Understanding mechanisms of sex specification and phenotypes in an animal model

了解动物模型中性别规范和表型的机制

• 批准号: 10397631
• 负责人: GARY J ROSE
• 金额: $ 7.63万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-05-01 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10397631
• 关键词: Address; Adult; Amygdaloid structure; Animal Model; Anterior; Area; Arginine; Behavior; Behavioral; Behavioral Mechanisms; Biological; Biological Process; Brain; Brain region; Cell Nucleus; Color; Competence; Courtship; Electrophysiology (science); Environment; Etiology; Excision; FOS gene; Female; Fishes; Gender Identity; Gender identity disorder; Gene Expression; Glutamates; Goals; Human; Hypothalamic structure; Immediate-Early Genes; Immunohistochemistry; Iontophoresis; Label; Lateral; Mediating; Midbrain structure; Modeling; Monitor; Neurobiology; Neurons; Organism; Phase; Phenotype; Phenotypic Sex; Physiological; Play; Positioning Attribute; Preoptic Areas; Process; Product Labeling; Property; Proxy; Public Health; Regulation; Research; Role; Social Behavior; Social Dominance; Social Environment; Social status; System; Techniques; Telencephalon; Testing; Time; Up-Regulation; Vasotocin; Work; base; behavioral plasticity; court; diencephalon; experimental study; gender dysphoria; in vivo; male; microstimulation; midbrain central gray substance; neural circuit; neurophysiology; novel; programs; receptor; relating to nervous system; response; sex; skin color; social; social structure

Mechanisms of behavioral sex plasticity in sex-changing wrasses The biological processes that underlie behavioral sex phenotype are incompletely understood. Organisms that naturally undergo sex transformations in response to changes in their social environment provide excellent systems in which to investigate mechanisms of behavioral sex specification. Bluehead wrasses in particular are well suited for addressing this problem. Adult females can switch sex in response to changes in social structure. Further, behavioral sex transformation can occur rapidly, thereby providing an opportunity to investigate the neurobiological basis of this plasticity. We propose to further develop this system as a model for investigating mechanisms of plasticity of behavioral sex phenotype. The proposed work builds on our ‘priming/gating’ hypothesis of behavioral sex transformation; this hypothesis accounts for the relation between social rank and competency for transformation. Our first objective is to compare the expression of the immediate-early genes, c-Fos and p-S6, in courting and non- courting fish. We expect that results will further elucidate the neural circuits that control the expression of male-typical courtship behavior. To test for causal roles of these circuits in courtship behavior, we plan to iontophorese glutamate to discretely activate particular brain regions and monitor courtship-related changes in skin coloration. Our goal is to identify properties of neural circuits that underlie competency to undergo behavioral sex transformation.

行为性可塑性在性别改变包裹中的机制 行为性表型基础的生物学过程尚不完全理解。 自然会因社会变化而自然会发生性别转变的生物 环境提供了调查行为性机制的出色系统 规格。尤其是蓝头套包装非常适合解决此问题。成人 女性可以响应社会结构的变化而改变性。此外，行为性行为 转型可以迅速发生，从而提供了调查的机会 这种可塑性的神经生物学基础。我们建议进一步开发该系统作为模型 研究行为性表型可塑性的机制。拟议的工作建立 在我们的行为性转化的“启动/门控”假设上；该假设是 社会等级与转型能力之间的关系。我们的第一个目标是 比较求求和非 - 求鱼。我们预计结果将进一步阐明控制的神经回路 表达男性典型的求爱行为。测试这些电路中的因果关系 求爱行为，我们计划离子谷氨酸谷氨酸，以离散地激活特定的大脑 区域和监控肤色与求爱相关的变化。我们的目标是确定 神经元电路的特性，其能力是经历行为转化的能力。