquisition for some studies, we anticipate operation for close to 100 hours per week. The proposed system will be housed in the Alafi Neuroimaging Laboratory, relocating to the new neuroscience building in 2023, and be managed by the Hope Center for Neurological Disorders, an interdepartmental research center administered within the Department of Neurology. The Hope Center will commit financial support and resources for renovation of space and ongoing facility expenses, such as core personnel and service contracts, through an existing recharge center and other funding sources. The principal investigator and experienced core support staff will assist and train users in equipment use and data analysis. Data storage and management will be supported by a WUSM Information Technology services group. An Advisory Committee, consisting of Major Users and other faculty with administrative or scientific expertise related to imaging, will oversee the equipment planning and utilization. Overall, a new WSI will greatly promote the next wave of advances in understanding the pathophysiology of the most significant neurological diseases and developing novel targeted therapies for these devastating disorders.

项目摘要/摘要 数字病理已经彻底改变了病理和相关领域的临床和研究应用 学科。特别是，整个幻灯片成像的出现（WSI）允许快速获取，高级 全面的无偏分析，以及从整个玻璃中的组织学数据方便的存储和传递 组织标本的载玻片。 WSI特别有利于神经科学研究，促进常规 脑样品的组织学和晚期免疫组织化学和分子分析。自2010年以来， 华盛顿大学学校的ALAFI神经影像学实验室核心设施中利用WSI系统 医学（WUSM）有助于推进各种神经和非神经科学的转化神经科学研究 神经系统疾病。 NIH资助的用户中对Alafi WSI服务的需求不断提高，超出了我们 当前容量以及计划开放新的600,000平方英尺的WUSM神经科学 该建筑有超过125个神经科学实验室和大约1000个神经科学家的建筑 仪器赠款申请提议获得新的WSI系统，以进一步促进和扩展 WUSM的尖端翻译神经科学。拟议的WSI系统（Epredia Pannoramic 250 Flash iii）将由十位主要用户和11个未成年人使用，从事转化神经科学研究 多种神经系统疾病的动物模型，包括阿尔茨海默氏病，中风，癫痫，神经遗传学 疾病，新生儿缺氧 - 缺血性，运动神经元疾病，多发性硬化症，脊髓损伤，疼痛和 脑肿瘤。由30多个NIH赠款资助，主要用户和未成年人至少占可访问的75％ 用户时间，剩余时间可用于神经科学大楼内的新用户和其他研究人员 以及校园的其他地方。由于WSI系统可以执行自动，无人看管的扫描获取 一些研究，我们预计每周运行近100个小时。 拟议的系统将安置在Alafi神经影像学实验室中，迁移到新的神经科学 于2023年建造，并由希望神经系统疾病中心进行管理，这是一项跨部门研究 中心在神经病学系内。希望中心将提供财政支持，并 用于翻新空间和正在进行的设施费用的资源，例如核心人员和服务合同， 通过现有的充电中心和其他资金来源。首席调查员和经验丰富的核心 支持人员将协助和培训用户进行设备使用和数据分析。数据存储和管理将 由WUSM信息技术服务小组的支持。咨询委员会，由主要 用户和其他具有与成像有关的行政或科学专业知识的教师将监督设备 计划和利用。总体而言，新的WSI将大大促进下一步的了解 最重要的神经系统疾病的病理生理学，并为这些疾病开发新的靶向疗法 毁灭性疾病。