African-American Susceptibility to Periodontal Disease due to Electronic Nicotine Delivery Systems (ENDS) Usage

非裔美国人因使用电子尼古丁传送系统 (ENDS) 而易患牙周病

• 批准号: 10453478
• 负责人: Jonathan Henry Shannahan
• 金额: $ 8.35万
• 依托单位: GEORGIA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-03 至 2022-02-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10453478
• 关键词: Address; Aerosols; Affective; African American; Air; Antioxidants; Behavior; Behavioral; Biological; Biological Markers; Biological Models; Caucasians; Cell model; Cells; Characteristics; Cigarette; Clinical assessments; Cognitive; Communities; Coupled; DNA Damage; DNA Repair; Data; Dental; Dental Hygienists; Development; Devices; Discrimination; Disease; Disease Outcome; Disease susceptibility; Early identification; Ecological momentary assessment; Electronic Nicotine Delivery Systems; Epithelial; Event; Generations; Gingiva; Hazardous Chemicals; Health; Health Status; Human; Immune response; In Vitro; Individual; Inflammation; Injury; Interest Group; JUUL; Knowledge; Life; Link; Liquid substance; Manufacturer Name; Mediating; Mesenchymal; Methods; Molecular; Molecular Epidemiology; Moods; Mouth Diseases; Oral; Oral Examination; Oral health; Outcome; Oxidative Stress; Oxidative Stress Induction; Participant; Pathway interactions; Pattern; Periodontal Diseases; Phenotype; Population; Predisposition; Prevalence; Prevention strategy; Proteomics; Protocols documentation; Psyche structure; Psychosocial Factor; Questionnaires; Race; Risk; Risk Factors; Role; Saliva; Severities; Smoke; Smoking Behavior; Socioeconomic Status; Stress; Swab; Testing; Therapeutic; Time; Tobacco; Toxic effect; Toxicology; Variant; base; biomarker panel; caucasian American; cigarette smoking; clinical application; cohort; comparative; craniofacial; design; disorder prevention; disorder risk; early onset; experience; health disparity; high risk; innovation; insight; keratinocyte; metabolomics; novel; novel marker; oxidative DNA damage; physical conditioning; preference; prevent; psychosocial stressors; racial difference; racial discrimination; real time monitoring; recruit; response; saliva sample; sex; specific biomarkers; tobacco products; tobacco user

Project Summary The acceptance and use of electronic nicotine delivery systems (ENDS) within the African-American (AA) community may widen tobacco-related health disparities such as gingival inflammation (GI), the precursor to periodontal disease. However, how and why ENDS use may pose GI risk is unclear as there is a lack of comprehensive data on smoking behaviors, exposure, and disease outcomes in AA ENDS users. We hypothesize that AA ENDS users may be more susceptible to ENDS-mediated oral health outcomes due to variations in ENDS preference, usage, and biological responses. To address this hypothesis, we will establish a behavior-exposure-toxicity-disease paradigm utilizing aims specifically designed to evaluate behaviors (Aim 1), common ENDS products (Aim 2), and susceptibility (Aim 3). This innovative approach will facilitate the identification of risk factors and biomarkers that can be applied to disease prevention strategies. In Aim 1, we will recruit cohorts of current AA and Caucasian ENDS users and never-tobacco users to evaluate patterns of ENDS usage behaviors, oral health, and risk factors. In this aim, we will employ questionnaires and mobile ecological momentary assessments coupled with real-time monitoring to examine ENDS puff topography within each group. By using this mixed methods approach, we will limit recall bias and also capture moment to moment phenomena that may occur differentially in these two ENDS user groups. To elucidate the role of specific group and product-based ENDS exposure scenarios we will utilize an in vitro air liquid interface cellular model system in Aim 2. Using specific group exposure scenarios (identified by puff topography characterization in Aim 1) and established protocols for comparative toxicity assessments we will identify differential biomarkers associated with ENDS aerosol exposures. Specifically, this will include comparisons of cellular responses induced by differing ENDS generation products and brands as well as usage patterns. In Aim 3, we will utilize a molecular epidemiology approach to assess the impact of differential exposures in our AA and Caucasian ENDS cohorts by using participant saliva and gingival cells to identify biomarkers of oxidative stress, DNA damage, and other indicators commonly found in GI. Specifically, we aim to establish a pathway between ENDS preference, puff topography, and toxicological pathways contributing GI development that may be exacerbated in the AA population. Our project outcomes will be a biomarker panel that may provide critical insight into ENDS-mediated GI susceptibility in AA.

项目摘要 非裔美国人（AA）中电子尼古丁输送系统（末端）的接受和使用 社区可能会扩大与烟草相关的健康差异，例如牙龈炎症（GI），这是 牙周疾病。但是，由于缺乏 AA中吸烟行为，暴露和疾病结果的全面数据结束了用户。我们 假设AA结束用户可能更容易受到末端介导的口腔健康结果的影响 目的偏好，用法和生物学反应的变化。为了解决这一假设，我们将建立一个 专门设计的目的是评估行为（AIM 1）， 普通端产品（AIM 2）和敏感性（AIM 3）。这种创新的方法将促进 识别可应用于疾病预防策略的危险因素和生物标志物。 在AIM 1中，我们将招募当前的AA和高加索人的群体，并结束用户和永不烟的用户 评估用法行为，口腔健康和危险因素的最终模式。在这个目标中，我们将雇用 问卷和移动生态瞬时评估以及实时监控以检查 每组中的末日粉扑地图。通过使用这种混合方法方法，我们将限制召回偏见和 还捕获这两个末端中可能发生差异化的时刻现象。到 阐明特定组和基于产品的末端暴露情况的作用，我们将使用体外空气 AIM 2中的液体界面蜂窝模型系统。使用特定的组曝光场景（由Puff确定 AIM 1）和对比较毒性评估的既定协议中的地形表征我们将 确定与末端气溶胶暴露相关的差异生物标志物。具体来说，这将包括 由不同的末端生成产品和品牌以及用法引起的细胞反应的比较 模式。在AIM 3中，我们将利用一种分子流行病学方法来评估差异的影响 通过使用参与者唾液和牙龈细胞来识别我们的AA和高加索人终点的暴露 GI中常见的氧化应激，DNA损伤和其他指标的生物标志物。具体来说，我们的目标 在末端偏好，泡芙地形和毒理学途径之间建立途径 在AA人群中可能会加剧的发展。我们的项目成果将是一个生物标记面板 可能会对AA中末端介导的GI敏感性提供批判性洞察力。