Advancing Neuroimaging in Nonhuman Primates

推进非人类灵长类动物的神经影像学

基本信息

批准号：
9978306
负责人：
JAMES B DAUNAIS
金额：
$ 21.23万
依托单位：
WAKE FOREST UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-04-15 至 2022-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9978306
关键词：
Address Affect Alcohol abuse Alcohol consumption Anatomy Anesthesia procedures Animals Applications Grants Area Attention Behavioral Brain Brain region Cercopithecus tantalus Characteristics Chronic Clinical Cognition Cognitive Conscious Controlled Study Data Development Disease model Dose Environment Ethanol Female Foundations Frequencies Functional Magnetic Resonance Imaging Future Goals Grant Hippocampus (Brain)Human Image Magnetoencephalography Maps Measures Methods Modeling Monkeys Morphologic artifacts Motor Movement Performance Phenotype Prefrontal Cortex Procedures Reporting Research Rest Sampling Scanning Self Administration Signal Transduction Task Performances Time Training Work alcohol effect alcohol exposure animal imaging awake base behavior measurement brain behavior chronic alcohol ingestion drinking frontal eye fields habituation human disease human imaging human model imaging study information processing insight neuroimaging nonhuman primate programs tool

项目摘要

PROJECT SUMMARY The overall objective of this exploratory R21 is to establish proof of principle for developing neuroimaging in conscious monkeys using magnetoencephalography (MEG). We will focus on image acquisition in the MEG environment since the MEG operates silently. This developmental project is a logical extension of our ongoing work, which has proven the feasibility of acquiring MEG in anesthetized monkeys. We recently reported that chronic ethanol (EtOH) self-administration significantly altered signal power of multiple bandwidths across the brain in a vervet monkey model of EtOH abuse (Rowland et al., 2017a). More recent MEG data suggest that Rich Club characteristics of baseline, pre-exposure functional networks predict future drinking phenotypes and power spectral analyses found that resting state brain activity also predicts future drinking. Early exposure to EtOH during a 3-month EtOH induction procedure (Grant et al., 2008) appears to affect predominantly frontal regions and these effects expand to more caudal and subcortical areas with continued drinking. These studies however were conducted under anesthesia, which can complicate interpretation of the results, making it difficult to disentangle the effects of EtOH alone from potential EtOH-anesthesia interactions. We propose to compare brain activity and functional connectivity in fully conscious monkeys to characterize baseline, pre- ethanol brain function and functional networks and then to identify which brain regions are more vulnerable to the early effects of EtOH. Female vervet monkeys will be scanned in the fully conscious state using habituation procedures as outlined in this application. While the monkeys are habituated to conscious imaging, they will be trained to perform a delayed-match-to-sample (DMS) behavioral task as a behavioral measure of EtOH's effects on cognition and motor function. DMS performance measures will be collected throughout the study. Monkeys will be scanned while EtOH naïve, in the task-free state and then during active DMS performance. After baseline MEG acquisition in the pre-EtOH state, the monkeys will begin to drink EtOH using a well- characterized induction procedure during which monkeys drink escalating doses (0.5, 1.0 and 1.5 g/kg) EtOH for 30 days at each dose. MEG will be repeated after completion of each 30 day epoch to determine how dose- and time of exposure (30 days at each dose) affect brain function. The monkeys will be trained to perform a delayed match to sample behavioral task to track how EtOH affects cognitive and motor function as it relates to brain function. During each MEG recording session (EtOH naïve state and after each dose of EtOH), resting state brain function will be recorded followed by acquisition during task performance. The overarching goal of this developmental project is to extend conscious imaging combined with performance on behavioral tasks in our current research program to investigate how and when voluntary alcohol consumption affects functional brain networks in monkeys.

项目摘要该探索性R21的总体目的是建立开发神经影像学原理的证据有意识的猴子使用磁脑电图（MEG）。我们将重点介绍MEG中的图像获取由于MEG默默运作，因此环境。这个发展项目是我们正在进行的逻辑扩展工作证明了在麻醉猴子中获取MEG的可行性。我们最近报道了慢性乙醇（ETOH）自我管理显着改变了整个多个带宽的信号功率大脑在eTOH滥用的Vervet猴子模型中（Rowland等，2017a）。最近的MEG数据表明基线，暴露前功能网络的丰富俱乐部特征预测未来的饮酒表型和功率谱分析发现，静止状态大脑活动还可以预测未来的饮酒。早期接触 ETOH在3个月的EtOH诱导过程中（Grant等，2008）似乎主要影响正面随着持续饮酒，区域和这些影响扩展到更尾部和皮层下区域。这些研究但是，是在麻醉下进行的，这可能使结果复杂化，使得难以将单独的ETOH的影响与潜在的EtoH麻醉相互作用相互作用。我们建议比较完全有意识的猴子中的大脑活动和功能连通性，以表征基线乙醇脑功能和功能网络，然后确定哪些大脑区域更容易受到影响 ETOH的早期作用。雌性猴子将使用习惯在完全有意识的状态下扫描该应用程序中概述的程序。虽然猴子习惯于有意识的想象，但它们将是经过训练，可以执行延迟匹配样本（DMS）行为任务作为ETOH的行为度量对认知和运动功能的影响。在整个研究过程中，将收集DMS性能指标。在Etoh幼稚的情况下，在无任务状态下，然后在主动DMS性能期间，将扫描猴子。基线MEG在前eTOH状态中获取后，猴子将开始使用良好的特征诱导程序在此过程中，猴子饮料不断升级的剂量（0.5、1.0和1.5 g/kg）每剂量30天。每30天时期完成后将重复MEG，以确定剂量如何暴露时间（每次剂量30天）会影响大脑功能。猴子将接受训练以表演延迟匹配到样本行为任务，以跟踪ETOH如何影响认知和运动功能大脑功能。在每次MEG录制会议（EtoH幼稚的状态和每剂ETOH之后）期间，休息状态大脑功能将记录在任务执行期间的收购。总体目标这个发展项目是扩展有意识的成像与行为任务的性能相结合我们当前的研究计划，以研究自愿饮酒的方式以及何时影响功能猴子中的大脑网络。