Northwestern University Skin Biology and Diseases Resource-based Center

西北大学皮肤生物学与疾病资源中心

• 批准号: 10455746
• 负责人: Amy S Paller
• 金额: $ 74.7万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-20 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10455746
• 关键词: 3-Dimensional; Address; Age; Area; Award; Awareness; Back; Biology; Cells; Clinical; Clinical Research; Clinical Trials; Cloning; Clustered Regularly Interspaced Short Palindromic Repeats; Coculture Techniques; Collaborations; Color; Communication; Communities; Complementary DNA; Controlled Environment; Custom; Cutaneous; Databases; Dermatology; Disease; Education; Educational workshop; Electroporation; Environmental Impact; Epidermis; Ethnic group; Experimental Designs; Faculty; Female; Fibroblasts; Fostering; Funding; Gene Silencing; Genes; Genetic Materials; Genetically Engineered Mouse; Goals; Grant; Guide RNA; Healthcare; High Density Lipoproteins; Histology; Human; Hypersensitivity skin testing; Image; Immune; Immune system; Immunofluorescence Immunologic; Immunohistochemistry; Immunologic Monitoring; Immunology; Immunology procedure; In Vitro; Individual; Institution; Instruction; Investigation; Knowledge; Laboratories; Libraries; Mediating; Medicine; Minority; Mission; Morphology; Mus; Nanotechnology; Nerve; Occupational activity of managing finances; Patient Care; Patients; Pattern; Pharmaceutical Preparations; Positioning Attribute; Protocols documentation; RNA Interference; Reporter; Research; Research Personnel; Resources; Scientist; Series; Services; Site; Skin; Skin Tissue; Source; Spherical Nucleic Acids; Structure; Techniques; Technology; Testing; Tissue Engineering; Tissues; Training; Transgenes; Translating; Translational Research; Universities; Viral; Virus; base; bench to bedside; cell bank; cell immortalization; clinical application; cost effective; design; design and construction; environmental agent; expression vector; genome editing; human disease; improved; in vivo; induced pluripotent stem cell; innovation; instrumentation; keratinocyte; male; meetings; melanocyte; member; mouse model; nanoparticle; novel; overexpression; programs; promoter; racial and ethnic; repository; sex; skin color; skin disorder; skin lesion; small hairpin RNA; stem; symposium; translational study; vector

Northwestern University (NU) is recognized for its strong interdisciplinary cutaneous biology research program with particular strength in investigations related to epidermal structure and function. Leveraging this strength, the theme of the NU Skin Biology and Disease Resource Center (SBDRC) is the “Keratinocyte and its Microenviroment”, with its goal to promote outstanding translational research. The NU SBDRC, with its 41 Senior Bench members, 11 Junior Bench members and 12 Collaborating Clinical Associate members, encompasses 13 University departments and 5 divisions within the Department of Medicine. Information generated through the SBDRC will ultimately improve patient care. To accomplish this goal, the SBDRC has the following Cores: (i) Administrative (Admin); (ii) Skin Tissue Engineering and Morphology (STEM); (iii) Translational and Experimental Skin Testing and Immune Tracing (TEST IT); and (iv) Gene Editing, Transduction and Nanotechnology (GET iN). The Admin Core encourages collaboration among the SBDRC Senior and Junior Bench Research members, as well as with the Collaborating Clinical Associate members through its Enrichment Program. Within the Enrichment Program, the SBDRC will support its Pilot and Feasibility (P&F) Program, which will extend cutaneous research in new directions and be a source of new SBDRC members from the NU community. The Admin Core will feature its Minority and Sex Awareness (MSA) Program, which promotes research to elucidate the differences in skin based on sex and skin color. The STEM Core will provide: (i) normal keratinocytes, fibroblasts, and melanocytes, cells from patient lesional skin, and induced pluripotent stem (iPS) cells, including an extensive cell bank; (ii) 3D organotypic cultures of human and mouse epidermis; (iii) keratinocyte co-cultures, including with immune cells and nerves; and (iv) an extensive array of morphogenetic processing services. The TEST IT Core focuses on the important relationship between keratinocytes and the skin immune system, and provides instrumentation and immunological assay services, as well as the unique opportunity for human testing in a controlled environment. The GET iN Core will generate: (i) lentiviral and retroviral constructs to overexpress or silence genes and to express reporters in skin cells; (ii) viruses for generating and differentiating iPSCs; (iii) CRISPR/Cas constructs for introduction into keratinocytes; (iv) nonviral approaches for delivering CAS9 and guide RNAs; and (v) a variety of other nonviral delivery techniques using nanotechnology (spherical nucleic acids/SNAs, high density lipoprotein/HDL-like nanoparticles) and Nanofountain Probe-based Electroporation. STEM, TEST IT and GET iN cores interdigitate with each other and other university cores, provide education through training and interpretation of results, and have strong translational components. The NU SBDRC is ideally positioned to provide users with opportunities to take their basic findings into a clinical setting, which will enable NU skin biologists to make important strides in understanding and treating diseases of the skin.

西北大学（NU）的认可是强大的互联网皮肤生物学研究计划 在与表皮结构和功能有关的研究中具有特殊的强度。 NU皮肤生物学和疾病资源中心（SBDRC）的主题是“角质形成细胞及其 Microenviroment”，其目标是促进出色的翻译研究。NUSBDRC，其41 高级替补席成员，11名初级席成员和12名合作临床副成员， 包括医学部门的13个大学部门和5个部门 通过SBDRC产生的顾客将改善patiesent护理。 以下核心：（i）行政管理（ii）皮肤组织工程和形态； 翻译和实验性皮肤测试和免疫追踪（测试）和（iv）基因编辑 转导和纳米技术（进入）。 高级和初级替补席研究成员以及合作临床协会 通过其丰富计划。 Feast (P & F) Program, Which WILL EXTEND Cutaneous Research in New Directions and be a Source of New New New New New New New New New New New New New New New New New New New New New New New New New New New New New新的新。 NU社区的SBDRC成员。 程序，促进了根据性别和皮肤颜色阐明皮肤差异的研究 核心将提供：（i）正常的角质形成细胞，成纤维细胞和黑素细胞，来自贴皮病变皮肤的细胞 软茎（IPS）细胞，包括广泛的细胞库； 和小鼠表皮； 大量的形态发生处理服务。 角质形成细胞与皮肤免疫系统之间的关系，并提供仪器和 免疫学分析服务是在受控设想中进行人类测试的独特机会。 核心将产生：（i）慢病毒和逆转录病毒构建体过表达或沉默基因以及 在皮肤细胞中表达记者； 引入角质形成细胞； 使用纳米技术（球形核酸/SNA，高密度）的其他各种非病毒输送技术 脂蛋白/HDL样纳米颗粒）和基于纳米的探针电穿孔。 在核心方面相互互动和其他大学核心，通过培训和 结果的解释，并具有静态成分。 为用户提供基本发现的机会进入临床环境，这将使NU皮肤能够 生物学家在理解和治疗皮肤疾病方面取得了重要成就。