Physiology Core

生理学核心

基本信息

批准号：
10711694
负责人：
CLIFFORD JAMES ROSEN
金额：
$ 31.05万
依托单位：
MAINEHEALTH
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-09-01 至 2028-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10711694
关键词：
3-Dimensional Address Age Animal Model Animals Applications Grants Award Behavior Biochemical Pathway Bioenergetics Body Composition Body Temperature Body Weight Cell physiology Cells Cellular Metabolic Process Centers of Research Excellence Communication Consultations Controlled Environment Cost Analysis Counseling Coupled Data Data Analyses Data Set Digital X-Ray Dual-Energy X-Ray Absorptiometry Education Energy Metabolism Equipment Exercise Experimental Designs Faculty Fee-for-Service Plans Food Funding Future Generations Genus Hippocampus Glycolysis Goals Grant Heart Rate Human Resources Image In Vitro Incubators Institution Leadership Martes zibellina Measures Mesenchymal Metabolic Mitochondria Modeling Mus Nature New England Output Phase Physical activity Physiological Physiology Pilot Projects Positioning Attribute Postdoctoral Fellow Productivity Publications Quality Control Reader Reporting Research Research Design Research Institute Research Personnel Resources Respiration Roentgen Rays Services Site Spirometry Structure Suggestion System Technology Telemetry Temperature Time Tissues Training Universities Water consumption anticancer research behavioral phenotyping behavioral study clinical investigation cold temperature cytate experimental study flexibility food consumption gas analyzer graduate student human disease human model imager in vivo insight medical schools meetings metabolic abnormality assessment metabolic phenotype mouse model mutant neuroregulation new technology novel oxidation programs research study response service utilization sex success tissue culture training opportunity treadmill undergraduate student

项目摘要

Physiology Core (C. Rosen, Core Director) The long-term goal of the Physiology Core is to provide high quality in vivo and in vitro metabolic phenotyping services for COBRE projects, other MaineHealth researchers, and outside investigators. Phase I accomplishments include expanding and upgrading our metabolic phenotyping through the acquisition of 8 G2 HR E-Mitter telemetry systems, a Seahorse XF96 analyzer, a Cytation I cell imager, a Faxitron UltraFocus DXA X-ray machine and a Sable Systems respirometry treadmill, based on our users’ needs. We successfully supported our COBRE investigators and non-COBRE institutional faculty, research staff, and trainees, thereby meeting our targeted metrics for support of funded grant applications and publications. Over the last five years, all four original project leads received independent R01 funding, and were frequent users of the Physiology Core, for experimental services or consultation with Dr. Rosen. Their studies included analysis of cellular bioenergetics, body composition, and metabolic and behavioral studies. Moreover, we have successfully increased outside utilization through collaborative projects with the University of New England, Solarea Bio (Cambridge, MA), Mt. Sinai School of Medicine, Tufts and Cornell Universities. These resulted in steady increases in annual service fee revenue that is integral for sustainability. Use of the Physiology Core was instrumental in the funding of a multi-PI U19 from NIA to Drs. Zaidi (Mt. Sinai) and Rosen (Physiology Core Director). Publications in PNAS, J Biol Chem, J Clinical Investigation and JCI Insight, eLife, Cancer Research, Cell Metabolism, Nature Communications, and Cell Reports were direct results of experiments from this Core. Importantly, the Physiology Core was supportive of young investigators in training, including the successful funding of an F31 and an F32. Most of the pilot project awardees used one of the features of this Core, and educational programs related to new technical aspects for Core equipment were provided to undergraduate and graduate students as well as postdoctoral fellows. Phase II offers new opportunities for the Physiology Core in respect to novel technologies, expanded use of current equipment and greater training opportunities. In addition, we were recently awarded a COBRE equipment supplement to expand the number of metabolic cages and integrate a temperature-controlled incubator within the system. Thus in Phase II, we have expanded our equipment and capacity to meet the high demand for metabolic and behavioral phenotyping, and will also provide new services for in vivo studies at thermoneutrality and at cold temperatures. We will maintain our highly utilized services for advanced cellular and mitochondrial bioenergetic analysis, in addition to providing expert assistance in research study design and implementation and data analysis. The successful leadership, management, and operational principles of this Core will remain in place, and scientifically support the project leads Drs. Lynes, Li, and Agarwal, and other MaineHealth and external investigators.

生理学核心（C. Rosen，核心主任）生理学核心的长期目标是提供高质量的体内和体外代谢表型分析为 COBRE 项目、其他 MaineHealth 研究人员和外部 I 期研究人员提供服务。成就包括通过收购 8 G2 扩展和升级我们的代谢表型 HR E-Mitter 遥测系统、Seahorse XF96 分析仪、Cytation I 细胞成像仪、Faxitron UltraFocus 根据用户的需求，我们成功地使用了 DXA X 射线机和 Sable Systems 呼吸测定跑步机。支持我们的 COBRE 研究人员和非 COBRE 机构教职人员、研究人员和实习生，从而满足我们在过去五年中资助资助申请和出版物的目标指标。所有四个原始项目负责人都获得了独立的 R01 资助，并且是 Physiology 的频繁用户核心，用于实验服务或咨询罗森博士他们的研究包括细胞分析。此外，我们还成功地进行了生物能量学、身体成分以及代谢和行为研究。通过与新英格兰大学 Solarea Bio 的合作项目增加外部利用率（马萨诸塞州剑桥）、西奈山医学院、塔夫茨大学和康奈尔大学这些大学的成绩稳定。年度服务费收入的增加是可持续发展不可或缺的一部分。在 NIA 向 Zaidi（西奈山）和 Rosen（生理学核心）博士资助的多 PI U19 中发挥了重要作用主任）发表于《PNAS》、《J Biol Chem》、《J Clinical Investigation》和《JCI Insight》、《eLife》、《癌症研究》、《细胞代谢》、《自然通讯》和《细胞报告》是该核心实验的直接结果。重要的是，生理学核心支持年轻研究者的培训，包括成功的 F31 和 F32 的资助大多数试点项目获奖者都使用了该核心的功能之一，以及向本科生提供了与核心设备新技术相关的教育计划第二阶段为生理学提供了新的机会。核心在于新技术、扩大现有设备的使用和更多的培训机会。此外，我们最近获得了 COBRE 设备补充，以扩大代谢数量因此，在第二阶段，我们进行了扩展。我们的设备和能力可以满足代谢和行为表型分析的高需求，并且还将为热中性和低温下的体内研究提供新服务我们将维持我们的服务。高度利用高级细胞和线粒体生物能分析服务，此外还提供研究设计和实施以及数据分析方面的专家协助，该核心的管理和运作原则将保持不变，并科学地支持该项目 Lynes、Li 和 Agarwal 博士以及其他缅因州卫生局和外部研究人员领导。