Leica Stellaris 8 Confocal for Imaging Core

Leica Stellaris 8 共焦成像核心

• 批准号: 10630453
• 负责人: Amy Beaven
• 金额: $ 60万
• 依托单位: UNIV OF MARYLAND, COLLEGE PARK
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10630453
• 关键词: 13 year old; 14 year old; Biological Sciences; Biology; Cell Communication; Cells; Cellular biology; Contract Services; Dedications; Developmental Biology; Ensure; Faculty; Funding; Future; Image; Immunology; Knowledge; Lasers; Light; Maryland; Microscope; Minor; Molecular; Molecular Biology; Neurobiology; Physiologic pulse; Preparation; RNA; Regulation; Research; Research Personnel; Research Project Grants; Research Support; Sampling; Scanning; Services; Students; Synapses; System; Technology; Training; United States National Institutes of Health; Universities; Visual; base; college; confocal imaging; design; detector; equipment acquisition; experience; extracellular; fluorescence lifetime imaging; imaging capabilities; imaging software; imaging system; instrument; neuroregulation; next generation; pathogen; protein transport

The University of Maryland College Park (UMD) requests $600,000 to purchase a critically-needed Leica Stellaris 8 FALCON (FAst Lifetime CONtrast) laser scanning confocal microscope. A diverse base of 14 Life Science researchers (12 of whom are NIH-funded), who are all making significant and pioneering contributions to cross- disciplinary research at the interface between cell biology, molecular biology neurobiology, developmental biology, immunology, and host-pathogen interactions, will immediately benefit from the transformative imaging capabilities of the instrument. Need for the instrument is great, as it will replace a well-used 14-year-old Leica SP5x confocal that can no longer be upgraded and will lose guaranteed service support at the end of 2022. The IC currently houses two other confocal systems, a 13-year-old Zeiss LSM 710 and a Zeiss LSM 980 Airyscan 2, but the existing systems at UMD cannot accommodate all the imaging needs of the current SP5x users plus the anticipated future needs of the major and minor users on this proposal, many of whom are starting new imaging projects. The requested instrument is specifically designed to maintain the capabilities of the existing SP5x, plus provide new cutting-edge technologies needed to enhance the users’ NIH-funded research beyond what is currently possible via any other imaging system on campus. The Stellaris 8 will be equipped with a next- generation pulsed white light laser, multiple high-efficiency Power HyD detectors, and integrated fluorescence lifetime imaging (FLIM) software and support. The Stellaris 8 will be housed and maintained in the well- established Imaging Core (IC), which is managed by an experienced facility director and is accessible to all UMD faculty, students, and their collaborators. The IC makes significant contributions to NIH-supported research at UMD by providing extensive imaging services ranging from advice on sample preparation to comprehensive instrument training and assistance with image acquisition. The requested scanning confocal microscope will advance current knowledge on a broad range of research projects, including host-pathogen interactions, the molecular composition of synaptic connections in mammalian visual circuits, regulation of neuromodulatory transmitters, protein trafficking, cell-cell communication, and the biology of extracellular RNAs. UMD has dedicated significant matching funds toward the purchase of the instrument and operational support. Furthermore, support at the departmental level will ensure that the instrument is covered under a service contract for the lifetime of the instrument.

马里兰大学帕克分校 (UMD) 请求 60 万美元购买急需的 Leica Stellaris 8 FALCON（快速寿命对比）激光扫描共焦显微镜 14 生命科学的多样化基础。 研究人员（其中 12 名由 NIH 资助），他们都在跨学科领域做出了重大和开创性的贡献 细胞生物学、分子生物学、神经生物学、发育学之间的交叉学科研究 生物学、免疫学和宿主-病原体相互作用，将立即受益于变革性成像 对该仪器的需求很大，因为它将取代已经使用了 14 年的徕卡。 SP5x 共焦无法再升级，并将在 2022 年底失去有保证的服务支持。 IC 目前拥有另外两个共焦系统，即使用了 13 年的 Zeiss LSM 710 和 Zeiss LSM 980 Airyscan 2， 但 UMD 的现有系统无法满足当前 SP5x 用户的所有成像需求以及 主要和次要用户对该提案的预期未来需求，其中许多人正在开始新的成像 项目所要求的仪器是专门为保持现有 SP5x 的功能而设计的。 提供增强用户 NIH 资助的研究所需的新的尖端技术 目前，Stellaris 8 将配备一个下一代成像系统。 生成脉冲白光激光器、多个高效 Power HyD 探测器和集成荧光 Stellaris 8 将在井内安装和维护。 建立了成像核心 (IC)，由经验丰富的设施总监管理，所有 UMD 均可使用 IC 为 NIH 支持的研究做出了重大贡献。 UMD 提供广泛的成像服务，从样品制备建议到全面的成像服务 仪器培训和图像采集协助。 目前关于广泛研究项目的知识，包括宿主与病原体的相互作用、 哺乳动物视觉回路突触连接的分子组成，神经调节的调节 递质、蛋白质运输、细胞通讯和细胞外 RNA 生物学。 投入大量配套资金用于购买仪器和运营支持。 此外，部门一级的支持将确保该仪器包含在服务合同内 在仪器的使用寿命内。