Imaging Structure and Function of Photoreceptors

感光器的成像结构和功能

基本信息

批准号：
10522431
负责人：
Donald T Miller
金额：
$ 57.7万
依托单位：
TRUSTEES OF INDIANA UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-09-01 至 2026-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10522431
关键词：
3-Dimensional Affect Algorithms Anatomy Blindness Cells Clinic Color Color Visions Complex Cone Custom Data Detection Disease Early Diagnosis Early treatment Electrophysiology (science)Electroretinography Eye Genotype Goals Gold Grant Heterozygote Human Image Individual Inherited Investigation Laboratories Length Light Measurement Measures Mediating Methods Modality Monitor Mosaicism Optical Coherence Tomography Optical Methods Optics Outcome Patients Phase Phenotype Photoreceptors Physiological Physiological Processes Process Property Psychophysics Red-Green Color Blindness Research Resolution Retina Retinal Cone Retinal Degeneration Retinitis Pigmentosa Safety Savings Specificity Structure System Techniques Technology Testing Tissues Vision X Inactivation adaptive optics base cell type cellular imaging gene replacement therapy genetic testing image registration improved in vivo instrumentation light scattering live cell imaging relating to nervous system response retinal rods stem cell therapy therapy development tool treatment trial

项目摘要

Project Summary/Abstract Human vision starts when photoreceptors collect and respond to light. Normal photoreceptor function is essential for normal vision, yet techniques to assess these processes in vivo are limited. New optical modalities that are rapid, specific, and non‐invasive promise to greatly expand our capability to monitor more accurately and completely photoreceptors. This study takes advantage of unique adaptive‐optics OCT instrumentation developed in my laboratory in conjunction with custom algorithms for sub‐cellular image registration and phase‐sensitive detection to measure anatomical and physiological properties of individual photoreceptors. We will use this technique to investigate three specific aims that quantify the spectral sensitivity profiles of photoreceptors, the expression of photoreceptor spectral types in color vision deficiencies, and the temporal dynamics of photoreceptor loss in retinitis pigmentosa patients. The long term goal of this research is to establish high resolution, high specificity optical techniques as valid tools for probing structure and physiologic processes of the retina at the cellular scale. The resulting ability to study cells in vivo will improve early detection of and treatment monitoring for diseases that impact the retina.

项目概要/摘要当光感受器收集光并对光做出反应时，人类的视觉就开始了。正常的光感受器功能是正常的。对于正常视力至关重要，但体内评估这些过程的技术是有限的。快速、具体和非侵入性的承诺将大大扩展我们更准确监测的能力和完全的光感受器。这项研究利用了我的实验室开发的独特的自适应光学 OCT 仪器与亚细胞图像配准和相敏检测的定制算法相结合我们将使用这种技术来测量单个光感受器的解剖学和生理学特性。研究量化光感受器光谱灵敏度分布的三个具体目标，色觉缺陷中的感光器光谱类型，以及色觉缺陷中感光器损失的时间动态这项研究的长期目标是建立高分辨率、高特异性的视网膜色素变性患者。光学技术作为探测细胞视网膜结构和生理过程的有效工具由此产生的体内细胞研究能力将改善早期检测和治疗监测。影响视网膜的疾病。