Neural Circuits Underlying Multisensory Control of Orientation in Drosophila

果蝇方向多感官控制的神经回路

• 批准号: 10647673
• 负责人: Katherine Nagel
• 金额: $ 38.17万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-07-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10647673
• 关键词: Adult; Area; Behavior; Behavioral; Behavioral Paradigm; Brain; Brain region; Cell model; Cells; Complex; Coupled; Cues; Data; Detection; Disease; Drosophila genus; Ear; Electrophysiology (science); Environmental Wind; Exhibits; Experimental Models; Eye; Flying body movement; Functional Imaging; Genetic; Genetic Screening; Goals; Grant; Human; Literature; Location; Logic; Memory; Modality; Modeling; Motion; Motor; Movement; Neurons; Nose; Odors; Pathway interactions; Population; Role; Sensory; Shapes; Signal Transduction; Stimulus; Stream; Structure; Sum; Testing; Vertebrates; Visual; Walking; behavioral outcome; flexibility; fly; insight; model organism; motor control; multimodality; multisensory; neural circuit; novel; optogenetics; response; sensory integration; sensory stimulus; transmission process; visual processing; visual stimulus

Project Summary! How the brain combines sensory stimuli from different modalities to guide behavior is currently not understood at a single-cell level. Here we propose to use the model organism Drosophila to investigate the neural circuit basis of multi-sensory integration in the context of two specific orientation behaviors developed in our lab. In one paradigm, odor switches the orientation of walking flies to a wind (mechanosensory) cue: flies orient downwind in the absence of odor and upwind in the presence of an attractive odor. In the second paradigm, an aversive wind stimulus and attractive visual stimulus sum to guide orientation of a tethered flying fly. We have discovered a novel mechanosensory pathway that computes wind direction from movements of the fly's antennae, and transmits this information to the central complex, a region of the brain implicated in navigation. Combined with information from the literature and preliminary data from our own lab, this suggests a model for how different sensory streams might be aligned and integrated within the central complex. We propose to use optogenetic activation, chemogenetic silencing, whole-cell electrophysiology, and functional imaging to test the role of candidate central complex neurons in multi-sensory control of orientation. We will additionally test the alternative hypothesis that multi-sensory integration in descending neurons is required for the two behaviors. This study will provide a comprehensive cellular-level account of how sensory streams are combined to control the orientation of a model organism.!

项目摘要！ 当前，大脑如何结合不同方式引导行为的感觉刺激 在单细胞级别不了解。在这里，我们建议使用模型有机体果蝇 在两个特定的背景下研究多感官整合的神经回路基础 我们实验室发展的方向行为。在一个范式中，Otor切换了 在没有气味的情况下，步行浮游（机械感应）提示：沿阵风向下顺风 在有吸引人的气味的情况下向上风。在第二个范式中，厌恶风 刺激和有吸引力的视觉刺激总和，以指导束缚的平流的方向。我们有 发现了一种新型的机理感觉途径，该途径从运动中计算风向 频线的触角，并将这些信息传输到中心综合体，该区域是 大脑在导航中实现。结合文献和初步的信息 来自我们自己实验室的数据，这提出了一个模型，以了解不同的感觉流 对齐并整合在中央建筑群中。我们建议使用光遗传激活， 化学发生沉默，全细胞电生理学和功能成像，以测试 候选中央复合神经元在多敏化方向控制中。我们还将 测试需要在降降神经元中多敏整合的替代假设 对于这两种行为。这项研究将为如何提供有关如何 感觉流组合以控制模型生物的方向。