Light-propelled dental adhesives with enhanced bonding capability

具有增强粘合能力的光驱动牙科粘合剂

• 批准号: 10741660
• 负责人: Devatha P. Nair
• 金额: $ 42.9万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-12 至 2025-07-11
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10741660
• 关键词: Address; Adhesions; Adhesives; Amalgam; Artificial Saliva; Bisphenol A-Glycidyl Methacrylate; Collagen; Collagen Fibril; Color; Complex; Composite Resins; Dental; Dentin; Diffusion; Ensure; Environment; Enzymes; Esthetics; Ethanol; Fatigue; Formulation; Goals; Hybrids; Hydrophobic Interactions; Hydrophobicity; Hydroxyapatites; Infiltration; Kinetics; Light; Link; Longevity; Matrix Metalloproteinases; Mechanics; Methacrylates; Movement; NanoGel; Nanostructures; Oral; Oral cavity; Penetration; Performance; Plant Resins; Play; Polymers; Predisposition; Protocols documentation; Research; Residual state; Role; Sampling; Scheme; Source; Surface; Swelling; Techniques; Tooth structure; Visible Radiation; Water; Wettability; Work; amphiphilicity; azobenzene; bisphenol A; collagen scaffold; collagenase; composite restoration; conditioning; copolymer; crosslink; demineralization; dental adhesive; design; functional group; hydrophilicity; interfacial; mechanical properties; monomer; nanopolymer; novel; pi bond; polymerization; preference; preservation; pressure; prevent; restoration; restorative dentistry; restorative material; tooth surface; Address; Adhesions; Adhesives; Amalgam; Artificial Saliva; Bisphenol A-Glycidyl Methacrylate; Collagen; Collagen Fibril; Color; Complex; Composite Resins; Dental; Dentin; Diffusion; Ensure; Environment; Enzymes; Esthetics; Ethanol; Fatigue; Formulation; Goals; Hybrids; Hydrophobic Interactions; Hydrophobicity; Hydroxyapatites; Infiltration; Kinetics; Light; Link; Longevity; Matrix Metalloproteinases; Mechanics; Methacrylates; Movement; NanoGel; Nanostructures; Oral; Oral cavity; Penetration; Performance; Plant Resins; Play; Polymers; Predisposition; Protocols documentation; Research; Residual state; Role; Sampling; Scheme; Source; Surface; Swelling; Techniques; Tooth structure; Visible Radiation; Water; Wettability; Work; amphiphilicity; azobenzene; bisphenol A; collagen scaffold; collagenase; composite restoration; conditioning; copolymer; crosslink; demineralization; dental adhesive; design; functional group; hydrophilicity; interfacial; mechanical properties; monomer; nanopolymer; novel; pi bond; polymerization; preference; preservation; pressure; prevent; restoration; restorative dentistry; restorative material; tooth surface

Project Summary/Abstract: The growing preference for aesthetically pleasing composite restorations is reflected in the over 122 million dental restorations placed annually in the U.S. However, the replacement of failed restorations accounts for greater than 50% of all restorative dental work. The relatively short duration composite restorations last in comparison to traditional restorations (e.g., amalgam) is attributed to a weak hybrid layer, which is the layer that bonds the adhesive to the tooth structure. Bonding to dentin in the tooth in a consistent and stable manner is challenging. While applying the adhesive resin on etched dentin, relying on simple diffusion to homogenously infiltrate the dentinal tubules up to the etch depth in the presence of pulpal pressure makes adhering to dentin less predictable and more technique sensitive. Leaving exposed collagen within the hybrid layer compromises its mechanical strength and long-term stability by leaving it vulnerable to proteolytic degradation. We propose a solution to this problem by introducing light-responsive, methacrylated nanoadditives that swell and disperse within conventional adhesives and are designed to move away from a visible light source. By using light-induced resin mobility, we propose to drive the controlled, light-activated, homogenous diffusion of hydrophobic and hydrophilic adhesive resin components within dentinal tubules up to the depth of etching. This will ensure that the demineralized surfaces and exposed collagen networks are now evenly and homogenously enveloped by resin within the hybrid layer to form a mechanically robust anchor with the tooth. Subsequently, pendant functionality on the surface of the nanoadditives will further enhance the strength of the resin-dentin interface and the stability of the collagen by introducing crosslinking via primary and secondary bonds. The presence of labile functional groups that can induce intermolecular and intermicrofibrillar collagen crosslinks, along with hydrophobic interactions can enhance the mechanical properties and biostability of the adhesive layer. Increased crosslinking will also decrease the susceptibility of the hybrid layer to bacterial collagenase and MMPs. Therefore, we will specifically aim to 1) synthesize 4 to 6 light-responsive nanoadditives that can be incorporated within conventional adhesive resins and study adhesive resin propulsion as a function of 430-480 nm light exposure, polymerization kinetics, degree of conversion, and mechanical properties under simulated pulpal pressure. Toward this end, light-responsive nanoadditives will be synthesized using a solution polymerization protocol and the adhesive resin-nanoadditive networks will be characterized based on the type and concentration of light-responsive moieties within the networks. Pendant functionality introduced via nanoadditives to enhance bioadhesion and collagen crosslinking will also be evaluated. In Aim 2, we will establish the conditions by which the nanoadditives can enhance bioadhesion within the hybrid layer via additional crosslinking and characterize the µTBS, interfacial gap formation, and stability of the resin-tooth interface in the oral physiochemical environment.

项目摘要/摘要： 超过1.22亿牙齿反映了对美学上令人愉悦的复合修复体的偏爱越来越多 但是，每年在美国进行的修复体，替换失败的修复体占了超过50％ 所有恢复性牙科工作。与传统修复体相比，相对短持续时间复合修复体最后一次 （例如，汞合金）归因于弱杂种层，该层是将粘合剂与牙齿结构粘合的层。结合 挑战以一致且稳定的方式在牙齿中牙齿。在将粘合剂树脂涂在蚀刻牙本上时， 依靠简单的扩散以同质性渗透到牙髓情况下牙齿深度至蚀刻深度 压力使粘附在牙本质上降低了可预测，更敏感的技术。将暴露的胶原蛋白留在杂种中 图层通过使其容易受到蛋白水解降解的影响来损害其机械强度和长期稳定性。我们 通过引入膨胀和分散的光响应，甲基化的纳米additives，提出解决此问题的解决方案 在传统的粘合剂中，旨在远离可见光源。通过使用光诱导的树脂 机动性，我们建议驱动疏水和亲水的受控，光激活，同质扩散 牙齿小管中的粘合剂树脂成分直至蚀刻深度。这将确保债务 现在的表面和暴露的胶原蛋白网络现在被树脂在混合层中均匀而均匀地包裹在 用牙齿形成机械强大的锚。随后，纳米辅助表面上的吊坠功能 通过引入交联，将进一步提高树脂丁丁界面的强度和胶原蛋白的稳定性 通过初级和次要键。可能诱导分子间和不稳定的官能团的存在 纤维纤维间胶原蛋白交联以及疏水相互作用可以增强机械性能和 粘合剂层的生物构成。交联增加还将降低杂交层对细菌的敏感性 胶原酶和MMP。因此，我们将专门针对1）合成4至6个可以使用的光响应性纳米递归 被纳入常规粘合剂响应式和研究粘合剂树脂推进，作为430-480 nm光的函数 在模拟的牙髓压力下，暴露，聚合动力学，转化程度和机械性能。 为此，将使用溶液聚合协议和该方案合成光响应性的纳米递归。 将根据光响应部分的类型和浓度来表征粘合树脂纳米添加网络 在网络中。通过纳米递录引入的吊坠功能，以增强生物粘附和胶原蛋白交联 也将评估。在AIM 2中，我们将确定纳米辅助剂可以增强生物粘附的条件 在杂种层内通过附加的交联，并表征µTB，界面间隙的形成和稳定性 口腔生理化学环境中的树脂齿界面。