Charge Transfer Study of DNA/MoS2 interface

DNA/MoS2界面的电荷转移研究

• 批准号: 10514726
• 负责人: Mohtashim H Shamsi
• 金额: $ 42.95万
• 依托单位: SOUTHERN ILLINOIS UNIVERSITY CARBONDALE
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10514726
• 关键词: Address; Affect; Affinity; Area; Basic Science; Behavior; Binding; Biomedical Research; Biomedical Technology; Characteristics; Charge; Chemistry; DNA; DNA Sequence; DNA sequencing; DNA-Binding Proteins; Data; Detection; Development; Devices; Disease; Disulfides; Electron Transport; Future; GTP-Binding Protein alpha Subunits, Gs; Genes; Goals; Growth; Human; Individual; Investigation; Label; Length; Measurement; Microscopy; Molecular Conformation; Molybdenum; Monitor; Mutation; Nucleotides; Pathologic; Patients; Persons; Positioning Attribute; Proteins; Public Health; Research; Research Personnel; Resistance; Science; Signal Transduction; Single Nucleotide Polymorphism; Students; Supervision; Surface; Techniques; Technology; Testing; Theoretical Studies; Transducers; Trinucleotide Repeats; base; design; detection limit; detection platform; diagnostic technologies; flexibility; genetic testing; graduate student; improved; interfacial; materials science; mutant; nanomaterials; nanopore; nanosheet; nucleobase; tool; two-dimensional; undergraduate student; van der Waals force

Project Summary Expansion of tandem DNA repeats cause more than forty genetically transferrable disorders, which affect 4 million people every year. Current state-of-the-art diagnostic technologies for genetic testing for length mutations have their own limitations such as clogging of protein nanopores, requiring labelling steps, frequent false positive/negative results, or short basepair read length. To overcome the limitations that hamper the current biomedical science, there is a critical need to develop new platforms founded on thorough basic science. This AREA proposal involving mainly undergraduate researchers investigates intrinsic character of tandem DNA repeats interfaced with MoS2 surfaces that may manifest into label-free sensing platform for repeat mutations in future. The PI hypothesizes that DNA repeats can produce sequence- and length-dependent charge transfer signals due to the differential affinity of nucleobases for two-dimensional materials, i.e. molybdenum disulfide (MoS2). This is the critical piece of information needed to confirm through a rigorous study. Based on encouraging preliminary results, current project is designed to fundamentally investigate DNA/MoS2 interfaces in detail by electrochemical and surface probe microscopy techniques. In the specific aims, the PI plans to (1) investigate sequence-dependent charge transport at TNR/2D nanomaterials interface, and (2) investigate their behavior with respect to sequence length. The results will also be compared with concentration and conformations effects on the charge transfer character. This AREA proposal will expose the undergraduate researchers to high-quality research in surface chemistry and materials science, which has ultimate application in biomedical research to improve public health. Upon completion, we will be in better position to apply DNA/2D materials for selective and sensitive detection of repeat mutations, which will ultimately improve the lives of millions of individuals.

项目摘要 串联DNA重复的扩展会导致40多个遗传转移疾病， 每年影响400万人。遗传的当前最新诊断技术 长度突变的测试具有其自身的局限性，例如蛋白质纳米孔的堵塞，需要 标记步骤，频繁的假阳性/负面结果或短基础读取长度。克服 妨碍当前生物医学科学的局限性，建立新平台的迫切需要 建立在彻底的基础科学上。该领域的建议主要涉及本科研究人员 研究串联DNA重复与MOS2表面连接的固有特征 进入未来重复突变的无标签传感平台。 PI假设DNA重复可以产生序列和长度依赖性电荷 由于核碱基对二维材料的差异亲和力的转移信号，即 钼二硫化物（MOS2）。这是通过 严格的研究。基于鼓励初步结果，当前的项目旨在从根本上 通过电化学和表面探针显微镜详细研究DNA/MOS2界面 技术。在具体目的中，PI计划（1）研究序列依赖性电荷运输 TNR/2D纳米材料的界面，（2）研究其在序列长度方面的行为。这 结果还将与浓度和构象对电荷转移的影响进行比较 特点。 该领域的建议将使本科研究人员接受高质量研究 表面化学和材料科学，在生物医学研究中最终应用 改善公共卫生。完成后，我们将在更好的位置应用DNA/2D材料 对重复突变的选择性和敏感检测，最终将改善数百万的生活 个人。