Pets and the Infant Microbiome: Effect on Immune Maturation & Atopic Asthma

宠物和婴儿微生物组：对免疫成熟的影响

基本信息

批准号：
8507139
负责人：
Christine C Johnson
金额：
$ 175.36万
依托单位：
HENRY FORD HEALTH SYSTEM
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-07-06 至 2017-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8507139
关键词：
2 year old 6 year old 9 year old Address Affect Age Age-Months Allergic Allergic Disease Animals Antibody Formation Asthma Birth Canis familiaris Child Childhood Asthma Cohort Studies Communities Development Disease Dust Environment Exposure to Extrinsic asthma Feces Felis catus Fostering Gastrointestinal tract structure Goals Home environment House Dust Household Hypersensitivity IgE Immune Immune response Immune system Infant Instruction Intestines Life Lung Lung Inflammation Measures Medical Methods Morbidity - disease rate Newborn Infant Pattern Prevalence Primary Prevention Program Research Project Grants Recruitment Activity Research Risk Sampling Services United States atopy cohort cost disorder prevention early life exposure environmental change environmental intervention gut microbiota immune function infancy innovation microbial microbiome mouse model pet animal protective effect

项目摘要

This revised Program Project Grant (PPG) application seeks an increased understanding of the relationships between dog or cat exposure during infancy and a lower risk of allergic asthma. We believe that this protective association is related to different patterns of microbial stimulation during immune development. Four synergistic Projects will examine our hypothesis that the presence of pets in a home results in a more diverse bacterial community composition (BCC) of the dust in the home which in turn influences the development of the gut BCC of a newborn infant living in the home. A more diverse gut BCC shifts the maturation of the infant's immune system such that later immune responses are less likely to produce IgE antibody responses and allergic asthma. Project 1 examines the relationships between the presence of a dog or cat in a home, the BCC of dust in the home, the BCC of stools of infants living in homes and allergic sensitization at 2 years of age. An innovative, culture-independent microarray (G3 PhyloChip) will be used to characterize the BCCs utilizing previously collected samples from the ongoing WHEALS birth cohort study. Changes in home BCC following introduction of a dog are also examined. To further examine relationships between dog exposure and immune development. Project 2 will recruit a new birth cohort of children either living with or without a dog, measure infant stool BCC, and follow the cohort with detailed studies of immune function until 18 months of age to determine the impact of dog exposure on immune maturation. Project 3 uses mouse models of allergic asthma to further examine the influence of house dust from homes with and without dogs on immune responses that result in lung inflammation. The use of mouse models allows more detailed studies of immune functions from multiple compartments of the body than are possible with human children. Project 4 again utilizes the existing WHEALS birth cohort to examine the relationships between dog or cat exposure during the first year of age, 6-month infant stool BCC, and the presence of allergic asthma at 9 years of age. The Projects are supported by five Cores which each provide essential services to all four Projects.

这项修订后的计划项目拨款 (PPG) 申请旨在加深对婴儿期狗或猫接触与降低过敏性哮喘风险之间关系的了解。我们认为这种保护性关联与免疫发育过程中微生物刺激的不同模式有关。四个协同项目将检验我们的假设，即家中宠物的存在会导致家中灰尘的细菌群落组成 (BCC) 更加多样化，进而影响家中新生儿肠道 BCC 的发育。更加多样化的肠道 BCC 会改变婴儿免疫系统的成熟度，从而使后续的免疫反应不太可能产生 IgE 抗体反应和过敏性哮喘。项目 1 检查家中是否有狗或猫、家中灰尘的 BCC、家中婴儿粪便的 BCC 与过敏之间的关系 2岁开始过敏。一种创新的、独立于培养物的微阵列（G3 PhyloChip）将用于利用之前从正在进行的 WHEALS 出生队列研究中收集的样本来表征 BCC。还检查了引入狗后家庭 BCC 的变化。进一步研究狗的暴露与免疫发育之间的关系。项目 2 将招募一个新出生的儿童队列，其中有或没有养狗，测量婴儿粪便 BCC，并跟踪该队列，对免疫功能进行详细研究，直至 18 个月大，以确定接触狗对免疫成熟的影响。项目 3 使用过敏性哮喘小鼠模型来进一步研究养狗和不养狗的家庭中的灰尘对导致肺部炎症的免疫反应的影响。与人类儿童相比，使用小鼠模型可以对身体多个部位的免疫功能进行更详细的研究。项目 4 再次利用现有的 WHEALS 出生队列来检查一岁时接触狗或猫、6 个月婴儿粪便 BCC 和 1 岁时过敏性哮喘的存在之间的关系。 9岁。这些项目由五个核心支持，每个核心为所有四个项目提供基本服务。