Biofunctional sealant with peptides to extend lifespans of Class V restorations

含有肽的生物功能密封剂可延长 V 类修复体的使用寿命

基本信息

批准号：
10371864
负责人：
Nicholas G Fischer
金额：
$ 5.26万
依托单位：
UNIVERSITY OF MINNESOTA
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-03-01 至 2025-02-28
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10371864
关键词：
Address Adhesions Adhesives Affinity Aging Air Amino Acids Amputees Bacteria Bacterial Infections Binding Biocompatible Materials Biological Biology Catheters Cells Chemicals Chemistry Clinical Coculture Techniques Data Dental Dental Implants Dental Schools Dentin Dentistry Devices Disease Doctor of Philosophy Dopa Elderly Elements Engineering Environment Epithelial Attachment Esthetics Extracellular Matrix Extracellular Matrix Proteins Failure Fibroblasts Flow Cytometry Formulation Gingiva Gingival Recession Goals Grant Health Care Costs Hemidesmosomes Implant Individual Integrin alpha6beta4 Integrins Knowledge Lead Learning Longevity Maintenance Mechanics Mediating Methacrylates Minnesota Modeling Modernization Molecular Biology Techniques Nature Oligopeptides Oral Oral cavity Patients Peptides Performance Periodontitis Population Prevalence Quality of life Research Research Personnel Resolution Roentgen Rays Route Scientist Series Signal Transduction Societies Spectrum Analysis Surface System Techniques Technology Testing Tissues Tooth structure Training Universities Up-Regulation Water base biomaterial compatibility career career development combat design experience improved innovation keratinocyte microbial multidisciplinary novel peri-implantitis prevent response restoration restorative dentistry restorative material sealant tooth surface

项目摘要

Class V dental restorations, those on the lower third of the tooth, are the least durable type of dental restoration and are quickly increasing in placement due to a rapidly aging society. Water and bacteria mediated destruction of the dentin/restoration interface is the main cause of failure. Maintenance of integrity between the restorative material and surrounding tissue is an unexplored route to increase lifespans of Class V restorations to shield the vulnerable adhesive interface from bacteria-mediated degradation, promote restoration stability, and increase restoration aesthetics. Tissue attachment to current Class V restorative surfaces does not occur as current restorative materials lack any activity designed to influence tissue responses. This lack of attachment reveals the vulnerable dentin/restoration interface and exacerbates Class V failure. Functional tissues near Class V restorations may be able to naturally prevent subgingival plaque and lead to longer lifespans by exploiting the tissue. Our central goal is to develop new materials and apply experimental approaches toward reducing Class V failure. In Aim 1, we aim to understand oral cellular activation responsible for signaling and test candidate molecules for their ability to survive enzymatic degradation. In Aim 2, we will demonstrate our system enables biocompatible, facile, rapid, and robust delivery of biofunctional molecules to the material surface and retains molecules through a series of challenges meant to simulate the oral environment. The results will potentially extend lifespans of Class V dental restorative materials and reduce longterm healthcare costs. This training grant will provide pivotal opportunities to learn techniques including flow cytometry, high resolution X-ray photoelectron spectroscopy, and molecular biology techniques. Improved understanding of these techniques will further my progress toward becoming an independent academic researcher at a dental school.

V级牙齿修复体是牙齿下三分之一的牙齿修复类型的牙齿恢复类型，由于社会迅速老化，牙齿修复体类型最低，并且正在迅速增加位置。水和细菌介导的牙本质/恢复界面的破坏是失败的主要原因。维持修复材料和周围组织之间的完整性是一条未开发的途径，可以增加V级修复体的寿命，以保护脆弱的粘合剂界面免受细菌介导的降解，促进恢复稳定性并增加恢复美学的降解。由于当前的修复材料缺乏旨在影响组织反应的活动，因此不会发生在当前V级恢复表面上的组织附着。缺乏依恋揭示了脆弱的牙本质/修复界面，并加剧了V类失败。 V级修复物附近的功能组织可能能够自然地防止尺寸牙菌斑，并通过利用组织而导致更长的寿命。我们的核心目标是开发新材料，并采用实验方法来减少V类失败。在AIM 1中，我们旨在了解负责信号传导和测试候选分子的口腔细胞活化，以使其生存酶促降解的能力。在AIM 2中，我们将展示我们的系统可以实现生物相容性，易于，快速且可靠的生物功能分子向材料表面递送，并通过一系列旨在模拟口服环境的挑战来保留分子。结果将有可能延长V级修复材料的寿命并降低长期医疗保健成本。该培训赠款将为学习技术提供关键机会，包括流式细胞术，高分辨率X射线光电子光谱和分子生物学技术。对这些技术的了解得以提高，将进一步成为牙科学校的独立学术研究员的进步。