Ethnopharmacology of plants used by Haitians in New York City forwomen’s health

纽约市海地人使用的植物对女性健康的民族药理学

基本信息

批准号：
10640068
负责人：
Ella Vardeman
金额：
$ 4.35万
依托单位：
CUNY GRADUATE SCH AND UNIV CTR
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-06-01 至 2025-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10640068
关键词：
Address Anti-Bacterial Agents Antimicrobial Effect Back Bacteria Bixa Botanica Botanicals COVID-19 pandemic Caribbean region Chemicals Cities Coculture Techniques Collaborations Commerce Communities Country Cross-Cultural Comparison Data Deposition Disease Effectiveness Environment Ethnobotany Ethnopharmacology Evaluation Feedback Fractionation Future Gardnerella vaginalis Growth Gynecologic Haitian Health Home Immigrant In Vitro Infection International Interview Investigation Jatropha Knowledge Laboratories Laboratory Study Lactobacillus Literature Marketing Measures Medical Medical Students Medicinal Plants Medicine Methodology Methods Monograph Natural Products New York New York City Pathogenicity Perception Persons Phytochemical Plant Extracts Plant Leaves Plants Population Preparation Prickly Poppy Property Public Health Reporting Research Self Medication Sexually Transmitted Diseases Specimen Statistical Data Interpretation Surveys Taxonomy Testing Tissues Traditional Medicine United Nations Vagina Validation Variant Woman Women&apos s Health World Health Organization antimicrobial chemical fingerprinting cost cultural competence ethnomedicine healing improved insight member metabolomics microbiota outreach pathogenic bacteria pathogenic microbe programs vaginal infection vaginal microbiota

项目摘要

Proposal Abstract Approximately 55% of the world’s population lives in cities, and the United Nations estimates that this number will rise to 68% by 2050. Urban ethnobotany, the study of people’s traditional knowledge and use of plants in cities, is increasingly important as people adapt their cultural traditions to changing environments. In New York City (NYC), there have been several urban ethnobotany investigations with Caribbean immigrant groups. However, there has yet to be an ethnobotanical survey that documents and botanically authenticates the medicinal plants used in the Haitian community. Previous research in collaboration with women from the Caribbean in NYC has identified plant species sold in Caribbean shops that have documented use for the treatment of vaginal infections, including sexually transmitted infections and intravaginal cleansing, grouped here as gynecological conditions. Several popular Caribbean plants, such as Jatropha curcas, Argemone mexicana, and Bixa orellana, have recorded medicinal uses in Haitian ethnomedicine as well. These species, while well- studied for their antibacterial properties, have not yet been analyzed for their effects on pathogenic bacteria in the vagina or the protective vaginal microbiota. Ideally, plants used to treat these conditions would have bioactivity against harmful bacteria with minimal disruption to the beneficial vaginal microflora. Natural product research that considers interactions with pathogenic and beneficial bacteria, as well as the compounds responsible, is on the forefront of addressing efficacy of medicinal plants for women’s health. I hypothesize that traditional preparations of the identified species will vary in inhibition of the growth of pathogenic (Gardnerella vaginalis) and beneficial (Lactobacillus) vaginal bacterial due to differences in specialized metabolites. Furthermore, I hypothesize that there will be similarities in plants and women’s health practices between the Haitian and other Caribbean communities in NYC, due to the shared flora in the Caribbean. The selected plant species will be analyzed for their effects on disease-causing bacteria, Gardnerella vaginalis as well as on prominent beneficial Lactobacillus species found in the vagina. Plant extracts will also be compared to determine variation in the composition of bioactive phytochemicals across preparations of different tissues, using LC-MS- qToF and subsequent multivariate statistical analysis. An ethnobotanical survey with the Haitian community will be used to further identify additional plant species used to treat gynecological conditions by Caribbean women for continued laboratory studies. This data will enable cross-cultural comparisons with other Caribbean communities in NYC, and will also assess how the COVID-19 pandemic has influenced NYC Haitian women in their use of medicinal plants for women’s health. Antibacterial natural products from plants that leave the vaginal microflora intact may provide low-cost and culturally-appropriate solutions for ongoing treatment of gynecological conditions.

提案摘要世界上大约 55% 的人口居住在城市，联合国估计这个数字到 2050 年将上升到 68%。城市民族植物学，研究人们在城市中的传统知识和植物使用在纽约，随着人们使自己的文化传统适应不断变化的环境，城市变得越来越重要。纽约市，已经对加勒比移民群体进行了几项城市民族植物学调查。然而，还没有一项民族植物学调查来记录和植物学上验证先前与海地妇女合作进行的药用植物研究。纽约市的加勒比地区已确定在加勒比地区商店出售的植物物种，这些植物物种已记录用于阴道感染的治疗，包括性传播感染和阴道内清洁，在此分组作为妇科疾病，几种流行的加勒比植物，如麻疯树、墨西哥银杏树、和 Bixa orellana，在海地民族医学中也记录了这些物种的药用用途。研究了其抗菌特性，但尚未分析其对病原菌的影响理想情况下，用于治疗这些病症的植物应该具有保护性阴道微生物群。对有害细菌具有生物活性，对有益阴道微生物群的破坏最小。考虑与致病菌和有益菌以及化合物的相互作用的研究负责，处于解决药用植物对女性健康功效的最前沿。已识别物种的传统制剂在抑制致病菌（加德纳菌）生长方面会有所不同由于特殊代谢物的差异，阴道细菌）和有益（乳酸菌）阴道细菌。此外，我认为不同国家之间在植物和女性健康实践方面会有相似之处。海地和纽约的其他加勒比社区，由于加勒比地区共有植物区系而选择了植物。将分析这些物种对致病细菌、阴道加德纳菌以及还将比较阴道中发现的主要有益乳酸菌物种。使用 LC-MS 分析不同组织制剂中生物活性植物化学成分的变化 qToF 和随后的多元统计分析将与海地社区进行民族植物学调查。用于进一步确定加勒比妇女用于治疗妇科疾病的其他植物物种用于继续进行实验室研究。这些数据将能够与其他加勒比地区进行跨文化比较。纽约市社区，还将评估 COVID-19 大流行对纽约市海地妇女的影响他们使用药用植物来促进女性健康从阴道中提取的天然抗菌产品。完整的微生物群落可以为妇科疾病的持续治疗提供低成本和文化上适当的解决方案状况。