Next-Generation Diagnostics for Acute Encephalitis
急性脑炎的下一代诊断
基本信息
- 批准号:10077591
- 负责人:
- 金额:$ 19.97万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2016
- 资助国家:美国
- 起止时间:2016-12-01 至 2021-11-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
PROJECT SUMMARY/ABSTRACT
Encephalitis is a heterogeneous syndrome defined by inflammation of the brain parenchyma leading to altered
mental status and frequently, many other neurologic signs and symptoms including seizures, paralysis,
hypoventilation and in some cases, death. Well over 100 viruses, autoantibodies, parasites, bacteria and fungi
cause encephalitis, but in at least half of cases, no etiology is identified. Failure to identify an etiologic agent
delays targeted therapies to patients and leads to empiric treatment trials that are sometimes ineffective and
other times, actually exacerbate the patient's underlying condition.
My goal in seeking a K08 Mentored Clinical Scientist Research Career Development Award is to acquire
the necessary training, practical experience and knowledge to make major advances in our understanding of
the causes of encephalitis. My long-term goal is to reduce the large number of idiopathic encephalitis cases by
enhancing a novel diagnostic platform. I hypothesize that a novel molecular approach will improve the limits of
detection for microbes in our next-generation sequencing (NGS) assay that aims to detect any class of microbe
in cerebrospinal fluid and brain tissue (Subaim 1A). I hypothesize that a novel statistical approach will help to
rigorously discriminate between microbial contamination vs. true infection in the metagenomic data collected
from our NGS assay (Subaim 1B). I will harness these new molecular and analytic approaches to more
frequently and accurately identify pathogens via metagenomic NGS in an ongoing, multicenter, prospective
cohort study of patients with idiopathic encephalitis (Aim 2).
During my self-designed fellowship in Neuro-Infectious Diseases at Massachusetts General Hospital and
Boston University's National Emerging Infectious Diseases Laboratories, I acquired a unique skillset and was
recruited back to UCSF as an Assistant Adjunct Professor in the Department of Neurology. I have put together
a world-class team of mentors and advisors to guide me through this career development phase in order to
launch myself successfully as an independent investigator. The research environment at UCSF is exceptional
and includes cutting edge scientists and state-of-the-art equipment in the California Institute of Quantitative
Biosciences (QB3) and the Sandler Neurosciences Building. My training plan is specifically designed to provide
advanced training in clinical research methods, statistics, bioinformatics and microbiology that will be
necessary to actualize these goals and position me to obtain R01 or equivalent funding as an independent
investigator. I also intend to be an active participant in the K-Scholars program.
项目摘要/摘要
脑炎是一种由脑实质的炎症定义的异质综合征,导致改变
精神状态,经常,许多其他神经系统症状和症状,包括癫痫发作,瘫痪,
不足驱动,在某些情况下是死亡。超过100多个病毒,自身抗体,寄生虫,细菌和真菌
引起脑炎,但在至少一半的情况下,未发现病因。无法识别病因
针对患者的延迟疗法的延迟,并导致有时无效的经验治疗试验
其他时候,实际上会加剧患者的潜在状况。
我寻求K08指导的临床科学家研究职业发展奖的目标是获得
我们对理解的重大进步的必要培训,实践经验和知识
脑炎的原因。我的长期目标是通过减少大量特发性脑炎病例
增强新颖的诊断平台。我假设一种新颖的分子方法将改善
我们的下一代测序(NGS)测定中的微生物检测,该测定旨在检测任何类别的微生物
在脑脊液和脑组织中(Subaim 1a)。我假设一种新颖的统计方法将有助于
严格区分微生物污染与收集的宏基因组数据中的真实感染
来自我们的NGS分析(Subaim 1b)。我将利用这些新的分子和分析方法
在正在进行的多中心中,经常,准确地通过元基因组NG识别病原体
对特发性脑炎患者的队列研究(AIM 2)。
在我在马萨诸塞州综合医院进行神经感染疾病的自我设计奖学金期间
波士顿大学的国家新兴传染病实验室,我获得了独特的技能,是
担任神经病学系的助理兼职教授,招募回到UCSF。我已经把
一个世界一流的导师和顾问团队,指导我进入这个职业发展阶段
作为独立调查员成功启动自己。 UCSF的研究环境非常出色
并包括加利福尼亚定量研究所的尖端科学家和最先进的设备
生物科学(QB3)和桑德勒神经科学大楼。我的培训计划是专门设计的
临床研究方法,统计,生物信息学和微生物学的高级培训将是
实现这些目标并定位我以获得R01或同等资金作为独立的必要
研究者。我还打算成为K-Scholars计划的积极参与者。
项目成果
期刊论文数量(30)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Meningitis Caused by the Live Varicella Vaccine Virus: Metagenomic Next Generation Sequencing, Immunology Exome Sequencing and Cytokine Multiplex Profiling.
