Low-cost Production of a Vaccine for Lyme Disease in Maize

低成本生产玉米莱姆病疫苗

• 批准号: 10362617
• 负责人: Erin Egelkrout
• 金额: $ 31.07万
• 依托单位: APPLIED BIOTECHNOLOGY INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-03-02 至 2024-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10362617
• 关键词: Address; Agrobacterium; Animal Feed; Animals; Antibodies; Antigens; Arthropod Vectors; Backcrossings; Bacteria; Benchmarking; Blood; Borrelia burgdorferi; Borrelia burgdorferi Group; Case Study; Cell Wall; Centers for Disease Control and Prevention (U.S.); Code; Communities; Data; Development; Disease; Disease Vectors; Encapsulated; Endoplasmic Reticulum; Enzyme-Linked Immunosorbent Assay; Escherichia coli; Feasibility Studies; Female; Flour; Gastrointestinal tract structure; Goals; Grain; Health; Human; Immune response; Immunization; Immunoglobulin G; Inbreeding; Infection; Ingestion; Injections; Ixodes; Lead; Lipids; Lyme Disease; Lyme Disease Vaccines; Maize; Membrane Proteins; Methods; Midwestern United States; Mus; Natural regeneration; Nymph; Oral; Oral Administration; Order Spirochaetales; OspA protein; Parents; Peptide Signal Sequences; Peromyscus; Phase; Plants; Prevalence; Prevention; Production; Recombinants; Reporting; Rodent; Route; Safety; Seeds; Small Business Innovation Research Grant; Subunit Vaccines; System; Testing; Ticks; Tissues; Tobacco; United States National Institutes of Health; Vaccinated; Vaccination; Vaccine Antigen; Vaccine Production; Vaccines; Western Blotting; White-Footed Mouse; Work; Zoonoses; base; commercialization; companion animal; cost; disease transmission; enzootic; feeding; follow-up; genetic selection; interest; male; new technology; novel; oral vaccine; plant genetics; scale up; synergism; tick feeding; tick-borne; transmission process; transmission-blocking vaccine; vaccine access; vaccine candidate; vaccine delivery; vaccine development; vaccine effectiveness; vaccine efficacy; vector; vector tick

PROJECT SUMMARY/ABSTRACT In keeping with the NIH’s stated priority for the development of vaccines to bacterial zoonoses, our goal is to develop an effective and conveniently delivered vaccine to protect against the spirochaete, Borrelia burgdorferi, the causative agent of Lyme disease. The bacterium is vectored by infected Ixodes species ticks between humans and mammalian reservoirs. Our chosen antigen candidate is OspA, a surface protein which presents as the spirochaete transits between the arthropod vector and the mammalian host. Vaccination with this antigen allows protective antibodies to encounter the spirochaete while still inside the tick as it engorges with a blood meal, and has been shown to be effective as a transmission blocking vaccine. In order to develop adequate amounts of antigen for an effective and adequate immune response, we have established a proprietorial expression system using recombinant maize. An additional advantage of maize expression is that vaccine antigens can be directly administered through the oral route rather than purified for injection. In this proposal, our goal is to produce large amounts of OspA antigen candidate in maize, orally administer the antigen to mice in feed formulated from recombinant maize flour, and demonstrate that the antigen elicits a protective immune response against challenge.

项目概要/摘要 根据 NIH 声明的开发细菌性人畜共患病疫苗的优先事项，我们的目标是 开发一种有效且方便的疫苗来预防螺旋体、伯氏疏螺旋体、 莱姆病的病原体是由受感染的硬蜱传播的。 人类和哺乳动物的宿主。 我们选择的候选抗原是 OspA，一种表面蛋白，当螺旋体在 用这种抗原进行疫苗接种可以使节肢动物载体和哺乳动物宿主产生保护性抗体。 当蜱虫吸食血粉时，当蜱虫仍在体内时，就会遇到螺旋体，并且已被证明 作为一种有效的传播阻断疫苗。 为了开发足够量的抗原以实现有效和充分的免疫反应，我们有 使用重组玉米建立了专有的表达系统。 表达的是疫苗抗原可以通过口服途径直接施用，而不是纯化用于 在此提案中，我们的目标是通过口服方式在玉米中生产大量 OspA 候选抗原。 将重组玉米粉饲料配方中的抗原给予小鼠，并证明该抗原 引发针对挑战的保护性免疫反应。

项目成果

Necroptosis Contributes to Persistent Inflammation During Acute Leptospirosis.

• DOI: 10.3389/fimmu.2022.810834
• 发表时间: 2022
• 期刊: Frontiers in immunology
• 影响因子: 7.3
• 作者: Kundu S;Shetty A;Gomes-Solecki M
• 通讯作者: Gomes-Solecki M

Borrelia burgdorferi (Spirochaetales: Spirochaetaceae) Infection Prevalence and Host Associations of Ticks Found on Peromyscus spp. in Maryland.

伯氏疏螺旋体（螺旋体目：螺旋体科）感染流行率和白鼠属蜱虫宿主关联。

• DOI: 10.1093/jme/tjab206
• 发表时间: 2022
• 期刊: Journal of medical entomology
• 影响因子: 2.1
• 作者: Poje,JuliaE;Azevedo,JoseF;Nair,Nisha;Mahachi,Kurayi;Frank,LexiE;Sherpa,Phurchhoki;Krizek,RachelS;Baccam,Tyler;Gomes-Solecki,Maria;Petersen,ChristineA
• 通讯作者: Petersen,ChristineA