Shared Resource - Animal Models Shared Resource

共享资源 - 动物模型共享资源

基本信息

批准号：
9924507
负责人：
CHRISTOPHER ALBANESE
金额：
$ 13.85万
依托单位：
GEORGETOWN UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/9924507
关键词：
Academic Medical Centers Accreditation Aging Agriculture Alleles Alzheimer&apos s Disease Alzheimer&apos s disease risk American Anatomy Animal Cancer Model Animal Experiments Animal Model Animal Technicians Animals Antineoplastic Agents Apolipoprotein E Autopsy Bioinformatics Shared Resource Biometry Brain Breast Cancer Cell Breast Epithelial Cells Cancer Center Support Grant Cancer Control Carcinogens Cells Comprehensive Cancer Center Data Diagnostic radiologic examination Diet Doctor of Philosophy Dominant-Negative Mutation Drug Screening Drug toxicity Endocrine Estrogen receptor positive Ewings sarcoma Funding Generations Genes Genotype Goals Grant Histopathology Housing Human Human Resources Image Image Analysis Immune system Immunocompetent Immunocompromised Host In Vitro International Investigation Investigational Therapies Knowledge Laboratory Animal Science Laboratory Animals Letters Link Magnetic Resonance Imaging Maintenance Malignant Neoplasms Malignant neoplasm of pancreas Mammary Neoplasms Mammary gland Medicine Modeling Monitor Mus National Cancer Institute Neoplasm Metastasis Neuroblastoma Nipples Normal Cell Oral Performance Pharmaceutical Preparations Predisposition Primary Neoplasm Proteins Publications Rattus Research Research Personnel Resistance Resolution Resource Sharing Resources Rodent Rodent Model Role Services Signal Transduction Source Tail Techniques Testing Tissues Training Translational Research Ultrasonography United States National Institutes of Health Universities Veins Work Xenograft procedure Zebrafish animal care animal facility animal imaging anticancer research base cancer cell cancer initiation cancer prevention cognitive performance comparative design experimental study high risk hormone therapy in vivo in vivo Model knockout gene malignant breast neoplasm member metabolic profile molecular oncology morphometry mouse model neuropeptide Y novel novel therapeutics overexpression pancreatic cancer model preclinical study prevent programs response skills spectroscopic imaging success tool translational study tumor tumor growth tumor initiation

项目摘要

ABSTRACT Animal models are powerful cancer research tools that are vital to translational research. The function of the Animal Models Shared Resource (AMSR) is to facilitate the efficient, economical, state-of-the-art use of animals for the performance of cancer-related studies. This is accomplished through a centralized resource in which American Association for Laboratory Animal Science–certified, highly trained veterinary technicians provide translational research services on animals to members of the Georgetown Lombardi Comprehensive Cancer Center (LCCC). A significant emphasis is placed on assisting users with the design and performance of animal studies using a wide range of models extending from zebrafish to an estrogen receptor-positive rat for studying breast cancer. Major services include all aspects of rodent studies, such as establishing genetically modified mouse colonies; administering carcinogens; monitoring tumor growth; collecting tissues and tumors at necropsy and administering drugs, diets or other compounds. Studies using zebrafish include generating gene knockout or gene overexpressing models, screening drugs for toxicity and conducting xenotransplantation experiments (i.e., “zevatars”—zebrafish avatar). Imaging services are also provided to monitor tumor initiation, progression and response to therapy. The imaging services provided by the AMSR include high-resolution, in vivo, small animal imaging and image analysis using a Bruker 7 tesla magnetic resonance imaging and spectroscopy console, a small animal ultrasound (Vevo 660) workstation, an x-ray imaging unit (Faxitron) and a bioluminescent and visible to near-infrared spectroscopic imaging workstation (IVIS Lumina III). The AMSR is located within Georgetown University Medical Center's (GUMC’s) Division of Comparative Medicine (DCM). The DCM is a centralized, U.S. Department of Agriculture-registered animal facility that is accredited by the Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC) International and that has an approved letter of assurance on file at the National Institutes of Health (NIH). In fiscal year 2017, the AMSR provided services to 24 LCCC members from all four Research Programs (Breast Cancer [BC], Cancer Prevention and Control [CPC], Experimental Therapeutics [ET] and Molecular Oncology [MO]). The AMSR works closely with the Histopathology & Tissue Shared Resource (HTSR) and the Biostatistics & Bioinformatics Shared Resource (BBSR). The AMSR supported 50 publications in the funding period. The AMSR is codirected by Christopher Albanese, PhD, Leena Hilakivi-Clarke, PhD, and Eric Glasgow, PhD, who oversee imaging, rodent and zebrafish components, respectively.

抽象的动物模型是强大的癌症研究工具，对翻译研究至关重要。函数动物模型共享资源（AMSR）是为了促进动物的有效，经济，最先进的使用为了进行与癌症相关的研究。这是通过集中资源完成的美国实验室动物科学协会 - 认可，训练有素的兽医技术人员提供对动物的转化研究服务给乔治敦伦巴第综合癌症的成员中心（LCCC）。重点是协助用户进行动物的设计和性能使用从斑马鱼到雌激素受体阳性大鼠扩展的广泛模型的研究乳腺癌。主要服务包括啮齿动物研究的所有方面，例如建立遗传修饰小鼠菌落；管理致癌物；监测肿瘤生长；在尸检时收集组织和肿瘤并施用药物，饮食或其他化合物。使用斑马鱼的研究包括产生基因敲除或基因过表达模型，筛查毒性和进行异种移植实验的药物（即“ Zevatars” - Zebrafish Avatar）。还提供了成像服务以监测肿瘤的启动，进展和对治疗的反应。 AMSR提供的成像服务包括高分辨率，体内，小动物成像和图像分析使用Bruker 7 Tesla磁共振成像和光谱控制台，一个小动物超声（VEVO 660）工作站，X射线成像单元（Faxitron）和生物发光并可见近红外光谱成像工作站（Ivis Lumina III）。 AMSR位于乔治敦大学医学中心（GUMC）比较医学部（DCM）。 DCM是美国农业登记部的集中式动物设施，该设施得到了协会的认可国际实验室动物护理（AAAALAC）的评估和认证，并获得了批准美国国立卫生研究院（NIH）的保证书。在2017财政年度，AMSR提供为来自所有四项研究计划（乳腺癌[BC]，癌症预防和对照[CPC]，实验疗法[ET]和分子肿瘤学[MO]）。 AMSR与组织病理学和组织共享资源（HTSR）以及生物统计学和生物信息学共享资源（BBSR）。 AMSR在资助期间支持50个出版物。 AMSR由克里斯托弗（Christopher）指导 Albanese，PhD，Leena Hilakivi-Clarke博士和埃里克·格拉斯哥（Eric Glasgow）斑马鱼的组成部分。