tRNA modifications as critical components of insect blood feeding and reproduction

tRNA 修饰作为昆虫血液喂养和繁殖的关键组成部分

• 批准号: 10648600
• 负责人: Josh B. Benoit
• 金额: $ 20.25万
• 依托单位: UNIVERSITY OF CINCINNATI
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-03-16 至 2025-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10648600
• 关键词: Area; Arthropods; Bacteria; Biochemical; Biological; Biology; Blood; Chemicals; Codon Nucleotides; Culicidae; Data; Data Set; Enzymes; Eukaryota; Event; Fat Body; Female; Future; Gene Expression Profile; Gene Expression Profiling; Generations; Genes; Genetic Transcription; Goals; Hour; Human; Insect Vectors; Insecta; Insecticide Resistance; Life; Life Cycle Stages; Link; Methods; Midgut; Modification; Nucleosides; Nutrient; Organ; Organism; Osmosis; Output; Oxidative Stress; Partner in relationship; Pattern; Phenotype; Physiological; Physiology; Play; Population; Process; Production; Protein Biosynthesis; Publishing; RNA; Reproduction; Research; Resources; Ribonucleosides; Role; Sampling; Sex Differences; Structure; System; Testing; Time; Tissue-Specific Gene Expression; Tissues; Transcript; Transfer RNA; Untranslated RNA; Whole Organism; Yeasts; climate change; disease transmission; egg; experimental study; feeding; male; novel; pathogen; prevent; reproductive; reproductive organ; response; sex; stress tolerance; transmission process; vector; vector control

Project summary Transfer RNAs (tRNA) represent one of the most abundant forms of RNA and are required for protein synthesis. Chemically modified ribonucleosides in tRNAs play critical roles in aspects that range from decoding codons to stress tolerance. Limited studies for metazoans, specifically in blood feeding arthropods, have been conducted on tRNAs and their modifications. The goal of these studies is to provide a characterization of the tRNA pool and modifications in blood feeding insects. These changes will be examined during blood feeding, reproduction, and mating at the whole organism and tissue levels. Our studies are supported by the following: 1) preliminary results from limited samples, which identified and quantified tRNA modifications, that suggest there are differences between blood feeding and sexes; and 2) Re-analysis of previously published data sets indicate expression patterns of genes associated with tRNA modification vary following a blood meal and between sexes. This study has two specific aims: Specific Aim 1. Characterize transfer RNA modifications in insects between sexes and during blood feeding. Specific Aim 2. Investigate the role of transfer RNA modifications during blood feeding and reproduction. Upon completion of this proposed research, we will establish tRNA expression and modification profiles for multiple blood feeding species in relation to reproduction and blood feeding. The role of several modifications will be elucidated by the use of targeted reduction of specific tRNA modifying enzymes. These proposed studies will be the first to extensively examine tRNA expression and modification patterns in blood feeding insects. Our results will provide the groundwork for future, novel tRNA-based studies of blood feeding insects such as identifying tRNA modifications critical to vector survival as climate change progresses and the dynamics between tRNA expression and modifications during pathogen transmission.

项目概要 转移 RNA (tRNA) 是最丰富的 RNA 形式之一，是蛋白质合成所必需的。 tRNA 中经过化学修饰的核糖核苷在从解码密码子到 压力耐受力。对后生动物，特别是吸血节肢动物的研究有限 tRNA 及其修饰。这些研究的目的是提供 tRNA 库的表征以及 食血昆虫的改变。这些变化将在血液喂养、繁殖和发育过程中进行检查。 在整个生物体和组织水平上交配。我们的研究得到以下支持：1）初步结果 来自有限的样本，这些样本鉴定并量化了 tRNA 修饰，这表明存在差异 献血和性别之间； 2) 对先前发布的数据集进行重新分析表明表达 与 tRNA 修饰相关的基因模式在吸血后和性别之间会有所不同。 这项研究有两个具体目标： 具体目标 1. 表征昆虫在性别之间和吸血期间的转移 RNA 修饰。 具体目标 2. 研究转移 RNA 修饰在血液喂养和繁殖过程中的作用。 完成这项拟议的研究后，我们将建立 tRNA 表达和修饰谱 与繁殖和血液喂养有关的多种血液喂养物种。几处修改的作用 将通过使用特定 tRNA 修饰酶的靶向还原来阐明。这些拟议的研究 将是第一个广泛研究吸血昆虫中 tRNA 表达和修饰模式的人。我们的 结果将为未来基于 tRNA 的吸血昆虫的新型研究奠定基础，例如 随着气候变化的进展以及两者之间的动态变化，识别对载体生存至关重要的 tRNA 修饰 病原体传播过程中 tRNA 的表达和修饰。