Research Support Core: Genetics and Metabolomics

研究支持核心：遗传学和代谢组学

基本信息

批准号：
9903334
负责人：
PAMELA L MELLON
金额：
$ 26.49万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN DIEGO
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/9903334
关键词：
Address Amendment Animal Model Bioinformatics Biological Biological Assay Biomedical Research Biosensor CRISPR/Cas technology ChIP-seq Collaborations Communities Core Facility DNA Data Detection Disease Ecology Edible Plants Embryo Environment Environmental Engineering technology Environmental Health Equipment Fatty Liver Feces Gene Expression Generations Genetic Goals Growth Factor Health Hormones Human Human Resources Informatics Injections Intervention Laboratories Liver diseases Measurement Measures Methods Microinjections Mission Modeling Modification Molecular Molecular Biology Mus Mutagenesis Physiological Plants Plasma Positioning Attribute Preparation Prevention strategy Problem Solving Research Research Personnel Research Project Grants Research Support Risk Role Sampling Serum Services Sociology Soil Statistical Data Interpretation Steatohepatitis Superfund System Technology Tissues Toxic Environmental Substances Toxicant exposure Training Translational Research Urine Water Work analytical tool base blastocyst chromatin modification computer program cost cost effective cytokine design driving force embryonic stem cell genetic manipulation genome-wide health disparity high throughput analysis high throughput screening homologous recombination in vivo large datasets liquid chromatography mass spectrometry magnetic beads metabolomics mouse genome mouse model novel small molecule soil sampling success tool toxicant transcriptome sequencing transcriptomics water sampling

项目摘要

Core Summary/Abstract Studies of Superfund toxicants require novel mouse models, careful, high-throughput analyses of hormones, cytokines and small molecule metabolites, and informatics analyses of large datasets to pioneer new findings in the detection of environmental toxicants and health effects of exposure. The UC San Diego Superfund Research Center Genetics and Metabolomics Core facility will provide state-of-the-art molecular biology services for generation of murine models of toxicant exposure as well as cutting-edge analytical services for sensitive, high-throughput assay of hormones and small molecule metabolites and their informatic analyses. This Core will interface with all six proposed research projects. Comprehensive approaches for modifying the mouse genome are provided including DNA microinjection, embryonic stem cell homologous recombination, CRISPR/Cas9 mutagenesis, and blastocyst injection, specifically tailored to UCSD Superfund projects. Furthermore, metabolomics services will be provided, allowing for measure of hormones, growth factors, and thousands of small molecule metabolites (both targeted and untargeted) using a Luminex magnetic bead analyzer and liquid chromatography-mass spectrometry based approaches. Samples for analysis will include human and mouse biospecimens (plasma, urine, and stool) as well as community plant, water, and soil samples. Bioinformatics services will provide in-depth analyses of large datasets resulting from transcriptomics, cistromics, and metabolomics. This core provides centralized, cost-effective, efficient, technically sophisticated services that are crucial to the success of all six projects in the UC San Diego Superfund Research Center.

核心摘要/摘要超级基金有毒物质的研究需要新颖的小鼠模型，仔细的，高通量的激素分析，细胞因子和小分子代谢物，以及大型数据集的信息分析，以开创新发现在检测环境有毒物质和暴露的健康影响中。加州大学圣地亚哥超级基金研究中心遗传学和代谢组学核心设施将提供最先进的分子生物学用于生成毒剂暴露的鼠模型以及最先进的分析服务的服务对激素和小分子代谢产物的敏感，高通量测定及其信息分析。该核心将与所有六个建议的研究项目接口。修改的全面方法提供了小鼠基因组，包括DNA显微注射，胚胎干细胞同源重组， CRISPR/CAS9诱变和胚泡注射，专门针对UCSD超级基金项目量身定制。此外，还将提供代谢组学服务，以衡量激素，生长因素和使用Luminex磁珠分析仪和液相色谱 - 质谱法。用于分析的样本将包括人和小鼠生物测量（血浆，尿液和粪便）以及社区植物，水和土壤样品。生物信息学服务将对由大型数据集提供深入分析转录组学，Cistromics和代谢组学。该核心提供集中，成本效益，高效，技术复杂的服务对于圣地亚哥分校的所有六个项目至关重要超级基金研究中心。