- DOI:10.3390/v13112286
- 发表时间:2021-11-16
- 期刊:
- 影响因子:0
- 作者:Ramachandran PS;Wilson MR;Catho G;Blanchard-Rohner G;Schiess N;Cohrs RJ;Boutolleau D;Burrel S;Yoshikawa T;Wapniarski A;Heusel EH;Carpenter JE;Jackson W;Ford BA;Grose C
- 通讯作者:Grose C
Acute West Nile Virus Meningoencephalitis Diagnosed Via Metagenomic Deep Sequencing of Cerebrospinal Fluid in a Renal Transplant Patient.
- DOI:10.1111/ajt.14058
- 发表时间:2017-03
- 期刊:
- 影响因子:0
- 作者:Wilson MR;Zimmermann LL;Crawford ED;Sample HA;Soni PR;Baker AN;Khan LM;DeRisi JL
- 通讯作者:DeRisi JL
Hepatitis E Virus-Associated Meningoencephalitis in a Lung Transplant Recipient Diagnosed by Clinical Metagenomic Sequencing.
通过临床宏基因组测序诊断肺移植受者的戊型肝炎病毒相关脑膜脑炎。
- DOI:10.1093/ofid/ofx121
- 发表时间:2017
- 期刊:
- 影响因子:4.2
- 作者:Murkey JA;Chew KW;Carlson M;Shannon CL;Sirohi D;Sample HA;Wilson MR;Vespa P;Humphries RM;Miller S;Klausner JD;Chiu CY
- 通讯作者:Chiu CY
A novel cause of chronic viral meningoencephalitis: Cache Valley virus.
- DOI:10.1002/ana.24982
- 发表时间:2017-07
- 期刊:
- 影响因子:11.2
- 作者:Wilson MR;Suan D;Duggins A;Schubert RD;Khan LM;Sample HA;Zorn KC;Rodrigues Hoffman A;Blick A;Shingde M;DeRisi JL
- 通讯作者:DeRisi JL
Detection of Cryptococcus DNA by Metagenomic Next-generation Sequencing in Symptomatic Cryptococcal Antigenemia.
通过宏基因组下一代测序检测有症状的隐球菌抗原血症中的隐球菌 DNA。
- DOI:10.1093/cid/ciy1024
- 发表时间:2019
- 期刊:
- 影响因子:0
- 作者:Ramachandran,PrashanthS;Cresswell,FionaV;Meya,DavidB;Langelier,Charles;Crawford,EmilyD;DeRisi,JosephL;Boulware,DavidR;Wilson,MichaelR
- 通讯作者:Wilson,MichaelR
共 15 条
- 1
- 2
- 3
Michael R Wilson其他文献
Associations between litter size and specific herd level management factors in Ontario swine
安大略省猪产仔数与特定猪群水平管理因素之间的关联
- DOI:10.1016/0167-5877(94)00393-w10.1016/0167-5877(94)00393-w
- 发表时间:19951995
- 期刊:
- 影响因子:0
- 作者:C. Dewey;S. Martin;R. Friendship;B. Kennedy;Michael R WilsonC. Dewey;S. Martin;R. Friendship;B. Kennedy;Michael R Wilson
- 通讯作者:Michael R WilsonMichael R Wilson
assay on cerebrospinal fluid for the detection of tuberculous meningitis in HIV-infected adults
脑脊液检测用于检测成人 HIV 感染者的结核性脑膜炎
- DOI:
- 发表时间:20202020
- 期刊:
- 影响因子:0
