Core C: Metabolic Phenotyping Core

核心 C：代谢表型核心

• 批准号: 10213807
• 负责人: Justin L Grobe
• 金额: $ 5.5万
• 依托单位: MEDICAL COLLEGE OF WISCONSIN
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-01 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10213807
• 关键词: Adrenalectomy; Animals; Biomedical Research; Biometry; Blood; Blood flow; Body Composition; Brain; Calorimetry; Cannulations; Cell Respiration; Consult; Core Facility; Data Analyses; Diabetes Mellitus; Doctor of Philosophy; Eagles; Equipment; Experimental Designs; Faculty; Genetic; Homeostasis; Iowa; Laboratories; Medicine; Metabolic; Methods; Molecular; Mus; Nuclear Magnetic Resonance; Operative Surgical Procedures; Ovariectomy; Phenotype; Publishing; Pump; Regulation; Reproducibility; Research; Rodent; Services; Signal Transduction; Standardization; Statistical Data Interpretation; Students; Surgeon; System; Systems Analysis; Techniques; Training; Universities; Urinalysis; base; cardiometabolism; college; design; experience; graduate student; implantation; improved; instrumentation; metabolic phenotype; mouse model; phenotypic data; programs; surgical service

Summary / Abstract The objective of the current program is to further our understanding of the molecular mechanisms within the brain that contribute to cardiometabolic control. The current core is designed to provide technical and intellectual support for assessments of energy homeostasis in mouse models employed by all four projects within the program. Through this core, projects will have access to an array of equipment used to assess energy homeostasis including multiplexed systems (Promethion, OxyMax), NMR-based body composition analyzers, bomb calorimeters, metabolic caging systems, respirometers, direct calorimeters, cellular respiration systems, urinalysis/blood analysis systems, etc. In addition, core staff will provide surgical support for all projects, and the PI will provide statistical support and training for all projects. The centralizing of these endpoints into a core will support increased rigor and reproducibility of all metabolic phenotyping data across the program.

摘要/摘要 当前计划的目标是进一步了解我们对分子机制的理解 有助于心脏代谢控制的大脑。当前的核心旨在提供技术和 为所有四个项目所采用的小鼠模型中的能量稳态评估提供智力支持 程序内。通过这个核心，项目将能够使用一系列用于评估的设备 能量稳态，包括多重系统（Promethion、OxyMax）、基于 NMR 的身体成分 分析仪、炸弹热量计、代谢笼系统、呼吸计、直接热量计、细胞 呼吸系统、尿液分析/血液分析系统等。此外，核心人员将提供手术支持 PI将为所有项目提供统计支持和培训。这些的集中化 将终点纳入核心将支持提高所有代谢表型数据的严谨性和可重复性 程序。