Cortical mechanisms mediating visual function and behavior

介导视觉功能和行为的皮质机制

• 批准号: 10665766
• 负责人: Nicholas J Priebe
• 金额: $ 52.67万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-01 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10665766
• 关键词: Accounting; Address; Affect; Anesthesia procedures; Animals; Attention; Behavior; Behavior Control; Behavioral; Biophysics; Cells; Cerebral cortex; Complement; Computer Models; Data Set; Detection; Esthesia; Event; Facial Expression; Future; Goals; Grant; Laboratories; Macaca; Measurement; Measures; Mediating; Membrane; Membrane Potentials; Modeling; Monitor; Monkeys; Motivation; Nature; Neurons; Noise; Output; Photic Stimulation; Physiological; Population; Primates; Property; Pupil; Recurrence; Research; Role; Sensory; Shapes; Signal Transduction; Source; Stimulus; Synapses; Techniques; Testing; Time; V1 neuron; Variant; Vision; Visual; Visual Perception; Whole-Cell Recordings; area striata; awake; behavioral response; computational platform; expectation; experimental study; individual response; insight; millimeter; neural; neural circuit; neural model; novel; originality; recurrent neural network; response; sample fixation; sensory cortex; visual control; visual stimulus; Accounting; Address; Affect; Anesthesia procedures; Animals; Attention; Behavior; Behavior Control; Behavioral; Biophysics; Cells; Cerebral cortex; Complement; Computer Models; Data Set; Detection; Esthesia; Event; Facial Expression; Future; Goals; Grant; Laboratories; Macaca; Measurement; Measures; Mediating; Membrane; Membrane Potentials; Modeling; Monitor; Monkeys; Motivation; Nature; Neurons; Noise; Output; Photic Stimulation; Physiological; Population; Primates; Property; Pupil; Recurrence; Research; Role; Sensory; Shapes; Signal Transduction; Source; Stimulus; Synapses; Techniques; Testing; Time; V1 neuron; Variant; Vision; Visual; Visual Perception; Whole-Cell Recordings; area striata; awake; behavioral response; computational platform; expectation; experimental study; individual response; insight; millimeter; neural; neural circuit; neural model; novel; originality; recurrent neural network; response; sample fixation; sensory cortex; visual control; visual stimulus

Project Summary Intracellular recording from sensory cortex provides a window into the synaptic inputs that shape spiking responses of individual cortical neurons, but until recently, this powerful technique has been limited to anesthetized animals. By combining the unique expertise from our laboratories, we have developed a novel technique that allow us to conduct, on a routine basis, reliable, whole-cell intracellular recording in primary visual cortex (V1) of awake, behaving macaque monkeys. We combine intracellular recording with an array of concomitant measurements that provide access to the state of the local network in which the neuron is embedded as well as to the internal state of the animal. Using these techniques, we have access to both subthreshold (membrane potentials, representing input) and suprathreshold (spikes, representing output) responses of individual cortical neurons, while also utilizing the precise control of visual stimulation and the subject’s behavioral state afforded by behaving primates. Our ability to perform intracellular recording in awake, behaving primates opens the door to addressing three fundamental questions with respect to the circuit-level mechanisms that mediate visual perception: (1) what are the nature, sources, and behavioral consequences of the large neural variability of sensory cortical neurons, (2) what is the contribution of internal state fluctuations to this variability, (3) what circuit models can account for the observed neural variability during spontaneous and evoked responses? To address these questions, in Aim 1 we will study the quantitative relationship between sub- and suprathreshold activity during spontaneous and stimulus-evoked responses in V1 of fixating monkeys. This will allow us to test the generality of previous findings from anesthetized animals. In Aim 2, we will examine the relationship between the activity of single V1 neurons and perceptual decisions in monkeys that are engaged in a demanding visual detection task. Specifically, we will examine how sub- and suprathreshold responses are altered by changing the attentional and motivational states under which the stimulus is presented. Finally, in Aim 3 we will test a novel set of circuit models that can account for our observed results and guide future experiments.

项目摘要 从感觉皮层中的细胞内记录提供了一个窗口，进入突触输入的窗口 单个皮质神经元的反应，但直到最近，这种强大的技术都仅限于 麻醉动物。通过结合实验室的独特专业知识，我们开发了一本小说 使我们常规地进行可靠的全细胞内记录的技术 醒着的视觉皮层（V1），表现出猕猴。我们将细胞内记录与一系列 伴随的测量可访问神经元所在的本地网络状态 嵌入和嵌入动物的内部状态。使用这些技术，我们可以访问 子阈值（膜电位，代表输入）和近路（尖峰，代表输出） 单个皮质神经元的反应，同时还利用视觉刺激的精确控制和 受试者的行为状态由行为私人提供。 我们在清醒中执行细胞内记录的能力，行为私人为解决三个 关于介导视觉感知的电路级机制的基本问题：（1）什么 感觉皮质的大神经变异性的性质，来源和行为后果是 神经元，（2）内部状态波动对这种变异性的贡献是什么，（3）什么电路模型 可以考虑在赞助和诱发反应期间观察到的神经变异性吗？ 为了解决这些问题，在目标1中，我们将研究子和子之间的定量关系 在固定猴子的V1中，自发和刺激诱发的反应期间的上空活动。这 将使我们能够测试麻醉动物的先前发现的普遍性。在AIM 2中，我们将检查 单个V1神经元的活动与猴子的感知决定之间的关系 从事苛刻的视觉检测任务。具体来说，我们将研究子和跨股权 通过改变刺激的注意力和动机状态来改变反应 提出。最后，在AIM 3中，我们将测试一组新型的电路模型，这些模型可以解释我们观察到的结果 并指导未来的实验。