- 作者:C. Quinn;E. Kagimu;Michael Okirworth;A. Bangdiwala;Gerald Mugumya;P. Ramachandran;Michael R Wilson;D. Meya;F. Cresswell;N. Bahr;D. BoulwareC. Quinn;E. Kagimu;Michael Okirworth;A. Bangdiwala;Gerald Mugumya;P. Ramachandran;Michael R Wilson;D. Meya;F. Cresswell;N. Bahr;D. Boulware
- 通讯作者:D. BoulwareD. Boulware
Volutraumaとatelectraumaでは炎症の度合いが異なる:肺血管内単球の人工呼吸器関連肺損傷における役割
容量伤和肺不张伤有不同程度的炎症:肺血管内单核细胞在呼吸机相关性肺损伤中的作用
- DOI:
- 发表时间:20152015
- 期刊:
- 影响因子:0
- 作者:若林健二;Michael R Wilson;Kate C Tatham;Kieran P O'Dea;高田正雄若林健二;Michael R Wilson;Kate C Tatham;Kieran P O'Dea;高田正雄
- 通讯作者:高田正雄高田正雄
共 3 条
- 1
Michael R Wilson的其他基金
Redefining Tuberculosis Meningitis with Metagenomics and Host Transciptomics
利用宏基因组学和宿主转录组学重新定义结核性脑膜炎
- 批准号:1035902910359029
- 财政年份:2019
- 资助金额:$ 19.97万$ 19.97万
- 项目类别:
Redefining Tuberculosis Meningitis with Metagenomics and Host Transciptomics
利用宏基因组学和宿主转录组学重新定义结核性脑膜炎
- 批准号:98909999890999
- 财政年份:2019
- 资助金额:$ 19.97万$ 19.97万
- 项目类别:
Redefining Tuberculosis Meningitis with Metagenomics and Host Transciptomics
利用宏基因组学和宿主转录组学重新定义结核性脑膜炎
- 批准号:1057684110576841
- 财政年份:2019
- 资助金额:$ 19.97万$ 19.97万
- 项目类别:
相似国自然基金
阿魏酸基天然抗氧化抗炎纳米药物用于急性肾损伤诊疗一体化研究
- 批准号:82302281
- 批准年份:2023
- 资助金额:30 万元
- 项目类别:青年科学基金项目
SGO2/MAD2互作调控肝祖细胞的细胞周期再进入影响急性肝衰竭肝再生的机制研究
- 批准号:82300697
- 批准年份:2023
- 资助金额:30 万元
- 项目类别:青年科学基金项目
基于hemin-MOFs的急性心肌梗塞标志物负背景光电化学-比色双模分析
- 批准号:22304039
- 批准年份:2023
- 资助金额:30 万元
- 项目类别:青年科学基金项目
RNA甲基转移酶NSUN2介导SCD1 mRNA m5C修饰调控急性髓系白血病细胞铁死亡的机制研究
- 批准号:82300173
- 批准年份:2023
- 资助金额:30 万元
- 项目类别:青年科学基金项目
基于IRF5/MYD88信号通路调控巨噬细胞M1极化探讨针刀刺营治疗急性扁桃体炎的机制研究
- 批准号:82360957
- 批准年份:2023
- 资助金额:30 万元
- 项目类别:地区科学基金项目
相似海外基金
Treatment of Youth Comorbid Conduct Problems and Depression
青少年共病行为问题和抑郁症的治疗
- 批准号:79892147989214
- 财政年份:2010
- 资助金额:$ 19.97万$ 19.97万
- 项目类别:
Treatment of Youth Comorbid Conduct Problems and Depression
青少年共病行为问题和抑郁症的治疗
- 批准号:82982598298259
- 财政年份:2010
- 资助金额:$ 19.97万$ 19.97万
- 项目类别:
Treatment of Youth Comorbid Conduct Problems and Depression
青少年共病行为问题和抑郁症的治疗
- 批准号:81141378114137
- 财政年份:2010
- 资助金额:$ 19.97万$ 19.97万
- 项目类别:
Clinical Trials Network, Ohio Valley Node
临床试验网络,俄亥俄谷节点
- 批准号:74983137498313
- 财政年份:2000
- 资助金额:$ 19.97万$ 19.97万
- 项目类别:
Clinical Trials Network, Ohio Valley Node
临床试验网络,俄亥俄谷节点
- 批准号:74146647414664
- 财政年份:2000
- 资助金额:$ 19.97万$ 19.97万
- 项目类别: