Ethnopharmacology of plants used by Haitians in New York City forwomen’s health

纽约市海地人使用的植物对女性健康的民族药理学

• 批准号: 10388470
• 负责人: Ella Vardeman
• 金额: $ 4.26万
• 依托单位: CUNY GRADUATE SCH AND UNIV CTR
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-01 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10388470
• 关键词: Address; Anti-Bacterial Agents; Antimicrobial Effect; Back; Bacteria; Bixa; Botanica; Botanicals; COVID-19 pandemic; Caribbean region; Chemicals; Cities; Coculture Techniques; Collaborations; Commerce; Communities; Country; Cross-Cultural Comparison; Data; Deposition; Disease; Effectiveness; Environment; Ethnobotany; Ethnopharmacology; Evaluation; Feedback; Fractionation; Future; Gardenal; Gardnerella vaginalis; Growth; Haitian; Health; Home; Immigrant; In Vitro; Infection; International; Interview; Investigation; Jatropha; Knowledge; Laboratories; Laboratory Study; Lactobacillus; Literature; Measures; Medical; Medical Students; Medicinal Plants; Methodology; Methods; Monograph; Natural Products; New York; New York City; Pathogenicity; Perception; Persons; Phytochemical; Plant Extracts; Plant Leaves; Plant Preparations; Plant Roots; Plants; Population; Preparation; Prickly Poppy; Property; Public Health; Reporting; Research; Seeds; Self Medication; Sexually Transmitted Diseases; Specimen; Statistical Data Interpretation; Surveys; Taxonomy; Testing; Tissues; Traditional Medicine; United Nations; Vagina; Validation; Variant; Woman; Women' s Health; World Health Organization; antimicrobial; chemical fingerprinting; cost; cultural competence; ethnomedicine; healing; improved; insight; member; metabolomics; microbiota; outreach; pathogenic bacteria; pathogenic microbe; programs; vaginal infection; vaginal microbiota; voucher

Proposal Abstract Approximately 55% of the world’s population lives in cities, and the United Nations estimates that this number will rise to 68% by 2050. Urban ethnobotany, the study of people’s traditional knowledge and use of plants in cities, is increasingly important as people adapt their cultural traditions to changing environments. In New York City (NYC), there have been several urban ethnobotany investigations with Caribbean immigrant groups. However, there has yet to be an ethnobotanical survey that documents and botanically authenticates the medicinal plants used in the Haitian community. Previous research in collaboration with women from the Caribbean in NYC has identified plant species sold in Caribbean shops that have documented use for the treatment of vaginal infections, including sexually transmitted infections and intravaginal cleansing, grouped here as gynecological conditions. Several popular Caribbean plants, such as Jatropha curcas, Argemone mexicana, and Bixa orellana, have recorded medicinal uses in Haitian ethnomedicine as well. These species, while well- studied for their antibacterial properties, have not yet been analyzed for their effects on pathogenic bacteria in the vagina or the protective vaginal microbiota. Ideally, plants used to treat these conditions would have bioactivity against harmful bacteria with minimal disruption to the beneficial vaginal microflora. Natural product research that considers interactions with pathogenic and beneficial bacteria, as well as the compounds responsible, is on the forefront of addressing efficacy of medicinal plants for women’s health. I hypothesize that traditional preparations of the identified species will vary in inhibition of the growth of pathogenic (Gardnerella vaginalis) and beneficial (Lactobacillus) vaginal bacterial due to differences in specialized metabolites. Furthermore, I hypothesize that there will be similarities in plants and women’s health practices between the Haitian and other Caribbean communities in NYC, due to the shared flora in the Caribbean. The selected plant species will be analyzed for their effects on disease-causing bacteria, Gardnerella vaginalis as well as on prominent beneficial Lactobacillus species found in the vagina. Plant extracts will also be compared to determine variation in the composition of bioactive phytochemicals across preparations of different tissues, using LC-MS- qToF and subsequent multivariate statistical analysis. An ethnobotanical survey with the Haitian community will be used to further identify additional plant species used to treat gynecological conditions by Caribbean women for continued laboratory studies. This data will enable cross-cultural comparisons with other Caribbean communities in NYC, and will also assess how the COVID-19 pandemic has influenced NYC Haitian women in their use of medicinal plants for women’s health. Antibacterial natural products from plants that leave the vaginal microflora intact may provide low-cost and culturally-appropriate solutions for ongoing treatment of gynecological conditions.

提案摘要 世界上约有55％的人口居住在城市，联合国估计这一数字 到2050年，将上升到68％。城市民族植物体，研究人们在 城市越来越重要，因为人们将自己的文化传统适应不断变化的环境。在纽约 纽约市（NYC），与加勒比移民团体进行了几项城市民族植物体投资。 但是，尚未进行一项民族植物学调查，以证明并在植物学上验证了该调查 海地社区中使用的药用植物。以前的研究与女性合作 纽约市的加勒比海已确定在加勒比海商店出售的植物物种，这些物种记录了用于 在这里分组的阴道感染的治疗，包括性传播感染和阴道静脉清洁 作为妇科条件。几种受欢迎的加勒比海植物，例如墨西哥人阿格蒙·库尔卡斯（Jatropha Curcas）， 和Bixa Orellana，还记录了海地民族医学的医学用途。这些物种，虽然 研究其抗菌特性的研究尚未分析其对致病细菌的影响 阴道或受保护的阴道菌群。理想情况下，用于治疗这些疾病的植物将具有 对有害细菌的生物活性，对有益的阴道菌群的破坏最小。天然产品 研究与致病性和有益细菌相互作用的研究以及化合物 负责，是解决药物植物对妇女健康的效率的最前沿。我假设这一点 鉴定物种的传统制剂会因抑制致病性而有所不同（Gardnerella） 由于专门代谢产物的差异，阴道）和有益（乳杆菌）阴道细菌。 此外，我假设植物和妇女的健康实践将有相似之处 由于加勒比海地区共享的植物群，海地和其他加勒比社区。选定的植物 将分析物种对引起疾病的细菌的影响，阴道加德纳氏菌以及 在阴道中发现了突出的有益乳酸菌。还将比较植物提取物以确定 使用LC-MS- QTOF和随后的多元统计分析。海地社区的民族植物学调查将 用于进一步识别用于治疗加勒比妇女治疗妇科疾病的其他植物物种 进行持续的实验室研究。这些数据将与其他加勒比人进行跨文化比较 纽约市的社区，还将评估共同19-19大流行如何影响纽约海地妇女 他们将药用植物用于妇女健康。离开阴道的植物的抗菌天然产品 菌群完好 状况。