Imaging Structure and Function of the Photoreceptor-RPE-Choriocapillaris Complex

光感受器-RPE-脉络膜毛细血管复合物的成像结构和功能

• 批准号: 9979890
• 负责人: Donald T Miller
• 金额: $ 45.59万
• 依托单位: TRUSTEES OF INDIANA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2022-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9979890
• 关键词: 3-Dimensional; Address; Age related macular degeneration; Animal Model; Architecture; Atrophic; Autopsy; Biological; Biological Markers; Blindness; Cell physiology; Cells; Cellular Structures; Cessation of life; Clinical; Complex; Cone; Disease; Diurnal Rhythm; Drusen; Early Diagnosis; Early treatment; Electrophysiology (science); Eye; Eye diseases; Goals; Grain; Health; Hereditary Disease; Human; Image; Individual; Inherited; Intervention; Kinetics; Laboratories; Length; Light; Measurement; Measures; Modality; Monitor; Mosaicism; Optical Coherence Tomography; Optical Methods; Optics; Patients; Phagocytes; Phase; Photoreceptors; Physiological Processes; Process; Property; Reporting; Research; Resolution; Retina; Retinal Diseases; Retinal Dystrophy; Retinal Photoreceptors; Retinitis Pigmentosa; Rod; Sampling; Site; Specificity; Structure; Structure of retinal pigment epithelium; Techniques; Technology; Testing; Vision; adaptive optics; base; cell type; circadian; human imaging; improved; in vivo; instrumentation; interest; light scattering; phenotypic biomarker; photoreceptor disc; retinal imaging; tool

Project Summary/Abstract Human vision starts when photoreceptors collect and respond to light. Photoreceptors do not function in isolation though, but share close interdependence with neighboring photoreceptors, retinal pigment epithelium (RPE) cells, and choriocapillaris (CC). These close interactions underlie normal function of photoreceptors, but also the entire photoreceptor-RPE-CC complex, the primary site of most retinal dystrophies including age-related macular degeneration (AMD) and inherited diseases such as retinitis pigmentosa (RP). Techniques to assess this complex in vivo, however, are limited. New optical modalities that are rapid, specific, and non-invasive hold the promise of greatly expanding our capability to monitor more accurately and completely the photoreceptor-RPE-CC complex. This study takes advantage of unique AO and OCT instrumentation developed in my laboratory for sampling rapidly and reproducibly volume regions of the photoreceptor-RPE-CC complex at the cellular level. We will use MHz AO-OCT in conjunction with sub-cellular 3D registration and phase techniques that we have developed that are sensitive to optical length changes as small as 45 nm. We will use these techniques to investigate three specific aims: (1) determine properties of photoreceptor disc shedding, (2) evaluate disruption in the cellular surround of drusen, an early indicator of AMD, and (3) measure cell loss dynamics in RP.

项目概要/摘要 当光感受器收集光并对光做出反应时，人类的视觉就开始了。光感受器不发挥作用 虽然隔离，但与邻近的光感受器、视网膜色素密切相关 上皮细胞 (RPE) 和脉络膜毛细血管细胞 (CC)。这些密切的相互作用是正常功能的基础 光感受器，而且还包括整个光感受器-RPE-CC复合体，大多数视网膜的主要部位 营养不良，包括年龄相关性黄斑变性 (AMD) 和视网膜炎等遗传性疾病 色素变性（RP）。然而，体内评估这种复合物的技术是有限的。新的光学模式 快速、特异性和非侵入性有望大大扩展我们监测更多内容的能力 准确、完整地构建光感受器-RPE-CC复合物。 本研究利用我实验室开发的独特 AO 和 OCT 仪器进行采样 在细胞水平上快速且可重复地测量光感受器-RPE-CC复合物的体积区域。我们将 将 MHz AO-OCT 与我们拥有的亚蜂窝 3D 配准和相位技术结合使用 开发出对小至 45 nm 的光学长度变化敏感的技术。我们将使用这些技术来 研究三个具体目标：(1) 确定感光盘脱落的特性，(2) 评估 玻璃疣（AMD 的早期指标）细胞周围的破坏，以及 (3) 测量细胞损失动态 RP。