The Neural Circuit Basis of Olfactory Navigation in Adult Drosophila

成年果蝇嗅觉导航的神经回路基础

• 批准号: 10447440
• 负责人: Katherine Nagel
• 金额: $ 171.72万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-03-15 至 2025-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10447440
• 关键词: Adult; Algorithms; Anatomy; Animals; Behavior; Behavioral; Biological Assay; Brain; Cells; Complex; Computer Models; Cues; Data; Drosophila genus; Electrophysiology (science); Environment; Environmental Wind; Experimental Models; Food; Genetic; Goals; Image; Invertebrates; Label; Link; Location; Measurement; Measures; Memory; Modeling; Motor; Movement; Neurons; Nutritional; Odors; Output; Play; Role; Sensory; Series; Signal Transduction; Source; Stimulus; Testing; Translating; Vertebrates; base; behavioral response; connectome; connectome data; experimental study; neural circuit; optogenetics; programs; response; source localization; tool; two-photon

PROJECT SUMMARY In order to forage effectively for food, the brain must integrate innate and learned information about the value of different food odors and use this information to select navigational motor programs. Although a great deal is known about how the brain computes the value of odor stimuli, how it uses this information to guide navigation remains mysterious. Here we propose to investigate the role of a conserved navigation center, the fan-shaped body (FB), in olfactory navigation. The FB receives a large number of inputs that are anatomically downstream of innate and learned olfactory processing centers. We have identified several lines labeling such inputs that respond to attractive odor and drive upwind orientation when activated. Here we will use whole-cell electrophysiology and imaging to investigate the representation of innate and learned odor value signals in FB input neurons. By combining these functional data with detailed connectomic information, we will generate quantitative models that relate sensory representations to anatomical connectivity. Next we will use both synchronous and asynchronous recordings to measure and model the relationship between FB neuron activity and navigational action selection. Finally, we will develop a new optogenetic plume assay that will allow us to determine the role of FB neurons in different aspects of odor plume navigation. Together these experiments and models will generate testable predictions about how the organization of central navigation circuits supports a critical goal-directed behavior, odor-guided food search.

项目摘要 为了有效觅食，大脑必须整合先天和学习的信息 不同食物气味的价值，并使用此信息选择导航运动程序。虽然 关于大脑如何计算气味刺激的价值，如何使用此信息的知识很广 指导导航仍然是神秘的。在这里，我们建议调查保守的作用 导航中心，风扇形身体（FB），嗅觉导航。 FB接收大量 在解剖学上是先天和学识渊博的嗅觉加工中心的输入。我们有 确定了几行标记此类输入的行 激活时。在这里，我们将使用全细胞电生理学和成像来研究 FB输入神经元中先天和学习的气味价值信号的表示。通过结合这些 功能数据带有详细的连接信息，我们将生成相关的定量模型 解剖连通性的感官表示。接下来，我们将同时使用同步和异步 记录以测量和建模FB神经元活动与导航作用之间的关系 选择。最后，我们将开发出一种新的光遗传羽状测定法，这将使我们能够确定角色 在气味羽流导航各个方面的FB神经元。这些实验和模型一起将 关于中央导航电路的组织如何支持关键的可测试预测 目标指导的行为，气味引导的食物搜索。