Biospecimen and Pathology Core

生物样本和病理学核心

基本信息

批准号：
9759782
负责人：
Andrea Marcogliese Sheehan
金额：
$ 34.25万
依托单位：
BAYLOR COLLEGE OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/9759782
关键词：
Antigen Targeting B-Lymphocytes Bioinformatics Biological Assay Biology Biopsy Blood Cells Blood specimen Breast Lymphoma CLIA certified Cells Chromosomes Classification Clinical Clinical Research Clinical Trials Comprehensive Cancer Center Computer software Cytogenetics Databases Detection Detection of Minimal Residual Disease Development Diagnostic tests Drug or chemical Tissue Distribution Enrollment Ensure Flow Cytometry Freezing Fresh Tissue Funding Future Gene Chips General Hospitals Genes Genetic Goals Hematopathology Histology Hodgkin Disease Hospitals Immunohistochemistry In Situ Hybridization In Vitro Institution Laboratories Lead Lymphoma Medical center Medicine Methodist Church Microarray Analysis Modeling Molecular Non-Hodgkin&apos s Lymphoma Online Systems Paraffin Paraffin Embedding Pathologist Pathology Patients Pediatric Hospitals Peripheral Plasma Population Research Personnel Research Project Grants Resource Allocation Resources Sampling Secure Services Site Specimen Stains System T-Lymphocyte Testing Texas Time Tissue Banks Tissue Microarray Tissue Sample Tissues Translational Research Translations Validation Xenograft procedure base biobank bioinformatics resource cancer genetics career college design exome sequencing experience experimental study follow-up genetic analysis genome sequencing in vivo in vivo Model laser capture microdissection lymph nodes neoplastic cell novel therapeutics preclinical study repository research and development research clinical testing research study tissue preparation transcriptome sequencing tumor viral RNA virtual

项目摘要

PROJECT SUMMARY/ ABSTRACT The purpose of this resource is to provide tissue, cells and pathology services to all 4 research projects and career enhancement or research development awardees covered by this application. To achieve this goals the Lymphoma SPORE is combines pathology resources of the Dan L Duncan Comprehensive Cancer Center (DLDCC) at Baylor College of Medicine (BCM) and its affiliated institutions with those of Houston Methodist Hospital to ensure adequate numbers and timely delivery of tissue/blood samples for the projected needs of project investigators. It therefore co-ordinates acquisition of tissue for banking from lymphoma and CLL patients at affiliated hospitals in the Texas Medical Center, by relying on established tissue collection and distribution systems. The capacity to obtain samples from multiple affiliated hospitals will greatly increase the number of specimens we are able to analyze, store and distribute. The core also provides pathology services including routine tissue preparation, immunohistochemical staining, laser capture microdissection, preparation of tissue microarrays, flow cytometry, detection of minimal residual disease and in situ hybridization for viral RNAs. These tasks are essential to the successful completion of planned experiments in each project and will be performed by expert pathologists and histotechnologists with substantial experience in providing core services to SPORE investigators. It also co-ordinates supply of tissue to the Patient-Derived Xenograft and Advanced In Vivo Models Core of the DLDCC. This core also accesses a cancer genetics laboratory for specialized molecular studies including FISH and sequencing and bioinformatics support to support tissue-banking software needs for the lymphoma SPORE. Bioinformatics support comes from the DLDCC Bioinformatics Resource that utilizes Acquire, a secure web- based modular application that serves as the unifying hub for all DLDCC-based biorepositories, including the BCM Breast and Lymphoma SPOREs. In the last funding period the Resource Allocation Committee approved 30 requests from SPORE and external investigators for tissue or blood samples.

项目概要/摘要该资源的目的是为所有 4 个研究项目提供组织、细胞和病理学服务本申请涵盖的职业提升或研究发展获奖者。为了实现这个目标淋巴瘤 SPORE 结合了 Dan L Duncan 综合癌症中心的病理学资源贝勒医学院 (BCM) 中心 (DLDCC) 及其附属机构与休斯顿的附属机构卫理公会医院确保为患者提供足够数量和及时的组织/血液样本项目调查员的预计需求。因此，它协调从银行获取组织德克萨斯医学中心附属医院的淋巴瘤和 CLL 患者，依靠现有的组织收集和分配系统。具备多家附属医院样本获取能力将大大增加我们能够分析、储存和分发的样本数量。核心还提供病理学服务，包括常规组织制备、免疫组织化学染色、激光捕获显微切割、组织微阵列制备、流式细胞术、最小残留检测疾病和病毒RNA原位杂交。这些任务对于顺利完成至关重要每个项目中计划的实验将由病理学家和组织技术专家进行在为 SPORE 调查员提供核心服务方面拥有丰富的经验。它还协调供应组织到 DLDCC 的患者来源异种移植和高级体内模型核心。这个核心还进入癌症遗传学实验室进行专门的分子研究，包括 FISH 和测序和生物信息学支持，以满足淋巴瘤 SPORE 的组织库软件需求。生物信息学支持来自 DLDCC 生物信息学资源，该资源利用 Acquire（一个安全的网络）基于模块化应用程序，作为所有基于 DLDCC 的生物样本库的统一中心，包括 BCM 乳房和淋巴瘤孢子。在上一个资助期间，资源分配委员会批准了 SPORE 和外部研究人员提出的 30 项组织或血液样本请求。