Imaging spatial and temporal dynamics of retinal ganglion cells

视网膜神经节细胞的时空动态成像

基本信息

批准号：
9897531
负责人：
Donald T Miller
金额：
$ 40.97万
依托单位：
TRUSTEES OF INDIANA UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-04-01 至 2023-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9897531
关键词：
3-Dimensional Address Affect Aging Alzheimer&apos s Disease Amacrine Cells Animals Atrophic Autopsy Axon Biological Markers Blindness Blood capillaries Brain Cell Count Cell Fraction Cells Clinical Contrast Media Cross-Sectional Studies Detection Disease Dropout Early Diagnosis Early treatment Electrophysiology (science)Exhibits Eye Ganglion Cell Layer Glaucoma Goals Health Histologic Histology Human Image Individual Laboratories Literature Location Longitudinal Studies Measurement Measures Methods Microglia Modality Monitor Motion Multiple Sclerosis Nerve Fibers Neural Retina Neurodegenerative Disorders Neurons Optical Coherence Tomography Optical Methods Optics Organelles Parkinson Disease Pathology Phase Photoreceptors Physiological Processes Population Process Property Research Resolution Retina Retinal Ganglion Cells Risk Role Signal Transduction Specificity Structure Techniques Technology Testing Thick Three-Dimensional Imaging Time Uncertainty Variant Vision Visual Fields adaptive optics age related base cell motility cell type cellular imaging density fovea centralis ganglion cell image registration improved in vivo instrumentation interest morphometry neural circuit neuron loss neuronal cell body normal aging novel optic nerve disorder preference retinal neuron tool transmission process visual performance

项目摘要

Project Summary/Abstract Ganglion cells (GCs) and other inner retinal neurons are fundamental to retinal neural circuitry, processing photoreceptor signals relayed from intermediate neurons for transmission to the brain. Yet, much remains unknown about their role in vision and their vulnerability to disease leading to blindness. GCs in particular are lost to neurodegenerative disorders such as glaucoma, Alzheimer’s disease, Parkinson’s disease, and multiple sclerosis. We also know from histology that a small fraction of these cells die each year as part of the normal aging process. Unfortunately, techniques to assess population loss and general health of GCs and other inner retinal neurons in vivo are limited. New optical modalities that are rapid, specific, and non-invasive promise to greatly enhance our ability to monitor the spatial and temporal dynamics of inner retinal neurons. This study takes advantage of unique optical instrumentation developed in my laboratory that combines adaptive optics and optical coherence tomography to achieve cellular-level 3D imaging of the living human retina. We will use this technique to investigate three specific aims that quantify the spatial properties of inner retinal neurons and their change in aging and glaucoma. The long term goal of this research is to establish high resolution, high specificity optical techniques as valid tools for probing structure and physiologic processes of the retina at the cellular scale. The resulting ability to study cells in vivo will improve early detection of and treatment monitoring for diseases that impact the retina.

项目概要/摘要神经节细胞 (GC) 和其他视网膜内神经元是视网膜神经回路、处理信息的基础然而，光感受器信号从中间神经元传递到大脑。尚不清楚它们在视力中的作用以及它们对导致失明的疾病的脆弱性。因青光眼、阿尔茨海默病、帕金森病等神经退行性疾病而丢失我们还从组织学中得知，每年都有一小部分细胞死亡，作为多发性硬化症的一部分。不幸的是，评估GC和其他群体损失和总体健康状况的技术。体内视网膜神经元的快速、特异性和非侵入性的新光学方式是有限的。有望大大增强我们监测视网膜内部神经元的空间和时间动态的能力。这项研究利用了我实验室开发的独特光学仪器，该仪器结合了自适应光学和光学相干断层扫描实现活体细胞级 3D 成像我们将使用这种技术来研究量化内部空间特性的三个具体目标。视网膜神经元及其在衰老和青光眼中的变化这项研究的长期目标是建立高水平。分辨率、高特异性光学技术作为探测结构和生理过程的有效工具由此产生的在体内研究细胞的能力将改善早期检测和诊断。用于监测影响视网膜的疾病的治疗。