Core D: Biospecimen and Pathology Core

核心 D：生物样本和病理学核心

基本信息

批准号：
10495082
负责人：
Andrea Marcogliese Sheehan
金额：
$ 28.87万
依托单位：
BAYLOR COLLEGE OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-09-11 至 2027-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10495082
关键词：
Antigen Targeting B-Lymphocytes Bioinformatics Biological Assay Biopsy Blood Cells Blood specimen Breast Lymphoma CLIA certified Catchment Area Cells Chromosomes Chronic Lymphocytic Leukemia Classification Clinical Clinical Research Clinical Trials Comprehensive Cancer Center Computer software Cytogenetics Databases Detection of Minimal Residual Disease Development Diagnostic tests Drug or chemical Tissue Distribution Enrollment Ensure Flow Cytometry Freezing Fresh Tissue Funding Future Gene Chips General Hospitals Genes Genetic Goals Hematopathology Histology Hodgkin Disease Hospitals Immunohistochemistry In Situ Hybridization In Vitro Institution Laboratories Lymphoma Lymphoma cell Medical center Medicine Methodist Church Microarray Analysis Molecular Non-Hodgkin&apos s Lymphoma Online Systems Paraffin Paraffin Embedding Pathologist Pathology Patients Pediatric Hospitals Peripheral Plasma Population Research Personnel Research Project Grants Research Subjects Resource Allocation Resources Sampling Secure Services Site Specimen Stains Support Groups System T-Lymphocyte Testing Texas Time Tissue Banks Tissue Microarray Tissue Sample Tissues Translational Research Translations Validation base biobank biomedical informatics cancer genetics career college design exome sequencing experience experimental study follow-up genetic analysis genome sequencing in vivo in vivo Model laser capture microdissection leukemia/lymphoma lymph nodes neoplastic cell novel therapeutic intervention patient derived xenograft model preclinical study repository research and development research clinical testing research study tissue preparation transcriptome sequencing tumor viral RNA virtual

项目摘要

PROJECT SUMMARY/ ABSTRACT The purpose of this Biospecimen and Pathology Core is to provide tissue, cells and pathology services to the three research projects and career enhancement, or research development awardees covered by this application. To achieve these goals, the Lymphoma SPORE combines pathology resources from the Dan L Duncan Comprehensive Cancer Center (DLDCCC) at Baylor College of Medicine (BCM) and its affiliated institutions with those of Houston Methodist Hospital to ensure adequate numbers and timely delivery of tissue/blood samples for the projected needs of SPORE and external investigators. The proposed core resource will therefore co-ordinate acquisition of tissue for banking from lymphoma and CLL patients at affiliated hospitals in the Texas Medical Center, by relying on established tissue collection and distribution systems. The capacity to obtain samples from multiple affiliated hospitals will greatly increase the number of specimens we are able to analyze, store and distribute from our diverse catchment area. The core also provides pathology services including routine tissue preparation, immunohistochemical staining, laser capture microdissection, preparation of tissue microarrays, flow cytometry, detection of minimal residual disease and in situ hybridization for viral RNAs. These tasks are essential to the successful completion of planned experiments in each project and will be performed by expert pathologists and histotechnologists with substantial experience in providing core services to SPORE investigators. This core is also responsible for coordinating supply of tissue to the Patient-Derived Xenograft and Advanced In Vivo Models Core of the DLDCCC, resources that will be of particular use to our investigators. This core also accesses a cancer genetics laboratory for specialized molecular studies including FISH and sequencing and bioinformatics support to support tissue-banking software needs for the lymphoma SPORE. Bioinformatics support comes from the DLDCCC Biomedical Informatics Group that supports OpenSpecimen, a secure web-based modular application that serves as the unifying hub for all DLDCCC-based biorepositories, including the BCM Breast and Lymphoma SPOREs. In the last funding period, the Resource Allocation Committee approved 25 requests from SPORE and external investigators for tissue or blood samples.

项目概要/摘要该生物样本和病理学核心的目的是为生物样本和病理学提供组织、细胞和病理学服务。三个研究项目和职业提升，或本研究涵盖的研究开发获奖者应用。为了实现这些目标，淋巴瘤 SPORE 结合了 Dan L 的病理学资源贝勒医学院 (BCM) 邓肯综合癌症中心 (DLDCCC) 及其附属机构机构与休斯敦卫理公会医院的机构合作，确保足够的数量和及时交付组织/血液样本，以满足 SPORE 和外部研究人员的预计需求。拟议的核心因此，资源将协调从淋巴瘤和 CLL 患者那里获取组织用于储存德克萨斯医学中心的附属医院，依靠现有的组织收集和分发系统。多家附属医院获取样本的能力将大大增加我们能够分析、储存和分发来自不同流域的样本。核心还提供病理学服务，包括常规组织制备、免疫组织化学染色、激光捕获显微切割、组织微阵列制备、流式细胞术、微小残留病检测和病毒RNA的原位杂交。这些任务对于顺利完成计划任务至关重要每个项目中的实验将由病理学家和组织技术专家进行在为 SPORE 调查人员提供核心服务方面拥有丰富的经验。该核心还负责协调患者来源的异种移植物和高级体内模型核心的组织供应 DLDCCC，对我们的调查人员特别有用的资源。这个核心也接触到了癌症用于专业分子研究的遗传学实验室，包括 FISH、测序和生物信息学支持淋巴瘤 SPORE 的组织库软件需求。生物信息学支持来了来自支持 OpenSpecimen 的 DLDCCC 生物医学信息学小组，OpenSpecimen 是一种基于网络的安全模块化模块作为所有基于 DLDCCC 的生物样本库（包括 BCM Breast）的统一中心的应用程序和淋巴瘤孢子。在上一个资助期内，资源分配委员会批准了 25 SPORE 和外部研究人员索取组织或血液样本的请求。