Core B -- BioCore

核心B——BioCore

• 批准号: 10559174
• 负责人: CHRISTOPHER L COE
• 金额: $ 63.88万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-25 至 2028-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10559174
• 关键词: Aliquot; Alzheimer' s disease related dementia; Alzheimer' s disease risk; Antioxidants; Archives; Biological; Biological Assay; Biological Markers; Blood; Blood Tests; Body Fluids; Catecholamines; Cell Separation; Cells; Clinical; Code; Collection; Consult; Consultations; Data; Data Files; Development; Education; Educational workshop; Endocrine; Ensure; Fluorescence; Freezing; Future; Gene Expression; Genetic Transcription; Health; Hematology; High Density Lipoproteins; Home; Hormones; Hydrocortisone; Immunoassay; Immunology procedure; Inflammatory; Information Dissemination; Infrastructure; Institutional Review Boards; Laboratories; Link; Liquid substance; Location; Mass Spectrum Analysis; Measures; Mentors; Metabolic; Methodology; Methods; Mission; Monitor; Nitrogen; Paper; Participant; Pathway interactions; Performance; Peroxidases; Preparation; Process; Protocols documentation; Public Domains; Publications; Quality Control; Records; Reporting; Research Personnel; Resources; Risk; Role; Saliva; Salivary; Sampling; Ships; Site; Specimen; Spottings; Standardization; Sum; System; Test Result; Testing; Time; Workload; biological specimen archives; biomarker panel; bone turnover; chemokine; cytokine; data sharing; diaries; experience; liquid chromatography mass spectrometry; meetings; operation; pedagogy; psychosocial; quality assurance; repository; transmission process; urinary

ABSTRACT – BIOCORE To obtain the full panel of biomarkers in the MIDUS project, collection and analytic efforts must be coordinated. The BioCore (B) thus fulfills an essential role in MIDUS by providing centralized, reliable testing, processing, and storage of biospecimens (i.e., hematological, endocrine, inflammatory, metabolic, bone turnover, antioxidant, gene expression). All tests are performed by single laboratories to ensure consistent results over time. Core B also assists in the standardization of protocols (i.e., identical collection and processing SOPs) and monitors shipment integrity and testing at the analytic laboratories over time (i.e., Quality Control). Core B also has the primary responsibility for storing aliquots from new samples and maintaining a complete set of archival specimens in ultracold freezers. In addition to overseeing all testing, the BioCore conducts assays and transports the samples that are tested at local clinical labs in Madison, WI. A centralized Core operation is essential to ensure uniform entry of test results into a single record system. One corollary of this administrative function is reviewing the reliability of data entry before transmission of results for dissemination and combining with demographic/psychosocial data. Core B also has several advisory and pedagogical functions, which includes providing “norms” for test results, meeting with investigators to advise on analyses, and conducting educational workshops on the use and meaning of biological measures at MIDUS meetings. The future performance of the BioCore will be facilitated by the extensive experience gained during the prior MIDUS periods. The panel of biomarkers going forward is similar to the panels employed previously with some new additions. The BioCore has previously assisted in establishing new methodologies (e.g., multi-cytokine arrays, an assay of HDL anti-oxidant activity) and will also oversee new analyte testing related to risk for Alzheimer’s Disease and Related Dementias (ADRD) linked with the Ancillary U01 application. To highlight the BioCore’s level of experience, over 100,000 specimens have been tested in the prior periods. Processing of blood and/or cells for gene expression is also routine for Dr. Coe’s (PI, BioCore) laboratory. Dr. Coe has over 3 decades of prior experience with endocrine and immune assays, including with many body fluids, different assay platforms and cell separation protocols. In sum, Core B provides the infrastructure and required resources to ensure high quality and reliable biomarker assessments in MIDUS.

摘要 – 生物核心 为了获得 MIDUS 项目中的全套生物标志物，必须协调收集和分析工作。 因此，BioCore (B) 通过提供集中、可靠的测试、处理、 和生物样本的储存（即血液学、内分泌、炎症、代谢、骨转换、 所有测试均由单一实验室进行，以确保结果一致。 核心 B 还有助于协议的标准化（即相同的收集和处理 SOP）和 随着时间的推移，监控分析实验室的运输完整性和测试（即质量控制核心 B）。 主要负责存储新样品的等分试样并维护一套完整的档案 BioCore 除了监督所有测试外，还进行分析和分析。 运输在威斯康星州麦迪逊市当地临床实验室进行测试的样本 集中化的核心操作是。 对于确保将测试结果统一输入单一记录系统至关重要。这一管理的一个必然结果。 功能是在传输结果进行传播和合并之前审查数据输入的可靠性 具有人口/心理社会数据的核心 B 还具有多种咨询和教学功能。 包括提供测试结果的“规范”，与研究人员会面以就分析提出建议，以及进行 在 MIDUS 会议上举办关于生物措施的使用和意义的教育研讨会。 BioCore 的性能将通过之前 MIDUS 期间获得的丰富经验得到促进 未来的生物标记物组与之前使用的一些新的组相似。 BioCore 之前曾协助建立新的方法（例如，多细胞因子阵列、 HDL 抗氧化活性测定），还将监督与阿尔茨海默病风险相关的新分析物测试 疾病及相关痴呆症 (ADRD) 与辅助 U01 应用程序相关 强调 BioCore 的功能。 根据经验水平，在之前的血液和/或处理过程中已经测试了超过 100,000 个样本。 细胞进行基因表达也是 Coe 博士（PI，BioCore）实验室的常规工作。Coe 博士拥有 3 多年的研究经验。 具有内分泌和免疫检测经验，包括许多体液、不同的检测平台 总之，Core B 提供了基础设施和所需的资源，以确保高水平。 MIDUS 中质量可靠的生物标志物评估。