High Resolution High Throughput Proteomics Platform for Cancer Research

用于癌症研究的高分辨率高通量蛋白质组学平台

基本信息

批准号：
9751068
负责人：
Yehia Ibrahim
金额：
$ 42.57万
依托单位：
BATTELLE PACIFIC NORTHWEST LABORATORIES
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-08-01 至 2021-07-31
项目状态：
已结题

项目摘要

PROJECT SUMMARY/ABSTRACT The objective of this project is to evaluate a new high-throughput and ultra-sensitive platform that will enable more effective and higher throughput proteomics measurements to support and advance cancer research. The new platform will provide substantially higher resolution ion mobility spectrometry (IMS) separations than current platforms. This work also leverages previous developments at our laboratory by combining these new capabilities with more sensitive and efficient ion source and high accuracy MS technologies. The resolution of the IMS achieved will be greatly increased by using electric traveling waves applied to an extremely long path design based upon Structures for Lossless Ion Manipulations (SLIM), while also enabling very high sensitivity to be achieved. The platform will provide approximately two orders of magnitude improvement in measurement throughput for similar or better measurement coverage, quantification, etc. over conventional condensed-phase liquid chromatography-mass spectrometry (LC-MS) platforms, enabling thousands of samples to be analyzed in hours, rather than weeks as at present. We will also adapt and refine bioinformatics tools developed in our laboratory to provide automated and efficient data processing. Finally, the platform will be evaluated utilizing complex clinical samples in terms of proteomic peptide/protein coverage, measurement throughput, CVs of replicate analyses and platform robustness and in comparison to leading-edge conventional LC-MS platforms available in our laboratory.

项目摘要/摘要该项目的目的是评估一个新的高通量和超敏感平台这将使更有效和更高的吞吐量蛋白质组学测量以支持和预先癌症研究。新平台将提供更高的分辨率离子移动光谱法（IMS）的分离比当前平台。这项工作也利用通过将这些新功能与更多的更多功能相结合，在我们实验室的先前发展敏感有效的离子源和高精度MS技术。 IMS的解决方案通过使用应用于极长的电波，实现的实现将大大增加基于无损离子操纵结构的路径设计（Slim），同时也可以要实现的敏感性非常高。该平台将提供大约两个订单相似或更好测量的测量吞吐量的幅度改善覆盖范围，定量等在常规凝聚相液相色谱质量上光谱法（LC-MS）平台，可以在数小时内分析成千上万的样本比目前的几周。我们还将适应和完善我们开发的生物信息学工具实验室提供自动化和有效的数据处理。最后，平台将是根据蛋白质组学肽/蛋白质覆盖范围，评估了使用复杂的临床样品，测量吞吐量，重复分析的简历和平台鲁棒性以及相比到我们实验室可用的领先传统LC-MS平台。