Optical Imaging Tools for Elucidating the Roles of Anions and Anionic Modifications in Cellular Signaling

用于阐明阴离子和阴离子修饰在细胞信号传导中的作用的光学成像工具

• 批准号: 9752644
• 负责人: Sheel Dodani
• 金额: $ 38.25万
• 依托单位: UNIVERSITY OF TEXAS DALLAS
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9752644
• 关键词: Anions; Binding; Biological; Biology; Cations; Cells; Charge; Chemicals; Chlorides; Cystic Fibrosis; Detoxification Process; Diagnosis; Disease; Engineering; Event; Goals; Health; Human; Imaging Device; Ions; Lead; Light; Malignant Neoplasms; Modification; Molecular; Organism; Pain; Proteins; Research; Role; Signal Transduction; Source; Technology; Unspecified or Sulfate Ion Sulfates; autism spectrum disorder; chronic pain; optical imaging; programs

PROJECT SUMMARY Cation fluxes are known to drive cellular signaling events, whereas anions are considered to function as counterions to neutralize these changes. But could anions be dynamic cellular signals? This paradigm shifting question, in large part, has been overlooked from the chemical biology/bioinorganic perspective, despite the fact that anion dysregulation is implicated in a variety of diseases including chronic pain, autism, and cancer. The long-term goal of our research program is to identify the cellular sources, protein targets, and signaling roles of biologically relevant anions in health and disease, but the technologies to do so are still underdeveloped. To this end, here we propose to create new optical imaging tools that will allows to build a molecular level picture into the how, when, where, and why of what anions are actually doing in living systems. Our findings, will not only provide a fundamental understanding for the role of anions in cell signaling, but also in the manifestation, diagnosis, and treatment of associated diseased states.

项目摘要 已知阳离子通量会驱动细胞信号事件，而阴离子被认为起作用 抵消这些变化的反击。但是阴离子可以是动态的细胞信号吗？这个范式转移 从化学生物学/生物无机的角度来看 阴离子失调与多种疾病有关，包括慢性疼痛，自闭症和癌症。这 我们的研究计划的长期目标是确定细胞来源，蛋白质靶标和信号传递作用 在健康和疾病中与生物学相关的阴离子，但是这样做的技术仍然不发达。对此 最后，我们建议创建新的光学成像工具，以允许在 如何，何时，何时何地以及为什么在生活系统中实际在做什么。我们的发现，不仅会 对阴离子在细胞信号传导中的作用以及在表现中的作用提供基本的理解， 诊断和治疗相关状态。