Optical Imaging Tools for Elucidating the Roles of Anions and Anionic Modifications in Cellular Signaling

用于阐明阴离子和阴离子修饰在细胞信号传导中的作用的光学成像工具

• 批准号: 9895385
• 负责人: Sheel Dodani
• 金额: $ 23.57万
• 依托单位: UNIVERSITY OF TEXAS DALLAS
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9895385
• 关键词: Anions; Binding; Biological; Biology; Cations; Cells; Charge; Chemicals; Chlorides; Cystic Fibrosis; Detoxification Process; Diagnosis; Disease; Engineering; Event; Goals; Health; Human; Imaging Device; Ions; Lead; Light; Malignant Neoplasms; Modification; Molecular; Organism; Pain; Proteins; Research; Role; Signal Transduction; Source; Technology; Unspecified or Sulfate Ion Sulfates; autism spectrum disorder; chronic pain; optical imaging; programs

PROJECT SUMMARY Cation fluxes are known to drive cellular signaling events, whereas anions are considered to function as counterions to neutralize these changes. But could anions be dynamic cellular signals? This paradigm shifting question, in large part, has been overlooked from the chemical biology/bioinorganic perspective, despite the fact that anion dysregulation is implicated in a variety of diseases including chronic pain, autism, and cancer. The long-term goal of our research program is to identify the cellular sources, protein targets, and signaling roles of biologically relevant anions in health and disease, but the technologies to do so are still underdeveloped. To this end, here we propose to create new optical imaging tools that will allows to build a molecular level picture into the how, when, where, and why of what anions are actually doing in living systems. Our findings, will not only provide a fundamental understanding for the role of anions in cell signaling, but also in the manifestation, diagnosis, and treatment of associated diseased states.

项目概要 已知阳离子通量可驱动细胞信号传导事件，而阴离子则被认为具有以下作用： 对抗来抵消这些变化。但阴离子可以是动态细胞信号吗？这种范式转变 尽管事实上，从化学生物学/生物无机的角度来看，这个问题在很大程度上被忽视了 阴离子失调与多种疾病有关，包括慢性疼痛、自闭症和癌症。这 我们研究计划的长期目标是确定细胞来源、蛋白质靶点和信号作用 阴离子在健康和疾病中具有生物学相关性，但实现这一点的技术仍然不发达。对此 最后，我们建议创建新的光学成像工具，将分子水平的图像构建到 阴离子在生命系统中实际发挥作用的方式、时间、地点和原因。我们的研究结果不仅会 提供对阴离子在细胞信号传导中以及表现形式中的作用的基本理解， 相关疾病状态的诊断和治疗。